You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human MTOR antibody for Flow Cytometry

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Reactivity
Human
355
170
165
8
2
2
2
1
1
Host
Mouse
309
44
2
1
Clonality
Monoclonal
Conjugate
This MTOR antibody is un-conjugated
8
7
7
4
4
4
4
4
4
4
4
4
4
4
3
3
3
2
Application
Flow Cytometry (FACS)
229
117
95
62
54
52
37
21
8
8
3
2
2
2
2
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2665280
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2665281 FACS Mouse IgG1 kappa Log in to see 6H9B10 3
1 ABIN2648060 FACS IP Mouse Log in to see F11

Top referenced anti-MTOR antibody for Flow Cytometry

Similar anti-MTOR Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 1 Antibodies
Dot Blot (DB) 2 Antibodies
Electron Microscopy (EM) 1 Antibodies
ELISA 95 Antibodies
Enzyme Immunoassay (EIA) 8 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Immunochromatography (IC) 8 Antibodies
Immunocytochemistry (ICC) 22 Antibodies
Immunofluorescence (IF) 55 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 52 Antibodies
Immunohistochemistry (IHC) 118 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 63 Antibodies
Immunoprecipitation (IP) 37 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Reactivity Human
(355), (170), (165), (8), (2), (2), (2), (1), (1)
Host Mouse
(309), (44), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This MTOR antibody is un-conjugated
(8), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2)
Application Flow Cytometry (FACS)
(229), (117), (95), (62), (54), (52), (37), (21), (8), (8), (3), (2), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling References for anti-MTOR Antibody (ABIN2665280) Images
Cross-Reactivity Mouse (Murine)
Cross-Reactivity (Details) weakly cross-reactive with mouse
Purification The antibody was purified by affinity chromatography.
Clone 6H9B10
Isotype IgG1 kappa

Target Details MTOR

Product Details anti-MTOR Antibody Application Details Handling References for anti-MTOR Antibody (ABIN2665280) Images back to top
Antigen
Alternative Name mTOR (FRAP1 Antibody Abstract)
Background MTOR (mammalian target of rapamycin) is a 289 kD member of the PI3 kinase family, containing HEAT, FRB, and FATC domains. mTOR is expressed in the nucleus and cytoplasm, and is a target for cell-cycle arrest and the immunosuppressive effects of FKBP12-rapamycin complex. mTOR controls ribosome biogenesis and cell growth, and acts as an ATP sensor. mTOR is repressed by nutrient deprivation and mitochondrial uncoupling. mTOR is the target of PI3K-regulated protein kinase, AKT/PKB (phosphorylated at Thr 2446 and Ser2448). mTOR has also been shown to bind RAPTOR, which negatively regulates mTOR kinase activity, and is also inhibited by tuberin-hamartin tumor suppressor complex. The binding of RAPTOR to mTOR is necessary for the mTOR-catalyzed phosphorylation of 4EBP1 in vitro, and it strongly enhances the mTOR kinase activity toward p70-alpha.
Research Area Cardiovascular, Cardiogenesis, Cell Cycle, DNA/RNA
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling References for anti-MTOR Antibody (ABIN2665280) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details References for anti-MTOR Antibody (ABIN2665280) Images back to top
Concentration 0.5 mg/mL
Buffer phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody solution should be stored undiluted between 2°C and 8°C.

References for anti-MTOR Antibody (ABIN2665280)

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling Images back to top
Background publications

Nojima, Tokunaga, Eguchi, Oshiro, Hidayat, Yoshino, Hara, Tanaka, Avruch, Yonezawa: "The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15461-4, 2003

Fingar, Salama, Tsou, Harlow, Blenis: "Mammalian cell size is controlled by mTOR and its downstream targets S6K1 and 4EBP1/eIF4E." in: Genes & development, Vol. 16, Issue 12, pp. 1472-87, 2002

Dennis, Jaeschke, Saitoh, Fowler, Kozma, Thomas: "Mammalian TOR: a homeostatic ATP sensor." in: Science (New York, N.Y.), Vol. 294, Issue 5544, pp. 1102-5, 2001

Images

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling References for anti-MTOR Antibody (ABIN2665280) back to top
Supplier Images
Immunofluorescence (IF) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (ABIN2665280) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) antibody
Western Blotting (WB) image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (ABIN2665280) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) antibody (Image 2)