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anti-Rat (Rattus) Huntingtin antibody for Immunoprecipitation

Recommended Huntingtin Antibody (supplied by: Log in to see )

Antigen
Huntingtin (HTT) Antibodies
  • AI256365
  • C430023I11Rik
  • CG9995
  • dHtt
  • dhtt
  • Dmel\\CG9995
  • HD
  • hd
  • Hd
  • Hdh
  • Hsap\\HD
  • htt
  • HTT
  • Htt
  • huntington
  • it15
  • IT15
  • SLC6A4
  • ZHD
Epitope
AA 1844-2131
14
13
12
9
7
7
6
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
153
73
42
7
2
Host
Mouse
82
74
3
1
Clonality
Monoclonal
Conjugate
This Huntingtin antibody is un-conjugated
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
125
74
49
24
21
19
18
18
11
8
3
3
2
Supplier
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Catalog No. ABIN269391
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089465064 ABIN153591 IHC (fro) IP IHC WB Mouse IgG1 AA 997-1276 Log in to see HDA3E10
0.00089465064 ABIN269392 IHC (fro) IP IHC WB Mouse IgG AA 2703-2911 Log in to see HDC8A4
0.00089465064 ABIN2853713 IC IP IHC ELISA WB Mouse IgG1, kappa AA 2146-2541 Log in to see 1HU-4C8

Similar anti-Huntingtin Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 21 Antibodies 26 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies 2 Antibodies
Immunochromatography (IC) 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 9 Antibodies
Immunofluorescence (IF) 5 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 20 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 29 Antibodies

General

Antigen Huntingtin (HTT) Antibodies
Epitope AA 1844-2131
(14), (13), (12), (9), (7), (7), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(153), (73), (42), (7), (2)
Host Mouse
(82), (74), (3), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Huntingtin antibody is un-conjugated
(2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(125), (74), (49), (24), (21), (19), (18), (18), (11), (8), (3), (3), (2)
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Product Details anti-Huntingtin Antibody

Target Details Huntingtin Application Details Handling Images
Specificity This reacts with an epitope corresponding to the HDB region (amino acids 1844 - 2131) of the huntingtin protein.
Purification IgG purified
Immunogen Recombinant fragment, corresponding to amino acids 1844-2131 of Huntingtin.
Clone HDB4E10
Isotype IgG1

Target Details Huntingtin

Product Details anti-Huntingtin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Huntingtin (HTT Antibody Abstract)
Background The combined use of clones HDC8A4, HDB4E10 and HDA3E10 demonstrate thathuntingtin is enriched in neuronal cells in the brain. Alternate Names: anti-HD antibody, anti-HD protein antibody, anti-HDH antibody, anti-HTT antibody,anti-HUNTINGTON CHOREA antibody, anti-Huntington's disease protein homologantibody, anti-IT15 antibody, anti-ZHD antibody.
Gene Symbol: HTT
Gene ID 3064, 15194, 29424
Research Area Neurology, Virology
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-Huntingtin Antibody Target Details Huntingtin Handling Images back to top
Application Notes Immunohistology on frozen (paraffin not tested). Western blotting detects a 350kD band on western blots but also detects smaller degradation products of huntingtin. This clone recognizes both denatured and native huntingtin in human brain.
Recommended dilutions: Immunoprecipitation 1:10-1:500, Western Blot 1:100-1:2000, Immunohistochemistry-Frozen 1:10-1:2000, Immunohistochemistry 1:10-1:2000
Restrictions For Research Use only

Handling

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Images back to top
Concentration 1.0 mg/mL
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C
Storage Comment 4 °C short term. Aliquot and store at -20 °C long term.

Images

Product Details anti-Huntingtin Antibody Target Details Huntingtin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Huntingtin antibody (HTT) (AA 1844-2131) (ABIN269391) anti-Huntingtin (HTT) (AA 1844-2131) antibody