anti-Human PDPK1 antibody for Immunocytochemistry

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • pdpk1
  • MGC82080
  • PDK1
  • PDPK2
  • PRO0461
  • Pdk1
  • zgc:153787
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • 3-phosphoinositide dependent protein kinase-1
  • 3-phosphoinositide dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase-1a
  • 3-phosphoinositide-dependent protein kinase 1
  • Phosphoinositide-dependent kinase 1
  • Protein PDK-1
  • PDPK1
  • pdpk1
  • Pdpk1
  • pdpk1a
  • Pdk1
  • pdk-1
Reactivity
Human, Monkey, Rat (Rattus)
464
221
220
18
13
12
11
11
6
5
4
3
2
1
1
1
1
1
1
1
Host
Mouse
384
72
16
Clonality
Monoclonal
Conjugate
This PDPK1 antibody is un-conjugated
19
17
16
11
11
7
7
7
7
7
7
7
7
7
7
6
5
5
4
4
3
3
3
2
2
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
313
157
101
91
67
66
39
30
22
10
7
5
3
2
1
Options
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Catalog No. ABIN969347
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.375908 ABIN5542260 ELISA FACS ICC IHC WB Mouse IgG1 AA 457-556 Log in to see 3H3D9
26.375908 ABIN5542259 ELISA FACS ICC IHC WB Mouse IgG1 AA 457-556 Log in to see 7E4G11
1 ABIN250776 ICC IF WB Rabbit C-Term, AA 350-436 Log in to see Polyclonal 3
1 ABIN450008 ELISA FACS ICC IF WB Mouse IgG1 kappa AA 29-436 Log in to see 2D6
1 ABIN4344570 ICC IF IHC IHC (p) WB Rabbit pSer241 Log in to see Polyclonal
1 ABIN4344573 ICC IF IP WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN1860177 ICC IHC IP WB Rabbit IgG AA 83-338 Log in to see Polyclonal
1 ABIN4880931 ELISA FACS ICC IHC WB Mouse IgG1 AA 457-556 Log in to see 7E4G11
1 ABIN4880932 ELISA FACS ICC WB Mouse IgG1 Log in to see 3H3D9
1 ABIN5078734 ICC IF Rabbit IgG Log in to see Polyclonal
1 ABIN4344574 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4A11
1 ABIN4344641 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal
1 ABIN4880420 ELISA FACS ICC IHC WB Mouse IgG1 Log in to see 4A11
1 ABIN5078729 ICC IF Rabbit IgG Log in to see Polyclonal
1 ABIN4344595 ELISA FACS ICC IF WB DyLight 405 Mouse IgG1 kappa AA 29-436 Log in to see 2D6
1 ABIN4344586 ELISA FACS ICC IF WB DyLight 650 Mouse IgG1 kappa AA 29-436 Log in to see 2D6
1 ABIN4344587 ELISA FACS ICC IF FITC Mouse IgG1 kappa AA 29-436 Log in to see 2D6
1 ABIN4344589 ELISA FACS ICC IF WB DyLight 488 Mouse IgG1 kappa AA 29-436 Log in to see 2D6
1 ABIN4344591 ELISA FACS ICC IF WB PerCP Mouse IgG1 kappa AA 29-436 Log in to see 2D6
1 ABIN4344580 ELISA FACS ICC IF WB Alexa Fluor 405 Mouse IgG1 kappa AA 29-436 Log in to see 2D6

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Reactivity Human, Monkey, Rat (Rattus)
(464), (221), (220), (18), (13), (12), (11), (11), (6), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(384), (72), (16)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PDPK1 antibody is un-conjugated
(19), (17), (16), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(313), (157), (101), (91), (67), (66), (39), (30), (22), (10), (7), (5), (3), (2), (1)
Pubmed 3 references available
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Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling References for anti-PDPK1 antibody (ABIN969347) Images
Purification Ascites
Immunogen Purified recombinant fragment of human PDK1 expressed in E. Coli.
Clone 4A11
Isotype IgG1

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling References for anti-PDPK1 antibody (ABIN969347) Images back to top
Antigen
Alternative Name PDK1 (PDPK1 Antibody Abstract)
Background Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. (provided by RefSeq). Tissue specificity: Expressed predominantly in the heart.
Molecular Weight 44 kDa
Gene ID 5163
HGNC 5163
Research Area Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling References for anti-PDPK1 antibody (ABIN969347) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details References for anti-PDPK1 antibody (ABIN969347) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-PDPK1 antibody (ABIN969347)

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Product cited in:

Weisberg, Banerji, Wright, Barrett, Ray, Moreno, Catley, Jiang, Hall-Meyers, Sauveur-Michel, Stone, Galinsky, Fox, Kung, Griffin: "Potentiation of antileukemic therapies by the dual PI3K/PDK-1 inhibitor, BAG956: effects on BCR-ABL- and mutant FLT3-expressing cells." in: Blood, Vol. 111, Issue 7, pp. 3723-34, 2008

Pinner, Sahai: "PDK1 regulates cancer cell motility by antagonising inhibition of ROCK1 by RhoE." in: Nature cell biology, Vol. 10, Issue 2, pp. 127-37, 2008

Seong, Jung, Kim, Ha: "3-Phosphoinositide-dependent PDK1 negatively regulates transforming growth factor-beta-induced signaling in a kinase-dependent manner through physical interaction with Smad proteins." in: The Journal of biological chemistry, Vol. 282, Issue 16, pp. 12272-89, 2007

Images

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling References for anti-PDPK1 antibody (ABIN969347) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibody (ABIN969347) Figure 1: Flow cytometric analysis of Lovo cells using PDK1 mouse mAb (green) and neg...
Immunocytochemistry (ICC) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibody (ABIN969347) Figure 2: Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green) . Bl...
Immunohistochemistry (IHC) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibody (ABIN969347) Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (le...
Western Blotting (WB) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) antibody (ABIN969347) Figure 4: Western blot analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), J...