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anti-Mouse (Murine) PDK3 antibody for Western Blotting

Recommended PDK3 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) Antibodies
  • 2610001M10Rik
  • AI035637
  • PDK3
  • pdk3
Epitope
Middle Region
21
17
15
15
4
3
2
2
2
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
62
27
5
1
1
1
Host
Rabbit
72
10
Clonality
Polyclonal
Conjugate
This PDK3 antibody is un-conjugated
6
6
6
6
6
6
Application
Western Blotting (WB)
73
52
18
4
3
2
1
1
1
1
Supplier
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Catalog No. ABIN2782500
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.123037994 ABIN2782501 WB Rabbit N-Term Log in to see Polyclonal 1
0.123037994 ABIN359097 EIA WB Rabbit Ig N-Term Log in to see Polyclonal 1
0.123037994 ABIN658195 IHC (p) WB Rabbit Ig AA 387-414, C-Term Log in to see Polyclonal 1
0.123037994 ABIN631033 WB Rabbit Middle Region Log in to see Polyclonal
0.123037994 ABIN2496018 IHC WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.0011806273 ABIN1922657 IHC ELISA WB APC Rabbit IgG C-Term, AA 386-414 Log in to see Polyclonal
0.0011806273 ABIN1922656 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term, AA 386-414 Log in to see Polyclonal
0.0011806273 ABIN1922660 IHC ELISA WB PE Rabbit IgG C-Term, AA 386-414 Log in to see Polyclonal
0.0011806273 ABIN1922658 IHC ELISA WB Biotin Rabbit IgG C-Term, AA 386-414 Log in to see Polyclonal
0.0011806273 ABIN1922659 IHC ELISA WB FITC Rabbit IgG C-Term, AA 386-414 Log in to see Polyclonal
0.0011806273 ABIN1922661 IHC ELISA WB HRP Rabbit IgG C-Term, AA 386-414 Log in to see Polyclonal
0.0011806273 ABIN2737343 WB Rabbit IgG Log in to see Polyclonal
0.0011806273 ABIN1811635 IHC (p) WB Rabbit AA 386-414 Log in to see Polyclonal
0.0011806273 ABIN2332641 IHC ELISA WB Rabbit IgG C-Term, AA 386-414 Log in to see Polyclonal
0.0011806273 ABIN2332644 IHC ELISA WB APC Rabbit IgG C-Term, AA 386-414 Log in to see Polyclonal
0.0011806273 ABIN2332647 IHC ELISA WB HRP Rabbit IgG C-Term, AA 386-414 Log in to see Polyclonal
0.0011806273 ABIN2332646 IHC ELISA WB FITC Rabbit IgG C-Term, AA 386-414 Log in to see Polyclonal
0.0011806273 ABIN2332648 IHC ELISA WB PE Rabbit IgG C-Term, AA 386-414 Log in to see Polyclonal
0.0011806273 ABIN2332645 IHC ELISA WB Biotin Rabbit IgG C-Term, AA 386-414 Log in to see Polyclonal
0.0011806273 ABIN2332643 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term, AA 386-414 Log in to see Polyclonal

Top referenced anti-PDK3 antibody for Western Blotting

Similar anti-PDK3 Antibodies

Application / Reactivity Human Mouse (Murine) Chicken Cow (Bovine) Dog (Canine) Rat (Rattus) Pig (Porcine)
Western Blotting (WB) 58 Antibodies 24 Antibodies 2 Antibodies 2 Antibodies 2 Antibodies 5 Antibodies 1 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 4 Antibodies 15 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Flow Cytometry (FACS) 4 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 3 (PDK3) Antibodies
Epitope Middle Region
(21), (17), (15), (15), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(62), (27), (5), (1), (1), (1)
Host Rabbit
(72), (10)
Clonality
Polyclonal
Conjugate This PDK3 antibody is un-conjugated
(6), (6), (6), (6), (6), (6)
Application Western Blotting (WB)
(73), (52), (18), (4), (3), (2), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PDK3 Antibody

Target Details PDK3 Application Details Handling References for anti-PDK3 Antibody (ABIN2782500) Images
Blocking Peptide Blocking peptide for this product available: ABIN981540
Sequence IYLKALSSES FERLPVFNKS AWRHYKTTPE ADDWSNPSSE PRDASKYKAK
Predicted Reactivity Bovine : 100 %, Chicken : 100 %, Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Pig : 100 %, Rat : 100 %, Dog : 90 %, Horse : 90 %, Zebrafish : 80 %
Characteristics This is a rabbit polyclonal antibody against PDK3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human PDK3

Target Details PDK3

Product Details anti-PDK3 Antibody Application Details Handling References for anti-PDK3 Antibody (ABIN2782500) Images back to top
Antigen
Alternative Name PDK3 (PDK3 Antibody Abstract)
Background PDK3 belongs to the PDK/BCKDK protein kinase family. It contains 1 histidine kinase domain. PDK3 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
Molecular Weight 47 kDa
Gene ID 5165
NCBI Accession NM_005391, NP_005382
UniProt Q15120
Research Area Signaling, Metabolism
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-PDK3 Antibody Target Details PDK3 Handling References for anti-PDK3 Antibody (ABIN2782500) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 406 AA

Restrictions For Research Use only

Handling

Product Details anti-PDK3 Antibody Target Details PDK3 Application Details References for anti-PDK3 Antibody (ABIN2782500) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PDK3 Antibody (ABIN2782500)

Product Details anti-PDK3 Antibody Target Details PDK3 Application Details Handling Images back to top
Background publications

Degenhardt, Saramäki, Malinen, Rieck, Väisänen, Huotari, Herzig, Müller, Carlberg: "Three members of the human pyruvate dehydrogenase kinase gene family are direct targets of the peroxisome proliferator-activated receptor beta/delta." in: Journal of molecular biology, Vol. 372, Issue 2, pp. 341-55, 2007

Images

Product Details anti-PDK3 Antibody Target Details PDK3 Application Details Handling References for anti-PDK3 Antibody (ABIN2782500) back to top
Supplier Images
Western Blotting (WB) image for anti-PDK3 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 3) (Middle Region) (ABIN2782500) WB Suggested Anti-PDK3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...