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|Application / Reactivity||Human|
|Chromatin Immunoprecipitation (ChIP)||1 Antibodies|
|Dot Blot (DB)||2 Antibodies|
|Enzyme Immunoassay (EIA)||8 Antibodies|
|Flow Cytometry (FACS)||6 Antibodies|
|Immunochromatography (IC)||8 Antibodies|
|Immunocytochemistry (ICC)||22 Antibodies|
|Immunofluorescence (IF)||54 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||45 Antibodies|
|Immunohistochemistry (IHC)||115 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||65 Antibodies|
|Immunoprecipitation (IP)||37 Antibodies|
|Intracellular Flow Cytometry (ICFC)||2 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies|
|Mass Cytometry (CyTOF)||2 Antibodies|
|Proximity Ligation Assay (PLA)||3 Antibodies|
|Western Blotting (WB)||226 Antibodies|
|Antigen||Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies|
|Conjugate||This MTOR antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Intracellular Flow Cytometry (ICFC), Mass Cytometry (CyTOF), Immunoprecipitation (IP), Western Blotting (WB)
|6 references available|
|Supplier||Log in to see|
Product Details anti-MTOR AntibodyTarget Details MTOR Application Details Handling References for anti-MTOR Antibody (ABIN2666241) Images
|Purification||The antibody was purified by affinity chromatography.|
Target Details MTORProduct Details anti-MTOR Antibody Application Details Handling References for anti-MTOR Antibody (ABIN2666241) Images back to top
|Alternative Name||mTOR (FRAP1 Antibody Abstract)|
|Background||MTOR (mammalian target of rapamycin) is a Ser/Thr kinase acting as an ATP sensor and is reported to autophosphorylate Ser2481 in the presence of sufficient nutrients. Activated mTOR phosphorylates ribosomal protein S6 kinase 1 (S6K1) and the eukaryotic translation initiation factor 4E binding protein 1 (4EBP1) in response to PI3K/Akt signaling, in turn, promoting cell growth, survival, and proliferation.|
|Research Area||Cardiovascular, Cardiogenesis, Cell Cycle, DNA/RNA|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-MTOR Antibody Target Details MTOR Handling References for anti-MTOR Antibody (ABIN2666241) Images back to top
|Application Notes||Optimal working dilution should be determined by the investigator.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-MTOR Antibody Target Details MTOR Application Details References for anti-MTOR Antibody (ABIN2666241) Images back to top
|Buffer||phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide and 50 % glycerol.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage Comment||Upon receipt, store undiluted at -20°C.|
References for anti-MTOR Antibody (ABIN2666241)Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling Images back to top
Vazquez-Martin, Cufí, Oliveras-Ferraros et al.: "Raptor, a positive regulatory subunit of mTOR complex 1, is a novel phosphoprotein of the rDNA transcription machinery in nucleoli and chromosomal nucleolus organizer regions (NORs)." in: Cell cycle (Georgetown, Tex.), Vol. 10, Issue 18, pp. 3140-52, 2011 (PubMed).
Soliman, Acosta-Jaquez, Dunlop et al.: "mTOR Ser-2481 autophosphorylation monitors mTORC-specific catalytic activity and clarifies rapamycin mechanism of action." in: The Journal of biological chemistry, Vol. 285, Issue 11, pp. 7866-79, 2010 (PubMed).
Nojima, Tokunaga, Eguchi et al.: "The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15461-4, 2003 (PubMed).
Fingar, Salama, Tsou et al.: "Mammalian cell size is controlled by mTOR and its downstream targets S6K1 and 4EBP1/eIF4E." in: Genes & development, Vol. 16, Issue 12, pp. 1472-87, 2002 (PubMed).
Dennis, Jaeschke, Saitoh et al.: "Mammalian TOR: a homeostatic ATP sensor." in: Science (New York, N.Y.), Vol. 294, Issue 5544, pp. 1102-5, 2001 (PubMed).
Brown, Albers, Shin et al.: "A mammalian protein targeted by G1-arresting rapamycin-receptor complex." in: Nature, Vol. 369, Issue 6483, pp. 756-8, 1994 (PubMed).
ImagesProduct Details anti-MTOR Antibody Target Details MTOR Application Details Handling References for anti-MTOR Antibody (ABIN2666241) back to top