anti-Human MTOR antibody for Proximity Ligation Assay

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitope
AA 200-250
66
36
28
23
21
15
15
13
12
9
8
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
378
201
197
18
9
8
7
7
7
7
4
2
2
2
1
1
Host
Rabbit
324
49
4
1
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
8
8
7
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Proximity Ligation Assay (PLA), Western Blotting (WB)
252
113
99
75
56
52
36
21
8
7
7
7
2
2
2
2
2
1
1
Supplier
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Catalog No. ABIN151707
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
32.32945 ABIN560949 PLA ELISA Mouse IgG1 kappa AA 1521-1620, partial Log in to see 2C5
1 ABIN1915272 ICC IF IP PLA Rabbit IgG AA 200-250 Log in to see Polyclonal

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Epitope AA 200-250
(66), (36), (28), (23), (21), (15), (15), (13), (12), (9), (8), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(378), (201), (197), (18), (9), (8), (7), (7), (7), (7), (4), (2), (2), (2), (1), (1)
Host Rabbit
(324), (49), (4), (1)
Clonality
Polyclonal
Conjugate This MTOR antibody is un-conjugated
(8), (8), (7), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Proximity Ligation Assay (PLA), Western Blotting (WB)
(252), (113), (99), (75), (56), (52), (36), (21), (8), (7), (7), (7), (2), (2), (2), (2), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling References for anti-MTOR antibody (ABIN151707) Images
Purification Immunogen affinity purified
Immunogen The immunogen recognized by this antibody maps to a region between residue 200 and 250 of human Mammalian Target of Rapamycin using the numbering given in entry NP_004949.1 (GeneID 2475).

Target Details MTOR

Product Details anti-MTOR Antibody Application Details Handling References for anti-MTOR antibody (ABIN151707) Images back to top
Antigen
Alternative Name MTOR / FRAP1 (FRAP1 Antibody Abstract)
Background Gene Symbol: MTOR
Gene ID 2475
NCBI Accession NP_004949
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling References for anti-MTOR antibody (ABIN151707) Images back to top
Application Notes Western Blot 1:2000 - 1:10000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 2- 10 μg/mg lysate, Proximity Ligation Assay 1:1000-1:20000
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details References for anti-MTOR antibody (ABIN151707) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

References for anti-MTOR antibody (ABIN151707)

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling Images back to top
Product cited in:

Suzuki, Roa, Kawamoto, Ishige, Wistuba, Li, Thomas, Shoda: "Expression of insulin-like growth factor I receptor as a biomarker for predicting prognosis in biliary tract cancer patients." in: Molecular and clinical oncology, Vol. 3, Issue 3, pp. 464-470, 2015 Further details: Immunohistochemistry

Liu, Yang: "MTDH and EphA7 are markers for metastasis and poor prognosis of gallbladder adenocarcinoma." in: Diagnostic cytopathology, Vol. 41, Issue 3, pp. 199-205, 2013 (Sample species: Human). Further details: Immunohistochemistry

Lionello, Blandamura, Loreggian, Ottaviano, Giacomelli, Marchese-Ragona, Velardita, Staffieri, Marioni: "High mTOR expression is associated with a worse oncological outcome in laryngeal carcinoma treated with postoperative radiotherapy: a pilot study." in: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, Vol. 41, Issue 2, pp. 136-40, 2012

Bolster, Vary, Kimball, Jefferson: "Leucine regulates translation initiation in rat skeletal muscle via enhanced eIF4G phosphorylation." in: The Journal of nutrition, Vol. 134, Issue 7, pp. 1704-10, 2004

Images

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling References for anti-MTOR antibody (ABIN151707) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 200-250) antibody (ABIN151707) Immunofluorescence detection of mTor (red), using ABIN151707: Sample: L6 myotubes obs...
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 200-250) antibody (ABIN151707) Immunoprecipitation of mTOR for use in Kinase Assays. Sample: Recombinant 4E-BP1 was...
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 200-250) antibody (ABIN151707) Western Blot: TOR/mTOR Antibody [ABIN151707] - Whole cell lysate from HeLa (5, 15 and...
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 200-250) antibody (ABIN151707) Western Blot: TOR/mTOR Antibody [ABIN151707] - Whole cell lysate (50, 15, 5 ug) from ...
Proximity Ligation Assay (PLA) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 200-250) antibody (ABIN151707) Proximity Ligation Assay: TOR/mTOR Antibody [ABIN151707] - Secondary-conjugate Duolin...
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 200-250) antibody (ABIN151707) Western Blot: TOR/mTOR Antibody - Detection of Human mTOR by Western Blot. Samples: ...