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|Application / Reactivity||Human|
|Dot Blot (DB)||11 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies|
|Immunofluorescence (IF)||24 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||77 Antibodies|
|Immunohistochemistry (IHC)||55 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||42 Antibodies|
|Immunoprecipitation (IP)||11 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Western Blotting (WB)||130 Antibodies|
|Antigen||3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies|
|Conjugate||This PDPK1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA)
|5 references available|
|Supplier||Log in to see|
Product Details anti-PDPK1 AntibodyTarget Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN358390) Images
|Specificity||This antibody detects PDPK1 pSer396.|
|Purification||Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S396 of human PDPK1.|
Target Details PDPK1Product Details anti-PDPK1 Antibody Application Details Handling References for anti-PDPK1 Antibody (ABIN358390) Images back to top
|Alternative Name||PDPK1 (PDPK1 Antibody Abstract)|
|Background||PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.Synonyms: 3-phosphoinositide-dependent proteinkinase 1, PDK-1, PDK1, PDPK1, hPDK1|
|Molecular Weight||63151 Da|
|Gene ID||5170, 9606|
|Pathways||PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-PDPK1 Antibody Target Details PDPK1 Handling References for anti-PDPK1 Antibody (ABIN358390) Images back to top
ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PDPK1 Antibody Target Details PDPK1 Application Details References for anti-PDPK1 Antibody (ABIN358390) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-PDPK1 Antibody (ABIN358390)Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Nilsen, Slagsvold, Skjerpen et al.: "Peroxisomal targeting as a tool for assaying potein-protein interactions in the living cell: cytokine-independent survival kinase (CISK) binds PDK-1 in vivo in a phosphorylation-dependent manner." in: The Journal of biological chemistry, Vol. 279, Issue 6, pp. 4794-801, 2004 (PubMed).
Collins, Deak, Arthur et al.: "In vivo role of the PIF-binding docking site of PDK1 defined by knock-in mutation." in: The EMBO journal, Vol. 22, Issue 16, pp. 4202-11, 2003 (PubMed).
Scott, Ingram, Ball: "PDK1-dependent activation of atypical PKC leads to degradation of the p21 tumour modifier protein." in: The EMBO journal, Vol. 21, Issue 24, pp. 6771-80, 2002 (PubMed).
Sato, Fujita, Tsuruo: "Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39360-7, 2002 (PubMed).
Egawa, Maegawa, Shi et al.: "Membrane localization of 3-phosphoinositide-dependent protein kinase-1 stimulates activities of Akt and atypical protein kinase C but does not stimulate glucose transport and glycogen synthesis in ..." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38863-9, 2002 (PubMed).
ImagesProduct Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN358390) back to top