You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human PDPK1 antibody for Immunocytochemistry

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • 1210
  • BG02759
  • CD79B
  • CG1201
  • CG1210
  • Dmel\\CG1210
  • dPDK-1
  • dPDK1
  • Dstpk61
  • DSTPK61
  • dSTPK61
  • LOC100304995
  • MGC82080
  • PDK
  • PDK-1
  • PDK1
  • Pdk1
  • PDK1/Pk61C
  • pdpk1
  • PDPK2
  • Pk61C
  • pk61c
  • PK61C
  • Pk61C/PDK1
  • PRO0461
  • zgc:153787
Reactivity
Human, Monkey, Rat (Rattus)
424
188
185
10
9
8
7
6
4
1
1
1
1
1
1
1
1
Host
Mouse
346
69
15
Clonality
Monoclonal
Conjugate
This PDPK1 antibody is un-conjugated
17
15
14
12
11
11
7
7
7
7
7
7
6
5
5
4
4
3
3
3
3
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
282
153
99
84
58
55
36
27
17
11
7
3
2
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN969347
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.60702187 ABIN1844865 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see
0.0010101626 ABIN250776 ICC IF WB Rabbit C-Term, AA 350-436 Log in to see Polyclonal 3
0.0010101626 ABIN4880931 ELISA FACS ICC IHC WB Mouse IgG1 AA 457-556 Log in to see 7E4G11
0.0010101626 ABIN4880932 ELISA FACS ICC WB Mouse IgG1 Log in to see 3H3D9
0.0010101626 ABIN1860177 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 83-338 Log in to see Polyclonal
0.0010101626 ABIN4880420 ELISA FACS ICC IHC WB Mouse IgG1 Log in to see 4A11
0.0010101626 ABIN2737344 ICC IP IHC WB Rabbit IgG pSer241 Log in to see Polyclonal
0.0010101626 ABIN450008 ELISA FACS ICC IF WB Mouse IgG1 kappa AA 29-436 Log in to see 2D6
0.0010101626 ABIN4344580 ELISA FACS ICC IF WB Alexa Fluor 405 Mouse IgG1 kappa AA 29-436 Log in to see 2D6
0.0010101626 ABIN4344581 ELISA FACS ICC IF WB Alexa Fluor 488 Mouse IgG1 kappa AA 29-436 Log in to see 2D6
0.0010101626 ABIN4344582 ELISA FACS ICC IF WB Alexa Fluor 647 Mouse IgG1 kappa AA 29-436 Log in to see 2D6
0.0010101626 ABIN4344583 ELISA FACS ICC IF WB Alexa Fluor 700 Mouse IgG1 kappa AA 29-436 Log in to see 2D6
0.0010101626 ABIN4344584 ELISA FACS ICC IF WB Allophycocyanin Mouse IgG1 kappa AA 29-436 Log in to see 2D6
0.0010101626 ABIN4344585 ELISA FACS ICC IF WB Biotin Mouse IgG1 kappa AA 29-436 Log in to see 2D6
0.0010101626 ABIN4344586 ELISA FACS ICC IF WB DyLight 650 Mouse IgG1 kappa AA 29-436 Log in to see 2D6
0.0010101626 ABIN4344588 ELISA FACS ICC IF WB DyLight 680 Mouse IgG1 kappa AA 29-436 Log in to see 2D6
0.0010101626 ABIN4344589 ELISA FACS ICC IF WB DyLight 488 Mouse IgG1 kappa AA 29-436 Log in to see 2D6
0.0010101626 ABIN4344592 ELISA FACS ICC IF WB PE Mouse IgG1 kappa AA 29-436 Log in to see 2D6
0.0010101626 ABIN4344591 ELISA FACS ICC IF WB PerCP Mouse IgG1 kappa AA 29-436 Log in to see 2D6
0.0010101626 ABIN4344587 ELISA FACS ICC IF WB FITC Mouse IgG1 kappa AA 29-436 Log in to see 2D6

Top referenced anti-PDPK1 antibody for Immunocytochemistry

Similar anti-PDPK1 Antibodies

Application / Reactivity Rat (Rattus) Monkey Human
ELISA 36 Antibodies 7 Antibodies 152 Antibodies
Flow Cytometry (FACS) 8 Antibodies 7 Antibodies
Immunocytochemistry (ICC) 10 Antibodies 4 Antibodies
Immunofluorescence (IF) 21 Antibodies 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 84 Antibodies
Immunohistochemistry (IHC) 54 Antibodies 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 30 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 95 Antibodies 10 Antibodies
Dot Blot (DB) 11 Antibodies
ELISA (Capture) 2 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Reactivity Human, Monkey, Rat (Rattus)
(424), (188), (185), (10), (9), (8), (7), (6), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(346), (69), (15)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PDPK1 antibody is un-conjugated
(17), (15), (14), (12), (11), (11), (7), (7), (7), (7), (7), (7), (6), (5), (5), (4), (4), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(282), (153), (99), (84), (58), (55), (36), (27), (17), (11), (7), (3), (2), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN969347) Images
Purification Ascites
Immunogen Purified recombinant fragment of human PDK1 expressed in E. coli.
Clone 4A11
Isotype IgG1

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling References for anti-PDPK1 Antibody (ABIN969347) Images back to top
Antigen
Alternative Name PDK1 (PDPK1 Antibody Abstract)
Background Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. (provided by RefSeq). Tissue specificity: Expressed predominantly in the heart.
Molecular Weight 44 kDa
Gene ID 5163
HGNC 5163
Research Area Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling References for anti-PDPK1 Antibody (ABIN969347) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details References for anti-PDPK1 Antibody (ABIN969347) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-PDPK1 Antibody (ABIN969347)

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Product cited in:

Weisberg, Banerji, Wright, Barrett, Ray, Moreno, Catley, Jiang, Hall-Meyers, Sauveur-Michel, Stone, Galinsky, Fox, Kung, Griffin: "Potentiation of antileukemic therapies by the dual PI3K/PDK-1 inhibitor, BAG956: effects on BCR-ABL- and mutant FLT3-expressing cells." in: Blood, Vol. 111, Issue 7, pp. 3723-34, 2008

Pinner, Sahai: "PDK1 regulates cancer cell motility by antagonising inhibition of ROCK1 by RhoE." in: Nature cell biology, Vol. 10, Issue 2, pp. 127-37, 2008

Seong, Jung, Kim, Ha: "3-Phosphoinositide-dependent PDK1 negatively regulates transforming growth factor-beta-induced signaling in a kinase-dependent manner through physical interaction with Smad proteins." in: The Journal of biological chemistry, Vol. 282, Issue 16, pp. 12272-89, 2007

Images

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN969347) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (ABIN969347) Figure 1: Flow cytometric analysis of Lovo cells using PDK1 mouse mAb (green) and neg...
Immunocytochemistry (ICC) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (ABIN969347) Figure 2: Immunofluorescence analysis of HELA cells using PDK1 mouse mAb (green) . Bl...
Immunohistochemistry (IHC) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (ABIN969347) Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues (le...
Western Blotting (WB) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (ABIN969347) Figure 4: Western blot analysis using PDK1 mouse mAb against NIH/3T3 (1), Hela (2), J...