anti-Rat (Rattus) MTOR antibody for Western Blotting

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitope
AA 2415-2464, pSer2448
65
36
28
23
21
15
15
13
12
10
8
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
378
200
197
18
9
8
7
7
7
7
4
2
2
2
1
1
Host
Rabbit
324
49
4
1
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
8
8
7
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
252
113
98
75
57
52
37
22
8
7
7
7
3
2
2
2
2
1
1
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Catalog No. ABIN1531910
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1469584 ABIN1531715 ELISA WB Rabbit IgG AA 2412-2461, pThr2446 Log in to see Polyclonal 3
3.1469584 ABIN1532744 ELISA WB Rabbit IgG AA 2412-2461 Log in to see Polyclonal 3
3.1469584 ABIN153493 ELISA EM IHC IHC (p) WB Rabbit IgG pSer2448 Log in to see Polyclonal 4
3.1469584 ABIN151707 ICC IF IP IHC PLA WB Rabbit AA 200-250 Log in to see Polyclonal 4
3.1469584 ABIN1532942 IHC ELISA WB Rabbit IgG AA 2415-2464 Log in to see Polyclonal
3.1469584 ABIN1532464 IHC ELISA WB Rabbit IgG AA 2447-2496 Log in to see Polyclonal
3.1469584 ABIN968384 IF WB Mouse IgG1 AA 185-290 Log in to see 30-RAFT1 5
3.1469584 ABIN968383 IF WB Mouse IgG1 AA 185-290 Log in to see 30-RAFT1 5
3.1469584 ABIN447030 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal 1
3.1469584 ABIN3185709 ELISA IF IHC WB Rabbit IgG Ser396 Log in to see Polyclonal
3.1469584 ABIN3182280 ELISA WB Rabbit IgG pThr2446 Log in to see Polyclonal
3.1469584 ABIN3182075 ELISA IF IHC WB Rabbit IgG pSer2448 Log in to see Polyclonal
3.1469584 ABIN3185707 ELISA IHC WB Rabbit IgG Ser392 Log in to see Polyclonal
3.1469584 ABIN3185708 ELISA IHC WB Rabbit IgG Ser394 Log in to see Polyclonal
3.1469584 ABIN2855089 WB Rabbit IgG Internal Region Log in to see Polyclonal
3.1469584 ABIN2957676 WB Rabbit C-Term, pSer2446 Log in to see Polyclonal
3.1469584 ABIN498698 IHC (p) WB Rabbit pSer2448 Log in to see Polyclonal
3.1469584 ABIN2705013 IC IF WB Rabbit C-Term Log in to see Polyclonal
3.1469584 ABIN498610 IF IHC (p) WB Rabbit Log in to see Polyclonal
3.1469584 ABIN2855088 ChIP ICC IF IP IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Epitope AA 2415-2464, pSer2448
(65), (36), (28), (23), (21), (15), (15), (13), (12), (10), (8), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(378), (200), (197), (18), (9), (8), (7), (7), (7), (7), (4), (2), (2), (2), (1), (1)
Host Rabbit
(324), (49), (4), (1)
Clonality
Polyclonal
Conjugate This MTOR antibody is un-conjugated
(8), (8), (7), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(252), (113), (98), (75), (57), (52), (37), (22), (8), (7), (7), (7), (3), (2), (2), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling References for anti-MTOR antibody (ABIN1531910) Images
Specificity

mTOR (Phospho-Ser2448) Antibody detects endogenous levels of mTOR only when phosphorylated at Ser2448.

PhosphorylationH:S2448 M:S2448 R:S2448

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human mTOR around the phosphorylation site of Ser2448.
Isotype IgG

Target Details MTOR

Product Details anti-MTOR Antibody Application Details Handling References for anti-MTOR antibody (ABIN1531910) Images back to top
Antigen
Alternative Name mTOR (FRAP1 Antibody Abstract)
Background

Synonyms: FRAP, FRAP1, FRAP2, RAFT1, Rapamycin target protein, kinase mTOR

NCBI Gene Symbol: MTOR

Molecular Weight 288 kDa
Gene ID 2475
OMIM 601231
UniProt P42345
Research Area Cardiovascular, Cardiogenesis, Cell Cycle, DNA/RNA
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling References for anti-MTOR antibody (ABIN1531910) Images back to top
Application Notes WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:40000
Comment

Unigene-Number: Hs.338207 (NCBI Gene Symbol: MTOR)

Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details References for anti-MTOR antibody (ABIN1531910) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20°C for at least 1 year.
Expiry Date 12 months

References for anti-MTOR antibody (ABIN1531910)

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling Images back to top
Product cited in:

Luo, Yoneda, Ohmori, Sasaki, Shimbo, Eto, Kato, Miyano, Kobayashi, Sasahira, Chihara, Kuniyasu: "Cancer usurps skeletal muscle as an energy repository." in: Cancer research, Vol. 74, Issue 1, pp. 330-40, 2014

Images

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling References for anti-MTOR antibody (ABIN1531910) back to top
Supplier Images
Western Blotting (WB) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448), (AA 2415-2464) antibody (ABIN1531910) Western blot analysis of extracts from HeLa cells treated with EGF 200ng/ml 30', usin...
Immunohistochemistry (IHC) image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448), (AA 2415-2464) antibody (ABIN1531910) Immunohistochemistry analysis of paraffin-embedded human brain, using mTOR (Phospho-S...