anti-Human PDK3 antibody for Western Blotting

Recommended PDK3 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) Antibodies
  • pdk3
  • PDK3
  • 2610001M10Rik
  • AI035637
  • pyruvate dehydrogenase kinase, isozyme 3a
  • pyruvate dehydrogenase kinase, isozyme 3
  • pyruvate dehydrogenase kinase, isoenzyme 3
  • pdk3a
  • PDK3
  • pdk3
  • LOC100226102
  • Pdk3
Epitope
Middle Region
16
12
12
10
8
5
3
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
65
33
8
3
3
3
2
2
2
2
2
2
1
1
Host
Rabbit
77
9
Clonality
Polyclonal
Conjugate
This PDK3 antibody is un-conjugated
6
6
6
6
6
6
Application
Western Blotting (WB)
80
52
24
6
4
4
3
3
1
1
1
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Catalog No. ABIN2782500
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9634955 ABIN2782501 IHC WB Rabbit N-Term Log in to see Polyclonal 1
2.9634955 ABIN442546 ICC IF IHC (p) IHC WB Rabbit IgG Center Log in to see Polyclonal 2
2.9634955 ABIN391038 WB Rabbit Ig AA 1-30, N-Term Log in to see Polyclonal 2
2.9634955 ABIN562161 ELISA WB Mouse IgG2a kappa AA 174-263, partial Log in to see 2B11 3
2.9634955 ABIN2737343 WB Rabbit IgG Log in to see Polyclonal
2.9634955 ABIN652286 FACS WB Rabbit Ig Log in to see Polyclonal
2.9634955 ABIN1539888 WB Mouse IgG2b Log in to see 431CT6-1
2.9634955 ABIN2458989 ELISA WB Rabbit Log in to see Polyclonal
2.9634955 ABIN4344599 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
2.9634955 ABIN4890306 WB Rabbit Log in to see Polyclonal
2.9634955 ABIN5078730 ICC IF WB Rabbit IgG Log in to see Polyclonal
2.9634955 ABIN631033 WB Rabbit Middle Region Log in to see Polyclonal
2.9634955 ABIN631065 WB Rabbit N-Term Log in to see Polyclonal
2.9634955 ABIN518760 ELISA WB Mouse IgG1 AA 174-263, partial Log in to see 1G11
2.9634955 ABIN518759 ELISA WB Mouse IgG1 kappa AA 174-263, partial Log in to see 3A1
1 ABIN5610936 EIA WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
1 ABIN321054 WB Rabbit IgG AA 74-123 Log in to see Polyclonal
1 ABIN469171 WB Rabbit C-Term Log in to see Polyclonal
1 ABIN1500119 WB Mouse IgG2b Log in to see
1 ABIN5661498 IHC WB Rabbit Log in to see Polyclonal

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) Antibodies
Epitope Middle Region
(16), (12), (12), (10), (8), (5), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(65), (33), (8), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(77), (9)
Clonality
Polyclonal
Conjugate This PDK3 antibody is un-conjugated
(6), (6), (6), (6), (6), (6)
Application Western Blotting (WB)
(80), (52), (24), (6), (4), (4), (3), (3), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PDK3 Antibody

Target Details PDK3 Application Details Handling References for anti-PDK3 antibody (ABIN2782500) Images
Blocking Peptide Blocking peptide for this product available: ABIN981540
Sequence IYLKALSSES FERLPVFNKS AWRHYKTTPE ADDWSNPSSE PRDASKYKAK
Predicted Reactivity Cow: 100%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 85%
Characteristics This is a rabbit polyclonal antibody against PDK3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human PDK3

Target Details PDK3

Product Details anti-PDK3 Antibody Application Details Handling References for anti-PDK3 antibody (ABIN2782500) Images back to top
Antigen
Alternative Name PDK3 (PDK3 Antibody Abstract)
Background PDK3 belongs to the PDK/BCKDK protein kinase family. It contains 1 histidine kinase domain. PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Alias Symbols: -
Protein Interaction Partner: APP, UBD, UBC, ICT1, EGFR, DLAT, PDHA1,
Protein Size: 406
Molecular Weight 47 kDa
Gene ID 5165
NCBI Accession NM_005391, NP_005382
UniProt Q15120
Research Area Signaling, Metabolism
Pathways PI3K-Akt Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-PDK3 Antibody Target Details PDK3 Handling References for anti-PDK3 antibody (ABIN2782500) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 406 AA

Restrictions For Research Use only

Handling

Product Details anti-PDK3 Antibody Target Details PDK3 Application Details References for anti-PDK3 antibody (ABIN2782500) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PDK3 antibody (ABIN2782500)

Product Details anti-PDK3 Antibody Target Details PDK3 Application Details Handling Images back to top
Background publications

Degenhardt, Saramäki, Malinen, Rieck, Väisänen, Huotari, Herzig, Müller, Carlberg: "Three members of the human pyruvate dehydrogenase kinase gene family are direct targets of the peroxisome proliferator-activated receptor beta/delta." in: Journal of molecular biology, Vol. 372, Issue 2, pp. 341-55, 2007

Images

Product Details anti-PDK3 Antibody Target Details PDK3 Application Details Handling References for anti-PDK3 antibody (ABIN2782500) back to top
Supplier Images
Western Blotting (WB) image for anti-Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) (Middle Region) antibody (ABIN2782500) WB Suggested Anti-PDK3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...