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anti-Cow (Bovine) PDK4 antibody for Immunohistochemistry

Recommended PDK4 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
  • AV005916
Epitope
N-Term
22
22
13
12
10
6
5
5
5
5
5
4
3
3
3
3
3
3
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
116
47
11
Host
Rabbit
119
19
Clonality
Polyclonal
Conjugate
This PDK4 antibody is un-conjugated
9
8
7
7
7
6
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
118
78
54
14
10
4
2
2
1
Supplier
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Catalog No. ABIN2777592
$ 289.00
Plus shipping costs $45.00

Top referenced anti-PDK4 antibody for Immunohistochemistry

Similar anti-PDK4 Antibodies

Application / Reactivity Human Mouse (Murine) Cow (Bovine) Dog (Canine) Pig (Porcine) Rat (Rattus)
Western Blotting (WB) 103 Antibodies 41 Antibodies 5 Antibodies 1 Antibodies 1 Antibodies 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 13 Antibodies 4 Antibodies
Immunohistochemistry (IHC) 1 Antibodies 1 Antibodies 55 Antibodies 15 Antibodies 1 Antibodies 8 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
Epitope N-Term
(22), (22), (13), (12), (10), (6), (5), (5), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(116), (47), (11)
Host Rabbit
(119), (19)
Clonality
Polyclonal
Conjugate This PDK4 antibody is un-conjugated
(9), (8), (7), (7), (7), (6), (2), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(118), (78), (54), (14), (10), (4), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-PDK4 Antibody

Target Details PDK4 Application Details Handling References for anti-PDK4 Antibody (ABIN2777592) Images
Blocking Peptide Blocking peptide for this product available: ABIN981542
Sequence MKAARFVLRSAGSLNGAGLVPREVEHFSRYSPSPLSMKQL LDFGSENACE
Predicted Reactivity Horse : 100 %, Human : 100 %, Pig : 100 %, Rabbit : 100 %, Bovine : 90 %, Guinea pig : 90 %, Mouse : 90 %, Rat : 90 %, Dog : 90 %, Chicken : 80 %
Characteristics This is a rabbit polyclonal antibody against PDK4. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human PDK4

Target Details PDK4

Product Details anti-PDK4 Antibody Application Details Handling References for anti-PDK4 Antibody (ABIN2777592) Images back to top
Antigen
Alternative Name PDK4 (PDK4 Antibody Abstract)
Background PDK4 is a member of the PDK/BCKDK protein kinase family and is a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism.This gene is a member of the PDK/BCKDK protein kinase family and encodes a mitochondrial protein with a histidine kinase domain. This protein is located in the matrix of the mitrochondria and inhibits the pyruvate dehydrogenase complex by phosphorylating one of its subunits, thereby contributing to the regulation of glucose metabolism. Expression of this gene is regulated by glucocorticoids, retinoic acid and insulin.
Molecular Weight 46 kDa
Gene ID 5166
NCBI Accession NP_002603, NM_002612
UniProt Q16654
Research Area Signaling, Metabolism
Pathways PI3K-Akt Signaling, Hedgehog Signaling

Application Details

Product Details anti-PDK4 Antibody Target Details PDK4 Handling References for anti-PDK4 Antibody (ABIN2777592) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 411 AA

Restrictions For Research Use only

Handling

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details References for anti-PDK4 Antibody (ABIN2777592) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PDK4 Antibody (ABIN2777592)

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling Images back to top
Product cited in:

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, 2006

Background publications

Kimura, Wakamatsu, Suzuki, Ota, Nishikawa, Yamashita, Yamamoto, Sekine, Tsuritani, Wakaguri, Ishii, Sugiyama, Saito, Isono, Irie, Kushida, Yoneyama, Otsuka, Kanda, Yokoi, Kondo, Wagatsuma, Murakawa et al.: "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. ..." in: Genome research, Vol. 16, Issue 1, pp. 55-65, 2006

Images

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling References for anti-PDK4 Antibody (ABIN2777592) back to top
Supplier Images
Western Blotting (WB) image for anti-PDK4 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 4) (N-Term) (ABIN2777592) WB Suggested Anti-PDK4 Antibody Titration: 0.2-1 ug/ml
Positive Control:...
Western Blotting (WB) image for anti-PDK4 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 4) (N-Term) (ABIN2777592) Sample Type: Huh7 HepG2 (50ug)
Primary Antibody Dilution: 1:500
...
Immunohistochemistry (IHC) image for anti-PDK4 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 4) (N-Term) (ABIN2777592) anti-Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) (N-Term) antibody (Image 3)
Western Blotting (WB) image for anti-PDK4 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 4) (N-Term) (ABIN2777592) WB Suggested Anti-PDK4
Antibody Titration: 0.2-1 µg/mL
Positive Control...