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|Antigen||Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies|
|Epitope||AA 2440-2457, pSer2448 Alternatives|
|Conjugate||This MTOR antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|9 references available|
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Product Details anti-MTOR AntibodyTarget Details MTOR Application Details Handling ProductDetails: References for anti-MTOR antibody (ABIN117909) Images
|Specificity||Reactivity occurs with phosphorylated mTOR from Human derived tissues and cells. Reactivity against mTOR from other species has not been determined, however, reactivity with Mouse and Rat is suggested based on protein sequence homologies.|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.|
|Immunogen||A synthetic peptide corresponding to amino acids 2440-2457 of Human mTOR|
Target Details MTORProduct Details anti-MTOR Antibody Application Details Handling ProductDetails: References for anti-MTOR antibody (ABIN117909) Images back to top
|Alternative Name||mTOR / FRAP1 (FRAP1 Antibody Abstract)|
|Background||Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245-kD protein referred to as RAFT1 with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events|
Application DetailsProduct Details anti-MTOR Antibody Target Details MTOR Handling ProductDetails: References for anti-MTOR antibody (ABIN117909) Images back to top
This antibody has been tested for use in Immunohistochemistry (5.0 μg/mL), ELISA(1/15,000-1/65,000) and Western blotting (1/500-1/2,000). Western blotting shows reactivity specific for phospho mTOR detecting a band atapproximately 250 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-MTOR Antibody Target Details MTOR Application Details ProductDetails: References for anti-MTOR antibody (ABIN117909) Images back to top
|Concentration||1.88 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.|
ProductDetails: References for anti-MTOR antibody (ABIN117909)Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling Images back to top
Chase, Rao, Hofmann, Chang-Hasnain: "1550 nm high contrast grating VCSEL." in: Optics express, Vol. 18, Issue 15, pp. 15461-6, 2010
Kristof, Marks-Konczalik, Billings, Moss: "Stimulation of signal transducer and activator of transcription-1 (STAT1)-dependent gene transcription by lipopolysaccharide and interferon-gamma is regulated by mammalian target of rapamycin." in: The Journal of biological chemistry, Vol. 278, Issue 36, pp. 33637-44, 2003
Chen, Zheng, Foster: "Phospholipase D confers rapamycin resistance in human breast cancer cells." in: Oncogene, Vol. 22, Issue 25, pp. 3937-42, 2003
Nojima, Tokunaga, Eguchi, Oshiro, Hidayat, Yoshino, Hara, Tanaka, Avruch, Yonezawa: "The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15461-4, 2003
McMahon, Choi, Lin, Abraham, Lawrence: "The rapamycin-binding domain governs substrate selectivity by the mammalian target of rapamycin." in: Molecular and cellular biology, Vol. 22, Issue 21, pp. 7428-38, 2002
Hudson, Liu, Chiang, Otterness, Loomis, Kaper, Giaccia, Abraham: "Regulation of hypoxia-inducible factor 1alpha expression and function by the mammalian target of rapamycin." in: Molecular and cellular biology, Vol. 22, Issue 20, pp. 7004-14, 2002
Choi, Bertram, Drenan, Carvalho, Zhou, Zheng: "The FKBP12-rapamycin-associated protein (FRAP) is a CLIP-170 kinase." in: EMBO reports, Vol. 3, Issue 10, pp. 988-94, 2002
Inoki, Li, Zhu, Wu, Guan: "TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signalling." in: Nature cell biology, Vol. 4, Issue 9, pp. 648-57, 2002
Zhang, Shu, Hosoi, Murti, Houghton: "Predominant nuclear localization of mammalian target of rapamycin in normal and malignant cells in culture." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 28127-34, 2002
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