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anti-Human PDPK1 antibody for Immunocytochemistry

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • 1210
  • BG02759
  • CD79B
  • CG1201
  • CG1210
  • Dmel\\CG1210
  • dPDK-1
  • dPDK1
  • Dstpk61
  • DSTPK61
  • dSTPK61
  • LOC100304995
  • MGC82080
  • PDK
  • PDK-1
  • PDK1
  • Pdk1
  • PDK1/Pk61C
  • pdpk1
  • PDPK2
  • Pk61C
  • pk61c
  • PK61C
  • Pk61C/PDK1
  • PRO0461
  • zgc:153787
Epitope
C-Term, AA 350-436
49
20
19
13
13
13
13
6
6
5
4
4
4
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
249
150
148
7
6
4
4
2
1
1
1
1
1
Host
Rabbit
221
30
1
Clonality
Polyclonal
Conjugate
This PDPK1 antibody is un-conjugated
9
9
8
8
8
7
7
7
7
7
7
7
2
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
133
77
65
56
42
24
12
11
5
4
3
1
1
1
Supplier
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Available images

Catalog No. ABIN407052
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010161072 ABIN2737344 ICC IP IHC WB Rabbit IgG pSer241 Log in to see Polyclonal
0.0010161072 ABIN1860177 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 83-338 Log in to see Polyclonal
0.0010161072 ABIN450008 ELISA FACS ICC IF WB Mouse IgG1 kappa AA 29-436 Log in to see 2D6
0.0010161072 ABIN2988237 ICC IF IHC WB Rabbit IgG pSer241 Log in to see Polyclonal
0.0010161072 ABIN2990094 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010161072 ABIN3059719 ICC IF IP IHC WB Rabbit pSer241 Log in to see Polyclonal

Similar anti-PDPK1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 11 Antibodies
ELISA 65 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Immunocytochemistry (ICC) 7 Antibodies
Immunofluorescence (IF) 26 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 77 Antibodies
Immunohistochemistry (IHC) 55 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 140 Antibodies

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Epitope C-Term, AA 350-436
(49), (20), (19), (13), (13), (13), (13), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(249), (150), (148), (7), (6), (4), (4), (2), (1), (1), (1), (1), (1)
Host Rabbit
(221), (30), (1)
Clonality
Polyclonal
Conjugate This PDPK1 antibody is un-conjugated
(9), (9), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (2), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(133), (77), (65), (56), (42), (24), (12), (11), (5), (4), (3), (1), (1), (1)
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Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling Images
Cross-Reactivity (Details) Predicted to react with mouse,rat,based on 100% sequence homology.
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to a C-terminal portion of the human PDK1 protein sequence (between residues 350-436). [Swiss-Prot Q15118]

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PDK-1 (PDPK1 Antibody Abstract)
Background Gene Symbol: PDK1
Molecular Weight Theoretical MW: 49 kDa
UniProt Q15118
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling Images back to top
Application Notes Western Blot 2 μg/mL, Immunocytochemistry/Immunofluorescence 1:1000-1:2000
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for PDK1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 1 hour at room temperature (RT).
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-PDK1 primary antibody in blocking buffer and incubate 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (we used BioFX Super Plus ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Images back to top
Format Liquid
Concentration 1.42 mg/mL
Buffer Tris-glycine, 150 mM NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Images

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (C-Term) (ABIN407052) Detection of PDK1 in human heart lysate using ABIN250776 at 2 ug/ml.