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anti-Mouse (Murine) NCF1 antibody for ELISA

Recommended NCF1 Antibody (supplied by: Log in to see )

Antigen
Neutrophil Cytosol Factor 1 (NCF1) Antibodies
  • Ncf-1
  • NCF1A
  • NOXO2
  • p47
  • p47phox
  • SH3PXD1A
Epitope
pSer328
56
23
15
15
13
12
11
8
6
5
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
193
73
67
13
10
9
7
6
5
4
2
1
Host
Rabbit
179
34
2
Clonality
Polyclonal
Conjugate
This NCF1 antibody is un-conjugated
6
6
6
4
4
4
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), ELISA
166
114
100
30
26
23
12
5
4
2
1
Supplier
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Catalog No. ABIN1531718
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012155735 ABIN1531722 IHC ELISA Rabbit IgG pSer370 Log in to see Polyclonal 2
0.0012155735 ABIN1532751 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.0012155735 ABIN1532747 IF IHC ELISA Rabbit IgG Log in to see Polyclonal 1
0.0012155735 ABIN2449051 IHC ELISA WB Rabbit IgG Ser370 Log in to see Polyclonal
0.0012155735 ABIN2449050 IF IHC ELISA Rabbit IgG Log in to see Polyclonal
0.0012155735 ABIN1586076 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0012155735 ABIN1456707 IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0012155735 ABIN790886 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012155735 ABIN1737778 IHC (p) ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0012155735 ABIN1834023 ELISA WB Goat Internal Region Log in to see Polyclonal
0.0012155735 ABIN3186265 ELISA IHC WB Rabbit IgG Tyr905 Log in to see Polyclonal
0.0012155735 ABIN3182894 ELISA IHC WB Rabbit IgG pSer370 Log in to see Polyclonal
0.0012155735 ABIN3182892 ELISA IHC Rabbit IgG pSer328 Log in to see Polyclonal
0.0012155735 ABIN3186267 ELISA IF IHC Rabbit IgG Tyr931 Log in to see Polyclonal
0.0012155735 ABIN1737777 IHC (p) ELISA WB Goat C-Term Log in to see Polyclonal
0.0012155735 ABIN2467105 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012155735 ABIN2317928 ELISA WB APC Goat Log in to see Polyclonal
0.0012155735 ABIN2317926 ELISA WB Alkaline Phosphatase (AP) Goat Log in to see Polyclonal
0.0012155735 ABIN2317931 ELISA WB Biotin Goat Log in to see Polyclonal
0.0012155735 ABIN2317934 ELISA WB FITC Goat Log in to see Polyclonal

Top referenced anti-NCF1 antibody for ELISA

Similar anti-NCF1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 149 Antibodies 54 Antibodies 39 Antibodies
Immunoprecipitation (IP) 4 Antibodies 1 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies 11 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 97 Antibodies 35 Antibodies 28 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies 12 Antibodies 23 Antibodies
Immunofluorescence (IF) 30 Antibodies 8 Antibodies 5 Antibodies
Immunocytochemistry (ICC) 12 Antibodies

General

Antigen Neutrophil Cytosol Factor 1 (NCF1) Antibodies
Epitope pSer328
(56), (23), (15), (15), (13), (12), (11), (8), (6), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(193), (73), (67), (13), (10), (9), (7), (6), (5), (4), (2), (1)
Host Rabbit
(179), (34), (2)
Clonality
Polyclonal
Conjugate This NCF1 antibody is un-conjugated
(6), (6), (6), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), ELISA
(166), (114), (100), (30), (26), (23), (12), (5), (4), (2), (1)
Pubmed 2 references available
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Product Details anti-NCF1 Antibody

Target Details NCF1 Application Details Handling References for anti-NCF1 Antibody (ABIN1531718) Images
Specificity Neutrophil Cytosol Factor 1 (Phospho-Ser328) Antibody detects endogenous levels of Neutrophil Cytosol Factor 1 only when phosphorylated at Ser328.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of Ser328.
Isotype IgG

Target Details NCF1

Product Details anti-NCF1 Antibody Application Details Handling References for anti-NCF1 Antibody (ABIN1531718) Images back to top
Antigen
Alternative Name Neutrophil Cytosol Factor 1 (NCF1 Antibody Abstract)
Background Molecular weight of target protein: 44 kDa
Synonyms: 47 kDa autosomal chronic granulomatous disease protein, 47 kDa neutrophil oxidase factor, NCF-1, NCF-47K, NCF1, Neutrophil cytosol factor 1, Neutrophil cytosolic factor 1, Neutrophil NADPH oxidase factor 1, P47 phox, p47-phox
Molecular Weight 44 kDa
Gene ID 653361
OMIM 233700
UniProt P14598
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-NCF1 Antibody Target Details NCF1 Handling References for anti-NCF1 Antibody (ABIN1531718) Images back to top
Application Notes IHC: 1:50-1:100
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.647047, Hs.655201 (NCBI Gene Symbol: NCF1)

Restrictions For Research Use only

Handling

Product Details anti-NCF1 Antibody Target Details NCF1 Application Details References for anti-NCF1 Antibody (ABIN1531718) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-NCF1 Antibody (ABIN1531718)

Product Details anti-NCF1 Antibody Target Details NCF1 Application Details Handling Images back to top
Product cited in:

Kröller-Schön, Steven, Kossmann, Scholz, Daub, Oelze, Xia, Hausding, Mikhed, Zinßius, Mader, Stamm, Treiber, Scharffetter-Kochanek, Li, Schulz, Wenzel, Münzel, Daiber: "Molecular mechanisms of the crosstalk between mitochondria and NADPH oxidase through reactive oxygen species - studies in white blood cells and in animal models." in: Antioxidants & redox signaling, 2013 Method employed by authors: Western Blotting(WB) (1:500). Further details: Total aortic homogenates were blotted and stained for p47phox Ser328 phosphorylation using a specific polyclonal rabbit antibody from antibodies-online (Atlanta, GA, USA) at a dilution of 1:500

Background publications

Lomax, Leto, Nunoi, Gallin, Malech: "Recombinant 47-kilodalton cytosol factor restores NADPH oxidase in chronic granulomatous disease." in: Science (New York, N.Y.), Vol. 245, Issue 4916, pp. 409-12, 1989

Images

Product Details anti-NCF1 Antibody Target Details NCF1 Application Details Handling References for anti-NCF1 Antibody (ABIN1531718) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-NCF1 antibody (Neutrophil Cytosol Factor 1) (pSer328) (ABIN1531718) Immunohistochemistry analysis of paraffin-embedded human tonsil, using Neutrophil Cyt...