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anti-Mouse (Murine) PDK3 antibody for Enzyme Immunoassay

Recommended PDK3 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) Antibodies
  • 2610001M10Rik
  • AI035637
  • PDK3
  • pdk3
Epitope
N-Term
20
17
15
15
4
3
2
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine)
63
27
6
2
2
2
1
Host
Rabbit
73
10
Clonality
Polyclonal
Conjugate
This PDK3 antibody is un-conjugated
6
6
6
6
6
6
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
74
52
18
4
3
2
1
1
1
Supplier
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Catalog No. ABIN359097
$ 401.50
Plus shipping costs $45.00

Top referenced anti-PDK3 antibody for Enzyme Immunoassay

  • Human Polyclonal PDK3 Primary Antibody for EIA, WB - ABIN359097 (1 Publications): McFate, Mohyeldin, Lu, Thakar, Henriques, Halim, Wu, Schell, Tsang, Teahan, Zhou, Califano, Jeoung, Harris, Verma: Pyruvate dehydrogenase complex activity controls metabolic and malignant phenotype in cancer cells. in The Journal of biological chemistry 2008 (PubMed)

Similar anti-PDK3 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 14 Antibodies 38 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunohistochemistry (IHC) 15 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies 2 Antibodies
Western Blotting (WB) 24 Antibodies 59 Antibodies
ELISA (Capture) 1 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunoprecipitation (IP) 2 Antibodies

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) Antibodies
Epitope N-Term
(20), (17), (15), (15), (4), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(63), (27), (6), (2), (2), (2), (1)
Host Rabbit
(73), (10)
Clonality
Polyclonal
Conjugate This PDK3 antibody is un-conjugated
(6), (6), (6), (6), (6), (6)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(74), (52), (18), (4), (3), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PDK3 Antibody

Target Details PDK3 Application Details Handling References for anti-PDK3 Antibody (ABIN359097) Images
Specificity This antibody reacts to PDK3.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PDK3.
Isotype Ig

Target Details PDK3

Product Details anti-PDK3 Antibody Application Details Handling References for anti-PDK3 Antibody (ABIN359097) Images back to top
Antigen
Alternative Name PDK3 (PDK3 Antibody Abstract)
Background PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.Synonyms: Pyruvate dehydrogenase kinase isoform 3, Pyruvate dehydrogenase lipoamide kinase isozyme 3 mitochondrial
Gene ID 5165, 9606
UniProt Q15120
Research Area Signaling, Metabolism
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-PDK3 Antibody Target Details PDK3 Handling References for anti-PDK3 Antibody (ABIN359097) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PDK3 Antibody Target Details PDK3 Application Details References for anti-PDK3 Antibody (ABIN359097) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PDK3 Antibody (ABIN359097)

Product Details anti-PDK3 Antibody Target Details PDK3 Application Details Handling Images back to top
Background publications

McFate, Mohyeldin, Lu, Thakar, Henriques, Halim, Wu, Schell, Tsang, Teahan, Zhou, Califano, Jeoung, Harris, Verma: "Pyruvate dehydrogenase complex activity controls metabolic and malignant phenotype in cancer cells." in: The Journal of biological chemistry, Vol. 283, Issue 33, pp. 22700-8, 2008

Images

Product Details anti-PDK3 Antibody Target Details PDK3 Application Details Handling References for anti-PDK3 Antibody (ABIN359097) back to top
Supplier Images
Western Blotting (WB) image for anti-PDK3 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 3) (N-Term) (ABIN359097) Western blot analysis of PDK3 (arrow) using rabbit polyclonal PDK3 Antibody