You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human MTOR antibody for Enzyme Immunoassay

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • 2610315D21Rik
  • AI327068
  • flat
  • FRAP
  • frap1
  • Frap1
  • FRAP1
  • FRAP2
  • RAFT1
  • RAPT1
  • tor
  • wu:fc22h08
Epitope
AA 2440-2457, pSer2448
65
24
24
23
22
21
13
13
13
10
7
6
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
340
165
151
8
2
2
2
1
1
Host
Rabbit
292
47
2
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
8
7
7
4
4
4
4
4
4
4
4
4
4
3
3
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
221
112
92
62
52
44
32
20
8
7
6
3
2
2
2
2
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN117909
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN358774 EIA IHC (p) WB Rabbit Ig Ser2481 Log in to see Polyclonal 2
0.0008867729 ABIN401320 EIA WB Rabbit IgG AA 2440-2457 Log in to see Polyclonal 6
0.0008867729 ABIN501014 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal 4
0.0008867729 ABIN303334 EIA IF IHC (p) WB Rabbit AA 2440-2457 Log in to see Polyclonal
0.0008867729 ABIN272040 EIA IHC (p) Rabbit Log in to see Polyclonal
0.0008867729 ABIN303048 EIA IHC (p) WB Rabbit pSer2448 Log in to see Polyclonal
0.0008867729 ABIN2726543 EIA IP WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-MTOR antibody for Enzyme Immunoassay

Similar anti-MTOR Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Dot Blot (DB) 2 Antibodies
ELISA 93 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 22 Antibodies
Immunofluorescence (IF) 54 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies
Immunohistochemistry (IHC) 115 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 65 Antibodies
Immunoprecipitation (IP) 37 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Epitope AA 2440-2457, pSer2448
(65), (24), (24), (23), (22), (21), (13), (13), (13), (10), (7), (6), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(340), (165), (151), (8), (2), (2), (2), (1), (1)
Host Rabbit
(292), (47), (2)
Clonality
Polyclonal
Conjugate This MTOR antibody is un-conjugated
(8), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(221), (112), (92), (62), (52), (44), (32), (20), (8), (7), (6), (3), (2), (2), (2), (2), (2), (1)
Pubmed 9 references available
Supplier Log in to see

Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling References for anti-MTOR Antibody (ABIN117909) Images
Specificity Reactivity occurs with phosphorylated mTOR from Human derived tissues and cells. Reactivity against mTOR from other species has not been determined, however, reactivity with Mouse and Rat is suggested based on protein sequence homologies.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.
Immunogen A synthetic peptide corresponding to amino acids 2440-2457 of Human mTOR

Target Details MTOR

Product Details anti-MTOR Antibody Application Details Handling References for anti-MTOR Antibody (ABIN117909) Images back to top
Antigen
Alternative Name mTOR / FRAP1 (FRAP1 Antibody Abstract)
Background Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245-kD protein referred to as RAFT1 with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR
Gene ID 2475
NCBI Accession NP_004949
UniProt P42345
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling References for anti-MTOR Antibody (ABIN117909) Images back to top
Application Notes This antibody has been tested for use in Immunohistochemistry (5.0 μg/mL), ELISA(1/15,000-1/65,000) and Western blotting (1/500-1/2,000). Western blotting shows reactivity specific for phospho mTOR detecting a band atapproximately 250 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details References for anti-MTOR Antibody (ABIN117909) Images back to top
Concentration 1.88 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

References for anti-MTOR Antibody (ABIN117909)

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling Images back to top
Background publications

Chase, Rao, Hofmann, Chang-Hasnain: "1550 nm high contrast grating VCSEL." in: Optics express, Vol. 18, Issue 15, pp. 15461-6, 2010

Kristof, Marks-Konczalik, Billings, Moss: "Stimulation of signal transducer and activator of transcription-1 (STAT1)-dependent gene transcription by lipopolysaccharide and interferon-gamma is regulated by mammalian target of rapamycin." in: The Journal of biological chemistry, Vol. 278, Issue 36, pp. 33637-44, 2003

Chen, Zheng, Foster: "Phospholipase D confers rapamycin resistance in human breast cancer cells." in: Oncogene, Vol. 22, Issue 25, pp. 3937-42, 2003

Nojima, Tokunaga, Eguchi, Oshiro, Hidayat, Yoshino, Hara, Tanaka, Avruch, Yonezawa: "The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15461-4, 2003

McMahon, Choi, Lin, Abraham, Lawrence: "The rapamycin-binding domain governs substrate selectivity by the mammalian target of rapamycin." in: Molecular and cellular biology, Vol. 22, Issue 21, pp. 7428-38, 2002

Hudson, Liu, Chiang, Otterness, Loomis, Kaper, Giaccia, Abraham: "Regulation of hypoxia-inducible factor 1alpha expression and function by the mammalian target of rapamycin." in: Molecular and cellular biology, Vol. 22, Issue 20, pp. 7004-14, 2002

Choi, Bertram, Drenan, Carvalho, Zhou, Zheng: "The FKBP12-rapamycin-associated protein (FRAP) is a CLIP-170 kinase." in: EMBO reports, Vol. 3, Issue 10, pp. 988-94, 2002

Inoki, Li, Zhu, Wu, Guan: "TSC2 is phosphorylated and inhibited by Akt and suppresses mTOR signalling." in: Nature cell biology, Vol. 4, Issue 9, pp. 648-57, 2002

Zhang, Shu, Hosoi, Murti, Houghton: "Predominant nuclear localization of mammalian target of rapamycin in normal and malignant cells in culture." in: The Journal of biological chemistry, Vol. 277, Issue 31, pp. 28127-34, 2002

Images

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling References for anti-MTOR Antibody (ABIN117909) back to top
Supplier Images
 image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2440-2457) (ABIN117909) Immunoblotting. Affinity Purified Anti-mTOR pS 2448 (Rabbit) is shown to detect a 250...
 image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2440-2457) (ABIN117909) Immunoblotting. Metabolic and autocrine regulation of the mTOR pathway by beta-cells.