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anti-Human PDK4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended PDK4 Antibody (supplied by: Log in to see )

Antigen
Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
  • AV005916
Epitope
Middle Region
26
24
23
17
13
13
12
10
5
5
5
4
3
3
3
3
3
2
1
1
1
1
1
1
1
Reactivity
Human
139
61
12
1
1
1
Host
Rabbit
142
19
Clonality
Polyclonal
Conjugate
This PDK4 antibody is un-conjugated
11
9
7
7
7
7
4
4
3
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
140
78
57
17
16
9
3
2
1
Supplier
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Catalog No. ABIN359100
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.34288716 ABIN359099 EIA IHC (p) WB Rabbit Ig Middle Region Log in to see Polyclonal 4
0.34288716 ABIN214801 IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.34288716 ABIN615174 EIA IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.34288716 ABIN1922668 IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0008982679 ABIN391039 IF IHC (p) WB Rabbit Ig AA 382-410, C-Term Log in to see Polyclonal 17
0.0008982679 ABIN391041 IF IHC (p) WB Rabbit Ig AA 83-111, Asp98, Center Log in to see Polyclonal
0.0008982679 ABIN391040 IHC (p) WB Rabbit Ig AA 250-277, Glu265 Log in to see Polyclonal
0.0008982679 ABIN2852398 IHC (p) ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0008982679 ABIN1860174 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 73-316 Log in to see Polyclonal
0.0008982679 ABIN1841119 IHC (p) WB Rabbit Glu265 Log in to see Polyclonal
0.0008982679 ABIN1841120 IF IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
0.0008982679 ABIN4344600 IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.0008982679 ABIN4344604 ICC IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0008982679 ABIN4344613 IHC IHC (p) Rabbit IgG Log in to see Polyclonal
0.0008982679 ABIN2767118 IHC (p) WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-PDK4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-PDK4 Antibodies

Application / Reactivity Human
ELISA 61 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 9 Antibodies
Immunofluorescence (IF) 18 Antibodies
Immunohistochemistry (IHC) 57 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Western Blotting (WB) 125 Antibodies

General

Antigen Pyruvate Dehydrogenase Kinase, Isozyme 4 (PDK4) Antibodies
Epitope Middle Region
(26), (24), (23), (17), (13), (13), (12), (10), (5), (5), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(139), (61), (12), (1), (1), (1)
Host Rabbit
(142), (19)
Clonality
Polyclonal
Conjugate This PDK4 antibody is un-conjugated
(11), (9), (7), (7), (7), (7), (4), (4), (3), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(140), (78), (57), (17), (16), (9), (3), (2), (1)
Pubmed 4 references available
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Product Details anti-PDK4 Antibody

Target Details PDK4 Application Details Handling References for anti-PDK4 Antibody (ABIN359100) Images
Specificity This antibody reacts to PDK4.
Purification Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center(D98) region of human PDK4.
Isotype Ig

Target Details PDK4

Product Details anti-PDK4 Antibody Application Details Handling References for anti-PDK4 Antibody (ABIN359100) Images back to top
Antigen
Alternative Name PDK4 (PDK4 Antibody Abstract)
Background PDK4 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.Synonyms: Pyruvate dehydrogenase kinase isoform 4, Pyruvate dehydrogenase lipoamide kinase isozyme 4 mitochondrial
Gene ID 5166, 9606
UniProt Q16654
Research Area Signaling, Metabolism
Pathways PI3K-Akt Signaling, Hedgehog Signaling

Application Details

Product Details anti-PDK4 Antibody Target Details PDK4 Handling References for anti-PDK4 Antibody (ABIN359100) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details References for anti-PDK4 Antibody (ABIN359100) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PDK4 Antibody (ABIN359100)

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling Images back to top
Background publications

Rosa, Di Rocco, Manco, Greco, Castagneto, Vidal, Mingrone: "Reduced PDK4 expression associates with increased insulin sensitivity in postobese patients." in: Obesity research, Vol. 11, Issue 2, pp. 176-82, 2003

Razeghi, Young, Ying, Depre, Uray, Kolesar, Shipley, Moravec, Davies, Frazier, Taegtmeyer: "Downregulation of metabolic gene expression in failing human heart before and after mechanical unloading." in: Cardiology, Vol. 97, Issue 4, pp. 203-9, 2002

Rowles, Scherer, Xi, Majer, Nickle, Rommens, Popov, Harris, Riebow, Xia, Tsui, Bogardus, Prochazka: "Cloning and characterization of PDK4 on 7q21.3 encoding a fourth pyruvate dehydrogenase kinase isoenzyme in human." in: The Journal of biological chemistry, Vol. 271, Issue 37, pp. 22376-82, 1996

Gudi, Bowker-Kinley, Kedishvili, Zhao, Popov: "Diversity of the pyruvate dehydrogenase kinase gene family in humans." in: The Journal of biological chemistry, Vol. 270, Issue 48, pp. 28989-94, 1996

Images

Product Details anti-PDK4 Antibody Target Details PDK4 Application Details Handling References for anti-PDK4 Antibody (ABIN359100) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PDK4 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 4) (Middle Region) (ABIN359100) Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with PDK4 a...
Western Blotting (WB) image for anti-PDK4 antibody (Pyruvate Dehydrogenase Kinase, Isozyme 4) (Middle Region) (ABIN359100) Western blot analysis of anti-PDK4 Antibody (Center) in HL60 cell line lysates (35ug/...