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anti-Human NCF1 antibody for Immunohistochemistry

Recommended NCF1 Antibody (supplied by: Log in to see )

Antigen
Neutrophil Cytosol Factor 1 (NCF1) Antibodies
  • NCF1A
  • NOXO2
  • SH3PXD1A
  • p47phox
  • Ncf-1
  • p47
  • neutrophil cytosolic factor 1
  • NCF1
  • Ncf1
Epitope
pSer328
49
24
15
14
10
9
9
8
5
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
190
87
80
14
11
10
9
7
5
1
1
Host
Rabbit
168
35
2
Clonality
Polyclonal
Conjugate
This NCF1 antibody is un-conjugated
6
6
6
4
4
4
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), ELISA
162
100
91
38
28
27
9
8
6
5
2
Options
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Catalog No. ABIN1531718
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.145143 ABIN1532163 IHC ELISA WB Rabbit IgG pSer345 Log in to see Polyclonal 1
9.145143 ABIN1531723 IF IHC ELISA WB Rabbit IgG pSer359 Log in to see Polyclonal 1
9.145143 ABIN1532747 IF IHC ELISA Rabbit IgG Log in to see Polyclonal 1
9.145143 ABIN1532752 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
9.145143 ABIN1533187 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
9.145143 ABIN1532751 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
9.145143 ABIN2957702 IHC WB Rabbit C-Term Log in to see Polyclonal
9.145143 ABIN2801294 IHC WB Rabbit C-Term, pSer345 Log in to see Polyclonal
9.145143 ABIN2705083 IHC WB Rabbit C-Term, pSer304 Log in to see Polyclonal
9.145143 ABIN2705084 IHC WB Rabbit C-Term, pSer328 Log in to see Polyclonal
9.145143 ABIN2705087 IP IHC WB Rabbit C-Term Log in to see Polyclonal
9.145143 ABIN2705085 IC IF IHC WB Rabbit C-Term, pSer359 Log in to see Polyclonal
9.145143 ABIN3222956 IC IF IHC WB Rabbit C-Term Log in to see Polyclonal
9.145143 ABIN1848360 IHC ELISA WB Rabbit C-Term, Ser345 Log in to see Polyclonal
9.145143 ABIN1847806 IHC ELISA WB Rabbit C-Term, pSer345 Log in to see Polyclonal
1 ABIN1531722 IHC ELISA Rabbit IgG pSer370 Log in to see Polyclonal 2
1 ABIN3182821 ELISA IF IHC WB Rabbit IgG pSer359 Log in to see Polyclonal
1 ABIN3186265 ELISA IHC WB Rabbit IgG Tyr905 Log in to see Polyclonal
1 ABIN3186264 ELISA IF IHC WB Rabbit IgG Tyr9 Log in to see Polyclonal
1 ABIN3182707 ELISA IHC WB Rabbit IgG pSer345 Log in to see Polyclonal

Top referenced anti-NCF1 antibody for Immunohistochemistry

  • Mouse (Murine) Polyclonal NCF1 Primary Antibody for IHC, ELISA - ABIN1531722 (2 Publications): Lee, Ko, Jeong, Park, Kim: β-Lapachone suppresses neuroinflammation by modulating the expression of cytokines and matrix metalloproteinases in activated microglia. in Journal of neuroinflammation 2015 (PubMed)

  • Human Polyclonal NCF1 Primary Antibody for IHC, ELISA - ABIN1531718 (2 Publications): Kröller-Schön, Steven, Kossmann, Scholz, Daub, Oelze, Xia, Hausding, Mikhed, Zinßius, Mader, Stamm, Treiber, Scharffetter-Kochanek, Li, Schulz, Wenzel, Münzel, Daiber: Molecular mechanisms of the crosstalk between mitochondria and NADPH oxidase through reactive oxygen species - studies in white blood cells and in animal models. in Antioxidants & redox signaling 2013 (PubMed)

Similar anti-NCF1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies 2 Antibodies 1 Antibodies
Western Blotting (WB) 147 Antibodies 64 Antibodies 45 Antibodies
Immunoprecipitation (IP) 9 Antibodies 4 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 38 Antibodies 16 Antibodies 18 Antibodies
Immunohistochemistry (IHC) 87 Antibodies 36 Antibodies 29 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 27 Antibodies 14 Antibodies 27 Antibodies
Immunofluorescence (IF) 28 Antibodies

General

Antigen Neutrophil Cytosol Factor 1 (NCF1) Antibodies
Epitope pSer328
(49), (24), (15), (14), (10), (9), (9), (8), (5), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(190), (87), (80), (14), (11), (10), (9), (7), (5), (1), (1)
Host Rabbit
(168), (35), (2)
Clonality
Polyclonal
Conjugate This NCF1 antibody is un-conjugated
(6), (6), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), ELISA
(162), (100), (91), (38), (28), (27), (9), (8), (6), (5), (2)
Pubmed 2 references available
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Product Details anti-NCF1 Antibody

Target Details NCF1 Application Details Handling References for anti-NCF1 Antibody (ABIN1531718) Images
Specificity Neutrophil Cytosol Factor 1 (Phospho-Ser328) Antibody detects endogenous levels of Neutrophil Cytosol Factor 1 only when phosphorylated at Ser328.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of Ser328.
Isotype IgG

Target Details NCF1

Product Details anti-NCF1 Antibody Application Details Handling References for anti-NCF1 Antibody (ABIN1531718) Images back to top
Antigen
Alternative Name Neutrophil Cytosol Factor 1 (NCF1 Antibody Abstract)
Background Molecular weight of target protein: 44 kDa
Synonyms: 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NCF1, Neutrophil cytosol factor 1, Neutrophil cytosolic factor 1, Neutrophil NADPH oxidase factor 1, P47 phox, p47-phox
Molecular Weight 44 kDa
Gene ID 653361
OMIM 233700
UniProt P14598
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-NCF1 Antibody Target Details NCF1 Handling References for anti-NCF1 Antibody (ABIN1531718) Images back to top
Application Notes IHC: 1:50-1:100
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.647047, Hs.655201 (NCBI Gene Symbol: NCF1)

Restrictions For Research Use only

Handling

Product Details anti-NCF1 Antibody Target Details NCF1 Application Details References for anti-NCF1 Antibody (ABIN1531718) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-NCF1 Antibody (ABIN1531718)

Product Details anti-NCF1 Antibody Target Details NCF1 Application Details Handling Images back to top
Product cited in:

Kröller-Schön, Steven, Kossmann, Scholz, Daub, Oelze, Xia, Hausding, Mikhed, Zinßius, Mader, Stamm, Treiber, Scharffetter-Kochanek, Li, Schulz, Wenzel, Münzel, Daiber: "Molecular mechanisms of the crosstalk between mitochondria and NADPH oxidase through reactive oxygen species - studies in white blood cells and in animal models." in: Antioxidants & redox signaling, 2013 Method employed by authors: Western Blotting (WB) (1:500). Further details: Total aortic homogenates were blotted and stained for p47phox Ser328 phosphorylation using a specific polyclonal rabbit antibody from antibodies-online (Atlanta, GA, USA) at a dilution of 1:500

Background publications

Lomax, Leto, Nunoi, Gallin, Malech: "Recombinant 47-kilodalton cytosol factor restores NADPH oxidase in chronic granulomatous disease." in: Science (New York, N.Y.), Vol. 245, Issue 4916, pp. 409-12, 1989

Images

Product Details anti-NCF1 Antibody Target Details NCF1 Application Details Handling References for anti-NCF1 Antibody (ABIN1531718) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-NCF1 antibody (Neutrophil Cytosol Factor 1) (pSer328) (ABIN1531718) Immunohistochemistry analysis of paraffin-embedded human tonsil, using Neutrophil Cyt...