|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Monkey|
|Western Blotting (WB)||106 Antibodies||43 Antibodies||22 Antibodies||4 Antibodies|
|RNA Interference (RNAi)||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||24 Antibodies||9 Antibodies||7 Antibodies||1 Antibodies|
|Immunohistochemistry (IHC)||60 Antibodies||10 Antibodies||8 Antibodies||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies||11 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Antigen||Pyruvate Dehydrogenase Kinase, Isozyme 2 (PDK2) Antibodies|
|Epitope||AA 378-407, C-Term Alternatives|
|Reactivity||Human, Monkey, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This PDK2 antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|9 references available|
|Supplier||Log in to see|
Product Details anti-PDK2 AntibodyTarget Details PDK2 Application Details Handling References for anti-PDK2 Antibody (ABIN391037) Images
|Specificity||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-term region of human PDK2.|
|Purification||This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Immunogen||This PDK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 378-407 AA from the C-terminal region of human PDK2.|
Target Details PDK2Product Details anti-PDK2 Antibody Application Details Handling References for anti-PDK2 Antibody (ABIN391037) Images back to top
|Alternative Name||PDK2 (PDK2 Antibody Abstract)|
|Background||Synonyms: Pyruvate dehydrogenase kinase isoform 2|
|Molecular Weight||46153 DA|
|Research Area||Phospho-specific antibodies, Cell Signaling, Protein Modifications, Metabolism, Cell Structure|
|Pathways||PI3K-Akt Signaling, RTK Signaling|
Application DetailsProduct Details anti-PDK2 Antibody Target Details PDK2 Handling References for anti-PDK2 Antibody (ABIN391037) Images back to top
|Application Notes||WB = 1:1000, IHC (p) = 1:50-100|
|Restrictions||For Research Use only|
HandlingProduct Details anti-PDK2 Antibody Target Details PDK2 Application Details References for anti-PDK2 Antibody (ABIN391037) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
|Storage Comment||Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.|
|Expiry Date||6 months|
References for anti-PDK2 Antibody (ABIN391037)Product Details anti-PDK2 Antibody Target Details PDK2 Application Details Handling Images back to top
|Product cited in:||
Hurd, Collins, Abakumova et al.: "Inactivation of pyruvate dehydrogenase kinase 2 by mitochondrial reactive oxygen species." in: The Journal of biological chemistry, Vol. 287, Issue 42, pp. 35153-60, 2012 (PubMed).
Love, LeBlanc, Inglis et al.: "The relationship between human skeletal muscle pyruvate dehydrogenase phosphatase activity and muscle aerobic capacity." in: Journal of applied physiology (Bethesda, Md. : 1985), Vol. 111, Issue 2, pp. 427-34, 2011 (PubMed).
Nicholls, Chu, Abbruzzese et al.: "Mechanism of a genetically encoded dark-to-bright reporter for caspase activity." in: The Journal of biological chemistry, Vol. 286, Issue 28, pp. 24977-86, 2011 (PubMed).
Dunford, Herbst, Jeoung et al.: "PDH activation during in vitro muscle contractions in PDH kinase 2 knockout mice: effect of PDH kinase 1 compensation." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 300, Issue 6, pp. R1487-93, 2011 (PubMed).
Sun, Zhou, Chang et al.: "Mitochondrial mutations contribute to HIF1alpha accumulation via increased reactive oxygen species and up-regulated pyruvate dehydrogenease kinase 2 in head and neck squamous cell carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 15, Issue 2, pp. 476-84, 2009 (PubMed).
McClenaghan, Scullion, Mion et al.: "Prolonged L-alanine exposure induces changes in metabolism, Ca(2+) handling and desensitization of insulin secretion in clonal pancreatic beta-cells." in: Clinical science (London, England : 1979), Vol. 116, Issue 4, pp. 341-51, 2009 (PubMed).
Richards, Sardella, Schulte: "Regulation of pyruvate dehydrogenase in the common killifish, Fundulus heteroclitus, during hypoxia exposure." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 295, Issue 3, pp. R979-90, 2008 (PubMed).
Zhang, Ma, Sadana et al.: "Estrogen-related receptors stimulate pyruvate dehydrogenase kinase isoform 4 gene expression." in: The Journal of biological chemistry, Vol. 281, Issue 52, pp. 39897-906, 2006 (PubMed).
Churchill, Murriel, Chen et al.: "Reperfusion-induced translocation of deltaPKC to cardiac mitochondria prevents pyruvate dehydrogenase reactivation." in: Circulation research, Vol. 97, Issue 1, pp. 78-85, 2005 (PubMed).
ImagesProduct Details anti-PDK2 Antibody Target Details PDK2 Application Details Handling References for anti-PDK2 Antibody (ABIN391037) back to top