anti-Human PDK2 antibody for Western Blotting

Recommended PDK2 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) Antibodies
  • 3j828
  • MGC52849
  • MGC132353
  • Pdk
  • zgc:56209
  • PDK2
  • PDHK2
  • PDKII
  • pyruvate dehydrogenase kinase, isozyme 4
  • pyruvate dehydrogenase kinase, isoenzyme 2
  • pyruvate dehydrogenase kinase, isozyme 2
  • pdk4
  • pdk2
  • PDK2
  • Pdk2
Epitope
AA 378-407, C-Term
17
12
11
10
10
8
5
4
3
3
2
2
2
2
1
1
1
1
1
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
126
78
43
11
5
2
2
2
1
1
1
1
1
1
Host
Rabbit
103
40
Clonality
Polyclonal
Conjugate
This PDK2 antibody is un-conjugated
9
9
8
7
7
7
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
124
86
65
29
20
13
5
2
1
1
1
1
1
Options
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Catalog No. ABIN391037
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1592565 ABIN1535242 ELISA WB Rabbit IgG Log in to see Polyclonal 1
3.1592565 ABIN653784 FACS IHC (p) WB Rabbit Ig Log in to see Polyclonal 2
3.1592565 ABIN3044044 ICC IHC (p) WB Rabbit IgG AA 379-392, C-Term Log in to see Polyclonal
3.1592565 ABIN4344598 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
3.1592565 ABIN657996 IHC (p) WB Rabbit Ig AA 78-107, N-Term Log in to see Polyclonal
3.1592565 ABIN3186377 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
3.1592565 ABIN562160 IHC (p) RNAi ELISA WB Mouse IgG2a kappa AA 187-276, partial Log in to see 2G1 4
3.1592565 ABIN781865 EIA IHC (p) WB Mouse IgG2a Log in to see 2G1
3.1592565 ABIN781866 EIA IHC (p) WB Mouse IgG2a Log in to see 5F8
3.1592565 ABIN659059 WB Mouse IgG1, kappa Log in to see 180CT10-2-3
3.1592565 ABIN2457915 ELISA WB Rabbit Log in to see Polyclonal
3.1592565 ABIN4890304 IHC IHC (p) WB Rabbit Log in to see Polyclonal
3.1592565 ABIN3028959 ELISA WB Mouse IgG1, kappa Log in to see 180CT10-2-3
3.1592565 ABIN518756 IHC (p) ELISA WB Mouse IgG2a kappa AA 187-276, partial Log in to see 5F8
3.1592565 ABIN518757 ELISA WB Mouse IgG2b kappa AA 1-407, full length Log in to see 2D12
3.1592565 ABIN518755 WB Mouse AA 1-407, full length Log in to see Polyclonal
3.1592565 ABIN562159 ELISA WB Mouse AA 187-276, partial Log in to see Polyclonal
3.1592565 ABIN4912898 IHC (p) WB Mouse IgG1 kappa Log in to see 180CT10-2-3
3.1592565 ABIN3032416 ICC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal
3.1592565 ABIN2426341 IHC ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-PDK2 antibody for Western Blotting

Similar anti-PDK2 Antibodies

Application / Reactivity Mouse (Murine) Human Rat (Rattus) Monkey
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Western Blotting (WB) 109 Antibodies 61 Antibodies 30 Antibodies 11 Antibodies
RNA Interference (RNAi) 1 Antibodies
Immunoprecipitation (IP) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 30 Antibodies 12 Antibodies 8 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 62 Antibodies 26 Antibodies 15 Antibodies 8 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies 13 Antibodies

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) Antibodies
Epitope AA 378-407, C-Term
(17), (12), (11), (10), (10), (8), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(126), (78), (43), (11), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(103), (40)
Clonality (Clone)
Polyclonal   ( )
Conjugate This PDK2 antibody is un-conjugated
(9), (9), (8), (7), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(124), (86), (65), (29), (20), (13), (5), (2), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-PDK2 Antibody

Target Details PDK2 Application Details Handling ProductDetails: References for anti-PDK2 antibody (ABIN391037) Images
Specificity This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-term region of human PDK2.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Immunogen This PDK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-407 AA from the C-terminal region of human PDK2.
Clone RB03037
Isotype Ig

Target Details PDK2

Product Details anti-PDK2 Antibody Application Details Handling ProductDetails: References for anti-PDK2 antibody (ABIN391037) Images back to top
Antigen
Alternative Name PDK2 (PDK2 Antibody Abstract)
Background Synonyms: Pyruvate dehydrogenase kinase isoform 2
Molecular Weight 46153 DA
Gene ID 5164
UniProt Q15119
Research Area Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure
Pathways PI3K-Akt Signaling, RTK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-PDK2 Antibody Target Details PDK2 Handling ProductDetails: References for anti-PDK2 antibody (ABIN391037) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100
Restrictions For Research Use only

Handling

Product Details anti-PDK2 Antibody Target Details PDK2 Application Details ProductDetails: References for anti-PDK2 antibody (ABIN391037) Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

ProductDetails: References for anti-PDK2 antibody (ABIN391037)

Product Details anti-PDK2 Antibody Target Details PDK2 Application Details Handling Images back to top
Product cited in:

Hurd, Collins, Abakumova, Chouchani, Baranowski, Fearnley, Prime, Murphy, James: "Inactivation of pyruvate dehydrogenase kinase 2 by mitochondrial reactive oxygen species." in: The Journal of biological chemistry, Vol. 287, Issue 42, pp. 35153-60, 2012

Love, LeBlanc, Inglis, Bradley, Choptiany, Heigenhauser, Peters: "The relationship between human skeletal muscle pyruvate dehydrogenase phosphatase activity and muscle aerobic capacity." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 111, Issue 2, pp. 427-34, 2011

Dunford, Herbst, Jeoung, Gittings, Inglis, Vandenboom, LeBlanc, Harris, Peters: "PDH activation during in vitro muscle contractions in PDH kinase 2 knockout mice: effect of PDH kinase 1 compensation." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 300, Issue 6, pp. R1487-93, 2011

Nicholls, Chu, Abbruzzese, Tremblay, Hardy: "Mechanism of a genetically encoded dark-to-bright reporter for caspase activity." in: The Journal of biological chemistry, Vol. 286, Issue 28, pp. 24977-86, 2011

McClenaghan, Scullion, Mion, Hewage, Malthouse, Flatt, Newsholme, Brennan: "Prolonged L-alanine exposure induces changes in metabolism, Ca(2+) handling and desensitization of insulin secretion in clonal pancreatic beta-cells." in: Clinical science (London, England : 1979), Vol. 116, Issue 4, pp. 341-51, 2009

Sun, Zhou, Chang, McFate, Verma, Califano: "Mitochondrial mutations contribute to HIF1alpha accumulation via increased reactive oxygen species and up-regulated pyruvate dehydrogenease kinase 2 in head and neck squamous cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 2, pp. 476-84, 2009

Richards, Sardella, Schulte: "Regulation of pyruvate dehydrogenase in the common killifish, Fundulus heteroclitus, during hypoxia exposure." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 295, Issue 3, pp. R979-90, 2008

Zhang, Ma, Sadana, Chowdhury, Gaillard, Wang, McDonnell, Unterman, Elam, Park: "Estrogen-related receptors stimulate pyruvate dehydrogenase kinase isoform 4 gene expression." in: The Journal of biological chemistry, Vol. 281, Issue 52, pp. 39897-906, 2006

Churchill, Murriel, Chen, Mochly-Rosen, Szweda: "Reperfusion-induced translocation of deltaPKC to cardiac mitochondria prevents pyruvate dehydrogenase reactivation." in: Circulation research, Vol. 97, Issue 1, pp. 78-85, 2005

Images

Product Details anti-PDK2 Antibody Target Details PDK2 Application Details Handling ProductDetails: References for anti-PDK2 antibody (ABIN391037) back to top
Supplier Images
Western Blotting (WB) image for anti-PDK2 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 2) (AA 378-407) (ABIN391037) Western blot analysis of PDK2 Antibody (C-term) (ABIN391037) in mouse intestine tissu...
Western Blotting (WB) image for anti-PDK2 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 2) (AA 378-407) (ABIN391037) Western blot analysis of PDK2 (arrow) using PDK2 Antibody (C-term) (ABIN391037). 293 ...
Immunohistochemistry (IHC) image for anti-PDK2 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 2) (AA 378-407) (ABIN391037) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...