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anti-Human PDK2 antibody for Enzyme Immunoassay

Recommended PDK2 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) Antibodies
  • 3j828
  • MGC52849
  • MGC132353
  • PDHK2
  • Pdk
  • PDK2
  • PDKII
  • zgc:56209
Epitope
C-Term
20
17
16
15
8
5
5
3
3
2
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
122
60
32
5
3
1
Host
Rabbit
104
38
Clonality
Polyclonal
Conjugate
This PDK2 antibody is un-conjugated
9
9
8
7
7
7
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
121
84
62
27
17
11
5
2
1
1
1
1
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Catalog No. ABIN359096
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008870071 ABIN781865 EIA IHC (p) WB Mouse IgG2a Log in to see 2G1
0.0008870071 ABIN781866 EIA IHC (p) WB Mouse IgG2a Log in to see 5F8

Top referenced anti-PDK2 antibody for Enzyme Immunoassay

Similar anti-PDK2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine) Human
ELISA 10 Antibodies 22 Antibodies 73 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 7 Antibodies 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies 10 Antibodies
Western Blotting (WB) 21 Antibodies 43 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) Antibodies
Epitope C-Term
(20), (17), (16), (15), (8), (5), (5), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(122), (60), (32), (5), (3), (1)
Host Rabbit
(104), (38)
Clonality
Polyclonal
Conjugate This PDK2 antibody is un-conjugated
(9), (9), (8), (7), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(121), (84), (62), (27), (17), (11), (5), (2), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-PDK2 Antibody

Target Details PDK2 Application Details Handling References for anti-PDK2 Antibody (ABIN359096) Images
Specificity This antibody reacts to PDK2.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-term region of human PDK2.
Isotype Ig

Target Details PDK2

Product Details anti-PDK2 Antibody Application Details Handling References for anti-PDK2 Antibody (ABIN359096) Images back to top
Antigen
Alternative Name PDK2 (PDK2 Antibody Abstract)
Background PDK2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.Synonyms: Pyruvate dehydrogenase kinase isoform 2, Pyruvate dehydrogenase lipoamide kinase isozyme 2 mitochondrial
Gene ID 5164, 9606
UniProt Q15119
Research Area Cancer, Metabolism
Pathways PI3K-Akt Signaling, RTK Signaling

Application Details

Product Details anti-PDK2 Antibody Target Details PDK2 Handling References for anti-PDK2 Antibody (ABIN359096) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PDK2 Antibody Target Details PDK2 Application Details References for anti-PDK2 Antibody (ABIN359096) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PDK2 Antibody (ABIN359096)

Product Details anti-PDK2 Antibody Target Details PDK2 Application Details Handling Images back to top
Background publications

Sun, Zhou, Chang, McFate, Verma, Califano: "Mitochondrial mutations contribute to HIF1alpha accumulation via increased reactive oxygen species and up-regulated pyruvate dehydrogenease kinase 2 in head and neck squamous cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 2, pp. 476-84, 2009

Richards, Sardella, Schulte: "Regulation of pyruvate dehydrogenase in the common killifish, Fundulus heteroclitus, during hypoxia exposure." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 295, Issue 3, pp. R979-90, 2008

Zhang, Ma, Sadana, Chowdhury, Gaillard, Wang, McDonnell, Unterman, Elam, Park: "Estrogen-related receptors stimulate pyruvate dehydrogenase kinase isoform 4 gene expression." in: The Journal of biological chemistry, Vol. 281, Issue 52, pp. 39897-906, 2006

Images

Product Details anti-PDK2 Antibody Target Details PDK2 Application Details Handling References for anti-PDK2 Antibody (ABIN359096) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PDK2 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 2) (C-Term) (ABIN359096) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-PDK2 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 2) (C-Term) (ABIN359096) (LEFT) Western blot analysis of PDK2 Antibody (C-term) in mouse intestine tissue lysa...