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anti-Human PDK2 antibody for Immunocytochemistry

Recommended PDK2 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) Antibodies
  • 3j828
  • MGC52849
  • MGC132353
  • PDHK2
  • Pdk
  • PDK2
  • PDKII
  • zgc:56209
Epitope
AA 379-392, C-Term
20
17
16
15
8
5
5
3
3
2
2
2
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
122
61
32
5
3
1
Host
Rabbit
104
38
Clonality
Polyclonal
Conjugate
This PDK2 antibody is un-conjugated
9
9
8
7
7
7
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
121
84
62
27
17
11
4
3
1
1
1
1
Supplier
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Catalog No. ABIN3044044
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN2626695 ICC IHC (p) WB Rabbit IgG AA 379-392 Log in to see Polyclonal
0.0009962841 ABIN4344598 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0009962841 ABIN3032416 ICC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

Similar anti-PDK2 Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 10 Antibodies 73 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 3 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 7 Antibodies 61 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies 26 Antibodies
Western Blotting (WB) 21 Antibodies
Flow Cytometry (FACS) 17 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunofluorescence (IF) 1 Antibodies
RNA Interference (RNAi) 1 Antibodies

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) Antibodies
Epitope AA 379-392, C-Term
(20), (17), (16), (15), (8), (5), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(122), (61), (32), (5), (3), (1)
Host Rabbit
(104), (38)
Clonality
Polyclonal
Conjugate This PDK2 antibody is un-conjugated
(9), (9), (8), (7), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(121), (84), (62), (27), (17), (11), (4), (3), (1), (1), (1), (1)
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Product Details anti-PDK2 Antibody

Target Details PDK2 Application Details Handling Images
Sequence WRHYQTIQEA GDWC
Cross-Reactivity (Details) Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.
Characteristics Rabbit IgG polyclonal antibody for[Pyruvate dehydrogenase[lipoamide]] kinase isozyme 2, mitochondrial(PDK2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: pyruvate dehydrogenase kinase, isozyme 2
Protein Name: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2, mitochondrial
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human PDK2(379-392aa WRHYQTIQEAGDWC), identical to the related rat and mouse sequences.
Isotype IgG

Target Details PDK2

Product Details anti-PDK2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PDK2 (PDK2 Antibody Abstract)
Background PDK2(Pyruvate Dehydrogenase Kinase Isoenzyme 2), is an enzyme that in humans is encoded by the PDK2 gene. To find human PDKs, Gudi et al.(1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. The human PDK2 gene encodes a protein with a predicted molecular mass of 46,181 Da that shares 96 % identity with that of the rat PDK2 gene. Northern blot analysis showed that the PDK2 message was found in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.
UniProt Q15119
Research Area Cancer, Metabolism
Pathways PI3K-Akt Signaling, RTK Signaling

Application Details

Product Details anti-PDK2 Antibody Target Details PDK2 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Predicted Species: Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-PDK2 Antibody Target Details PDK2 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-PDK2 Antibody Target Details PDK2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-PDK2 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 2) (AA 379-392) (ABIN3044044) Anti-PDK2 antibody, IHC(P) IHC(P): Human Mammary Cancer Tissue
Immunohistochemistry (IHC) image for anti-PDK2 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 2) (AA 379-392) (ABIN3044044) Anti-PDK2 antibody, ICC ICC: HELA Cell
Western Blotting (WB) image for anti-PDK2 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 2) (AA 379-392) (ABIN3044044) Anti-PDK2 antibody, Western blotting Lane 1: Rat Heart Tissue Lysate Lane 2: HELA Cel...
Immunohistochemistry (IHC) image for anti-PDK2 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 2) (AA 379-392) (ABIN3044044) Anti-PDK2 antibody, IHC(P) IHC(P): Rat Brain Tissue