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anti-Cow (Bovine) PDK3 antibody for Western Blotting

Recommended PDK3 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) Antibodies
  • pdk3
  • PDK3
  • 2610001M10Rik
  • AI035637
  • pyruvate dehydrogenase kinase, isozyme 3a
  • pyruvate dehydrogenase kinase, isozyme 3
  • pyruvate dehydrogenase kinase, isoenzyme 3
  • pdk3a
  • PDK3
  • pdk3
  • LOC100226102
  • Pdk3
Epitope
N-Term
16
11
11
9
8
5
3
2
2
2
2
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Zebrafish
63
31
7
3
3
2
2
2
2
2
2
2
1
1
Host
Rabbit
74
9
Clonality
Polyclonal
Conjugate
This PDK3 antibody is un-conjugated
6
6
6
6
6
6
Application
Immunohistochemistry (IHC), Western Blotting (WB)
77
52
22
5
4
3
3
3
1
1
1
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Catalog No. ABIN2782501
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0922565 ABIN2782500 WB Rabbit Middle Region Log in to see Polyclonal 1
1 ABIN321054 WB Rabbit IgG AA 74-123 Log in to see Polyclonal
1 ABIN469171 WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-PDK3 antibody for Western Blotting

Similar anti-PDK3 Antibodies

Application / Reactivity Mouse (Murine) Human Cow (Bovine) Dog (Canine) Rat (Rattus) Pig (Porcine) Guinea Pig Horse (Equine) Rabbit Zebrafish
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Western Blotting (WB) 59 Antibodies 30 Antibodies 4 Antibodies 3 Antibodies 7 Antibodies 3 Antibodies 4 Antibodies 3 Antibodies 3 Antibodies 3 Antibodies
Immunoprecipitation (IP) 2 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies 3 Antibodies
Immunohistochemistry (IHC) 6 Antibodies 19 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) Antibodies
Epitope N-Term
(16), (11), (11), (9), (8), (5), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Zebrafish
(63), (31), (7), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(74), (9)
Clonality
Polyclonal
Conjugate This PDK3 antibody is un-conjugated
(6), (6), (6), (6), (6), (6)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(77), (52), (22), (5), (4), (3), (3), (3), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PDK3 Antibody

Target Details PDK3 Application Details Handling References for anti-PDK3 Antibody (ABIN2782501) Images
Blocking Peptide Blocking peptide for this product available: ABIN981541
Sequence RPSVGLVQSW YMQSFLELLE YENKSPEDPQ VLDNFLQVLI KVRNRHNDVV
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 85%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 75%
Characteristics This is a rabbit polyclonal antibody against PDK3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PDK3

Target Details PDK3

Product Details anti-PDK3 Antibody Application Details Handling References for anti-PDK3 Antibody (ABIN2782501) Images back to top
Antigen
Alternative Name PDK3 (PDK3 Antibody Abstract)
Background PDK3 belongs to the PDK/BCKDK protein kinase family. It contains 1 histidine kinase domain. PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Alias Symbols: -
Protein Interaction Partner: APP, UBD, UBC, ICT1, EGFR, DLAT, PDHA1,
Protein Size: 406
Molecular Weight 47 kDa
Gene ID 5165
NCBI Accession NM_005391, NP_005382
UniProt Q15120
Research Area Signaling, Metabolism
Pathways PI3K-Akt Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-PDK3 Antibody Target Details PDK3 Handling References for anti-PDK3 Antibody (ABIN2782501) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 406 AA

Restrictions For Research Use only

Handling

Product Details anti-PDK3 Antibody Target Details PDK3 Application Details References for anti-PDK3 Antibody (ABIN2782501) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PDK3 Antibody (ABIN2782501)

Product Details anti-PDK3 Antibody Target Details PDK3 Application Details Handling Images back to top
Background publications

Degenhardt, Saramäki, Malinen, Rieck, Väisänen, Huotari, Herzig, Müller, Carlberg: "Three members of the human pyruvate dehydrogenase kinase gene family are direct targets of the peroxisome proliferator-activated receptor beta/delta." in: Journal of molecular biology, Vol. 372, Issue 2, pp. 341-55, 2007

Images

Product Details anti-PDK3 Antibody Target Details PDK3 Application Details Handling References for anti-PDK3 Antibody (ABIN2782501) back to top
Supplier Images
Western Blotting (WB) image for anti-PDK3 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 3) (N-Term) (ABIN2782501) anti-Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) (N-Term) antibody