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anti-Rat (Rattus) MAP3K8 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended MAP3K8 Antibody (supplied by: Log in to see )

Antigen
Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies
  • c-COT
  • Cot
  • COT
  • Cot/Tpl2
  • D17TPL2
  • Est
  • EST
  • ESTF
  • Estf
  • MAP3K8
  • MEKK8
  • Tpl-2
  • TPL2
  • Tpl2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
93
37
34
5
4
2
1
1
Host
Rabbit
89
4
Clonality
Polyclonal
Conjugate
This MAP3K8 antibody is un-conjugated
5
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
49
41
32
22
13
11
7
5
3
2
Supplier
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Catalog No. ABIN265369
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089475926 ABIN746573 IF (p) IHC (p) WB Rabbit IgG AA 370-420, pSer400 Log in to see Polyclonal
0.00089475926 ABIN746588 IF (p) IHC (p) Rabbit IgG AA 270-320, pThr290 Log in to see Polyclonal
0.00089475926 ABIN701275 IF (p) IHC (p) WB Rabbit IgG AA 140-190 Log in to see Polyclonal
0.00089475926 ABIN701277 IHC (p) WB Biotin Rabbit IgG AA 140-190 Log in to see Polyclonal
0.00089475926 ABIN701284 IHC (p) WB HRP Rabbit IgG AA 140-190 Log in to see Polyclonal

Similar anti-MAP3K8 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 14 Antibodies 13 Antibodies 41 Antibodies
Immunochromatography (IC) 2 Antibodies 2 Antibodies 2 Antibodies
Immunofluorescence (IF) 4 Antibodies 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 14 Antibodies 14 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 6 Antibodies 8 Antibodies
Western Blotting (WB) 17 Antibodies 20 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 5 Antibodies
Dot Blot (DB) 11 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies

General

Antigen Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(93), (37), (34), (5), (4), (2), (1), (1)
Host Rabbit
(89), (4)
Clonality
Polyclonal
Conjugate This MAP3K8 antibody is un-conjugated
(5), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(49), (41), (32), (22), (13), (11), (7), (5), (3), (2)
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Product Details anti-MAP3K8 Antibody

Target Details MAP3K8 Application Details Handling Images
Specificity This antibody detects endogenous levels of MAP3K8 / TPL-2 protein(region surrounding Pro284).
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity Chromatography using epitope-specific immunogen

Target Details MAP3K8

Product Details anti-MAP3K8 Antibody Application Details Handling Images back to top
Antigen
Alternative Name MAP3K8 / TPL-2 (MAP3K8 Antibody Abstract)
Background The role of mitogen-activated protein kinases (MAPKs) in cell signaling pathways is well established. The rat gene Tpl-2, for tumor progression locus 2, and the human and mouse homologues c-Cot, for cancer osaka thyroid oncogene, encode a proto-oncogene serine/threonine protein kinase that was shown to play a role in the functional activation of the MAP kinase pathway. Overexpression of Cot induces MAP kinase activation in COS-1 and NIH/3T3 cells. Cot-mediated activation of MAP kinase is inhibited by both Ras N17, a dominant negative mutant of c-H-Ras, and Raf-1s621A, a dominant negative mutant of Raf-1, suggesting that Cot functions upstream of Ras and Raf-1.Other studies have shown that a kinase-negative, dominant negative mutant of Cot partially blocks Ras or Raf-1-induced MAP kinase activation, arguing that Cot functions downstream of Ras and Raf-1. To explain these contrasting findings, it has been suggested that Cot, Ras and Raf-1 may form a multimeric complex that phosphorylates MEK-1. Cot has also been shown to be implicated in T lymphocyte activation.Synonyms: COT, COT proto-oncogene serine/threonine-protein kinase, Cancer Osaka thyroid oncogene, ESTF, MAPK, Mitogen-activated protein kinase kinase kinase 8, Tumor progression locus 2, Tumor progression locus 2
Molecular Weight approx. 53 kDa
Gene ID 1326
NCBI Accession NP_005195
UniProt P41279
Pathways PI3K-Akt Signaling, TCR Signaling

Application Details

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Handling Images back to top
Application Notes ELISA: 1/40000approx. 1/60000. Western Blot: 1/500approx. 1/1000. Immunofluorescence: 1/50approx. 1/200. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-MAP3K8 Antibody Target Details MAP3K8 Application Details Handling back to top
Supplier Images
 image for anti-MAP3K8 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 8) (ABIN265369) anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) antibody
 image for anti-MAP3K8 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 8) (ABIN265369) anti-Mitogen-Activated Protein Kinase Kinase Kinase 8 (MAP3K8) antibody (Image 2)