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anti-Zebrafish (Danio rerio) MTOR antibody for Western Blotting

Recommended MTOR Antibody (supplied by: Log in to see )

Antigen
Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
  • 2610315D21Rik
  • AI327068
  • flat
  • FRAP
  • frap1
  • Frap1
  • FRAP1
  • FRAP2
  • RAFT1
  • RAPT1
  • tor
  • wu:fc22h08
Epitope
AA 2440-2457
66
24
24
23
22
21
13
13
13
10
7
6
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Zebrafish (Danio rerio)
340
164
151
8
2
1
1
1
1
Host
Rabbit
292
47
2
Clonality
Polyclonal
Conjugate
This MTOR antibody is un-conjugated
8
7
7
4
4
4
4
4
4
4
4
4
4
3
3
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
221
112
92
63
52
44
32
20
8
7
6
3
2
2
2
2
2
1
Supplier
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Catalog No. ABIN401320
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0011758144 ABIN303334 EIA IF IHC (p) WB Rabbit AA 2440-2457 Log in to see Polyclonal

Top referenced anti-MTOR antibody for Western Blotting

Similar anti-MTOR Antibodies

Application / Reactivity Human Mouse (Murine) Dog (Canine) Zebrafish (Danio rerio)
Western Blotting (WB) 225 Antibodies 85 Antibodies 2 Antibodies 2 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Mass Cytometry (CyTOF) 2 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies
Intracellular Flow Cytometry (ICFC) 2 Antibodies
Immunoprecipitation (IP) 37 Antibodies 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 65 Antibodies 31 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 115 Antibodies

General

Antigen Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) Antibodies
Epitope AA 2440-2457
(66), (24), (24), (23), (22), (21), (13), (13), (13), (10), (7), (6), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Human, Mouse (Murine), Zebrafish (Danio rerio)
(340), (164), (151), (8), (2), (1), (1), (1), (1)
Host Rabbit
(292), (47), (2)
Clonality
Polyclonal
Conjugate This MTOR antibody is un-conjugated
(8), (7), (7), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(221), (112), (92), (63), (52), (44), (32), (20), (8), (7), (6), (3), (2), (2), (2), (2), (2), (1)
Pubmed 6 references available
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Product Details anti-MTOR Antibody

Target Details MTOR Application Details Handling References for anti-MTOR Antibody (ABIN401320) Images
Specificity Reactivity occurs with both phosphorylated and non-phosphorylated forms of mTOR at S2448 from derived tissues and cells.
Cross-Reactivity (Details) Species reactivity (expected):Rat, Mouse, Zebrafish, Canine.
Species reactivity (tested):Human.
Purification Immunoaffinity chromatography
Immunogen Synthetic peptide corresponding to amino acids 2440-2457 of human mTOR
Isotype IgG

Target Details MTOR

Product Details anti-MTOR Antibody Application Details Handling References for anti-MTOR Antibody (ABIN401320) Images back to top
Antigen
Alternative Name mTOR / FRAP1 (FRAP1 Antibody Abstract)
Background Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR has a long list of synonyms including FK506 binding protein12 - rapamycin associated protein 1, FK506 binding protein12 - rapamycin associated protein 2, FRAP1, FRAP2, RAFT1, RAPT1 and/or FKBP12-rapamycin associated protein (FRAP). mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245-kD protein referred to as RAFT1 with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR
Gene ID 2475
NCBI Accession NP_004949
UniProt P42345
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-MTOR Antibody Target Details MTOR Handling References for anti-MTOR Antibody (ABIN401320) Images back to top
Application Notes ELISA: 1/4000 - 1/20000. Western Blot: 1/250 - 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-MTOR Antibody Target Details MTOR Application Details References for anti-MTOR Antibody (ABIN401320) Images back to top
Concentration 0.40 mg/mL (by UV absorbance at 280 nm)
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C

References for anti-MTOR Antibody (ABIN401320)

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling Images back to top
Background publications

Chase, Rao, Hofmann, Chang-Hasnain: "1550 nm high contrast grating VCSEL." in: Optics express, Vol. 18, Issue 15, pp. 15461-6, 2010

Kristof, Marks-Konczalik, Billings, Moss: "Stimulation of signal transducer and activator of transcription-1 (STAT1)-dependent gene transcription by lipopolysaccharide and interferon-gamma is regulated by mammalian target of rapamycin." in: The Journal of biological chemistry, Vol. 278, Issue 36, pp. 33637-44, 2003

Nojima, Tokunaga, Eguchi, Oshiro, Hidayat, Yoshino, Hara, Tanaka, Avruch, Yonezawa: "The mammalian target of rapamycin (mTOR) partner, raptor, binds the mTOR substrates p70 S6 kinase and 4E-BP1 through their TOR signaling (TOS) motif." in: The Journal of biological chemistry, Vol. 278, Issue 18, pp. 15461-4, 2003

McMahon, Choi, Lin, Abraham, Lawrence: "The rapamycin-binding domain governs substrate selectivity by the mammalian target of rapamycin." in: Molecular and cellular biology, Vol. 22, Issue 21, pp. 7428-38, 2002

Hudson, Liu, Chiang, Otterness, Loomis, Kaper, Giaccia, Abraham: "Regulation of hypoxia-inducible factor 1alpha expression and function by the mammalian target of rapamycin." in: Molecular and cellular biology, Vol. 22, Issue 20, pp. 7004-14, 2002

Choi, Bertram, Drenan, Carvalho, Zhou, Zheng: "The FKBP12-rapamycin-associated protein (FRAP) is a CLIP-170 kinase." in: EMBO reports, Vol. 3, Issue 10, pp. 988-94, 2002

Images

Product Details anti-MTOR Antibody Target Details MTOR Application Details Handling References for anti-MTOR Antibody (ABIN401320) back to top
Supplier Images
 image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2440-2457) (ABIN401320) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2440-2457) antibody
 image for anti-MTOR antibody (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (AA 2440-2457) (ABIN401320) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (AA 2440-2457) antibody (Image 2)