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anti-Human PDK2 antibody for Flow Cytometry

Recommended PDK2 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) Antibodies
  • 3j828
  • MGC52849
  • MGC132353
  • PDHK2
  • Pdk
  • PDK2
  • PDKII
  • zgc:56209
Reactivity
Human
122
61
33
5
3
1
Host
Rabbit
104
38
Clonality
Polyclonal
Conjugate
This PDK2 antibody is un-conjugated
9
9
8
7
7
7
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
121
84
62
27
16
11
5
3
1
1
1
1
Supplier
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Catalog No. ABIN954072
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN653784 FACS IHC (p) WB Rabbit Ig Log in to see Polyclonal 2
0.30226976 ABIN2496010 FACS WB Rabbit Ig Fraction Log in to see Polyclonal
0.0010482287 ABIN1953472 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal
0.0010482287 ABIN1956760 FACS IHC ELISA WB FITC Rabbit IgG Log in to see Polyclonal
0.0010482287 ABIN1955556 FACS IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.0010482287 ABIN1954340 FACS IHC ELISA WB APC Rabbit IgG Log in to see Polyclonal
0.0010482287 ABIN1959100 FACS IHC ELISA WB PE Rabbit IgG Log in to see Polyclonal
0.0010482287 ABIN1957876 FACS IHC ELISA WB HRP Rabbit IgG Log in to see Polyclonal
0.0010482287 ABIN1810910 FACS IHC (p) WB Rabbit Log in to see Polyclonal
0.0010482287 ABIN2332607 FACS IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0010482287 ABIN2332627 FACS IHC ELISA WB APC Rabbit IgG Log in to see Polyclonal
0.0010482287 ABIN2332629 FACS IHC ELISA WB FITC Rabbit IgG Log in to see Polyclonal
0.0010482287 ABIN2332633 FACS IHC ELISA WB PE Rabbit IgG Log in to see Polyclonal
0.0010482287 ABIN2332626 FACS IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Log in to see Polyclonal
0.0010482287 ABIN2332628 FACS IHC ELISA WB Biotin Rabbit IgG Log in to see Polyclonal
0.0010482287 ABIN2793804 FACS IHC WB Rabbit Ig Fraction Log in to see Polyclonal

Top referenced anti-PDK2 antibody for Flow Cytometry

Similar anti-PDK2 Antibodies

Application / Reactivity Human
ELISA 73 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (IF) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 61 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
RNA Interference (RNAi) 1 Antibodies
Western Blotting (WB) 107 Antibodies

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) Antibodies
Reactivity Human
(122), (61), (33), (5), (3), (1)
Host Rabbit
(104), (38)
Clonality
Polyclonal
Conjugate This PDK2 antibody is un-conjugated
(9), (9), (8), (7), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(121), (84), (62), (27), (16), (11), (5), (3), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-PDK2 Antibody

Target Details PDK2 Application Details Handling References for anti-PDK2 Antibody (ABIN954072) Images
Specificity This antibody recognizes Human PDK2.
Purification Protein A column, followed by protein affinity purification
Immunogen Recombinant Human PDK2 protein.
Isotype Ig

Target Details PDK2

Product Details anti-PDK2 Antibody Application Details Handling References for anti-PDK2 Antibody (ABIN954072) Images back to top
Antigen
Alternative Name PDK2 (PDK2 Antibody Abstract)
Background PDK2 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.Synonyms: Pyruvate dehydrogenase kinase isoform 2, Pyruvate dehydrogenase lipoamide kinase isozyme 2 mitochondrial
Molecular Weight 46154 Da
Gene ID 5164
NCBI Accession NP_001186828
Research Area Cancer, Metabolism
Pathways PI3K-Akt Signaling, RTK Signaling

Application Details

Product Details anti-PDK2 Antibody Target Details PDK2 Handling References for anti-PDK2 Antibody (ABIN954072) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-PDK2 Antibody Target Details PDK2 Application Details References for anti-PDK2 Antibody (ABIN954072) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-PDK2 Antibody (ABIN954072)

Product Details anti-PDK2 Antibody Target Details PDK2 Application Details Handling Images back to top
Background publications

Li, Kato, Chuang: "Pivotal role of the C-terminal DW-motif in mediating inhibition of pyruvate dehydrogenase kinase 2 by dichloroacetate." in: The Journal of biological chemistry, Vol. 284, Issue 49, pp. 34458-67, 2009

Fencl, Janda, Bláhová, Hríbal, Stekrová, Puchmajerová, Seeman: "Genotype-phenotype correlation in children with autosomal dominant polycystic kidney disease." in: Pediatric nephrology (Berlin, Germany), Vol. 24, Issue 5, pp. 983-9, 2009

Sun, Zhou, Chang, McFate, Verma, Califano: "Mitochondrial mutations contribute to HIF1alpha accumulation via increased reactive oxygen species and up-regulated pyruvate dehydrogenease kinase 2 in head and neck squamous cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 2, pp. 476-84, 2009

Baker, Hetherington, Reeves, Müller, Aitken: "The rat sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification." in: Proteomics, Vol. 8, Issue 11, pp. 2312-21, 2008

Images

Product Details anti-PDK2 Antibody Target Details PDK2 Application Details Handling References for anti-PDK2 Antibody (ABIN954072) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-PDK2 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 2) (ABIN954072) Flow cytometric analysis of Jurkat cells using PDK2 Antibody  (bottom histogram) comp...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PDK2 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 2) (ABIN954072) Immunohistochemistry analysis in formalin fixed and paraffin embedded skeletal muscle...
Western Blotting (WB) image for anti-PDK2 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 2) (ABIN954072) Western blot analysis of PDK2 Antibody  in Jurkat cell line lysates (35ug/lane). PDK2...