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anti-Human PDPK1 antibody for Enzyme Immunoassay

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • 1210
  • BG02759
  • CD79B
  • CG1201
  • CG1210
  • Dmel\\CG1210
  • dPDK-1
  • dPDK1
  • Dstpk61
  • DSTPK61
  • dSTPK61
  • LOC100304995
  • MGC82080
  • PDK
  • PDK-1
  • PDK1
  • Pdk1
  • PDK1/Pk61C
  • pdpk1
  • PDPK2
  • Pk61C
  • pk61c
  • PK61C
  • Pk61C/PDK1
  • PRO0461
  • zgc:153787
Epitope
pSer396
49
20
18
13
13
13
13
6
6
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
248
150
148
7
6
4
4
2
1
1
1
1
1
Host
Rabbit
220
30
1
Clonality
Polyclonal
Conjugate
This PDPK1 antibody is un-conjugated
9
9
8
8
8
7
7
7
7
7
7
7
2
1
1
Application
Enzyme Immunoassay (EIA)
133
77
65
56
42
24
12
11
5
3
3
1
1
1
Supplier
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Catalog No. ABIN358390
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088776264 ABIN954076 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.00088776264 ABIN303441 EIA IHC (p) Rabbit AA 236-248, pSer241 Log in to see Polyclonal
0.00088776264 ABIN2728601 EIA Rabbit IgG Log in to see Polyclonal

Top referenced anti-PDPK1 antibody for Enzyme Immunoassay

  • Human Polyclonal PDPK1 Primary Antibody for EIA - ABIN358390 (5 Publications): Nilsen, Slagsvold, Skjerpen, Brech, Stenmark, Olsnes: Peroxisomal targeting as a tool for assaying potein-protein interactions in the living cell: cytokine-independent survival kinase (CISK) binds PDK-1 in vivo in a phosphorylation-dependent manner. in The Journal of biological chemistry 2004 (PubMed)

Similar anti-PDPK1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 11 Antibodies
ELISA 65 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 26 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 77 Antibodies
Immunohistochemistry (IHC) 55 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 140 Antibodies

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Epitope pSer396
(49), (20), (18), (13), (13), (13), (13), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(248), (150), (148), (7), (6), (4), (4), (2), (1), (1), (1), (1), (1)
Host Rabbit
(220), (30), (1)
Clonality
Polyclonal
Conjugate This PDPK1 antibody is un-conjugated
(9), (9), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (2), (1), (1)
Application Enzyme Immunoassay (EIA)
(133), (77), (65), (56), (42), (24), (12), (11), (5), (3), (3), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN358390) Images
Specificity This antibody detects PDPK1 pSer396.
Purification Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S396 of human PDPK1.
Isotype Ig

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling References for anti-PDPK1 Antibody (ABIN358390) Images back to top
Antigen
Alternative Name PDPK1 (PDPK1 Antibody Abstract)
Background PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.Synonyms: 3-phosphoinositide-dependent proteinkinase 1, PDK-1, PDK1, PDPK1, hPDK1
Molecular Weight 63151 Da
Gene ID 5170, 9606
UniProt O15530
Research Area Kinases/Phosphatases
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling References for anti-PDPK1 Antibody (ABIN358390) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details References for anti-PDPK1 Antibody (ABIN358390) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PDPK1 Antibody (ABIN358390)

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Background publications

Nilsen, Slagsvold, Skjerpen, Brech, Stenmark, Olsnes: "Peroxisomal targeting as a tool for assaying potein-protein interactions in the living cell: cytokine-independent survival kinase (CISK) binds PDK-1 in vivo in a phosphorylation-dependent manner." in: The Journal of biological chemistry, Vol. 279, Issue 6, pp. 4794-801, 2004

Collins, Deak, Arthur, Armit, Alessi: "In vivo role of the PIF-binding docking site of PDK1 defined by knock-in mutation." in: The EMBO journal, Vol. 22, Issue 16, pp. 4202-11, 2003

Egawa, Maegawa, Shi, Nakamura, Obata, Yoshizaki, Morino, Shimizu, Nishio, Suzuki, Kashiwagi et al.: "Membrane localization of 3-phosphoinositide-dependent protein kinase-1 stimulates activities of Akt and atypical protein kinase C but does not stimulate glucose transport and glycogen synthesis in ..." in: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38863-9, 2002

Sato, Fujita, Tsuruo: "Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39360-7, 2002

Scott, Ingram, Ball: "PDK1-dependent activation of atypical PKC leads to degradation of the p21 tumour modifier protein." in: The EMBO journal, Vol. 21, Issue 24, pp. 6771-80, 2002

Images

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN358390) back to top
Supplier Images
 image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pSer396) (ABIN358390) anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer396) antibody