You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human PDPK1 antibody for Immunofluorescence

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • 1210
  • BG02759
  • CD79B
  • CG1201
  • CG1210
  • Dmel\\CG1210
  • dPDK-1
  • dPDK1
  • Dstpk61
  • DSTPK61
  • dSTPK61
  • LOC100304995
  • MGC82080
  • PDK
  • PDK-1
  • PDK1
  • Pdk1
  • PDK1/Pk61C
  • pdpk1
  • PDPK2
  • Pk61C
  • pk61c
  • PK61C
  • Pk61C/PDK1
  • PRO0461
  • zgc:153787
Epitope
C-Term, AA 350-436
50
20
19
19
13
13
13
13
13
11
8
8
6
6
5
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
293
153
152
7
6
4
4
3
2
1
1
1
1
1
1
Host
Rabbit
243
52
1
Clonality
Polyclonal
Conjugate
This PDPK1 antibody is un-conjugated
12
11
11
10
9
7
7
7
7
7
7
7
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
176
88
77
63
51
46
35
22
12
11
4
3
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN250776
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.28989956 ABIN1870500 IF IHC WB Rabbit IgG pSer241 Log in to see Polyclonal
0.28989956 ABIN2404484 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.28989956 ABIN4344570 ICC IF IHC IHC (p) WB Rabbit pSer241 Log in to see Polyclonal
0.28989956 ABIN3020427 IF IHC WB Rabbit pSer241 Log in to see Polyclonal
0.28989956 ABIN4344574 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4A11
0.28989956 ABIN4344573 ICC IF WB Rabbit Log in to see Polyclonal
0.28989956 ABIN4344641 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal
0.0010991256 ABIN196616 IF IHC (p) WB Rabbit pSer241 Log in to see Polyclonal 4
0.0010991256 ABIN197100 IF IHC (p) WB Rabbit Ser241 Log in to see Polyclonal 5
0.0010991256 ABIN968351 IF WB Mouse IgG1 AA 439-555 Log in to see 5-PDK1 5
0.0010991256 ABIN968352 IF WB Mouse IgG1 AA 439-555 Log in to see 5-PDK1 5
0.0010991256 ABIN362492 IF IHC WB Rabbit IgG AA 239-243 Log in to see Polyclonal 6
0.0010991256 ABIN361882 IF IHC WB Rabbit IgG pSer241 Log in to see Polyclonal 6
0.0010991256 ABIN602119 IF IHC WB Rabbit IgG pSer241 Log in to see Polyclonal
0.0010991256 ABIN2882761 IF Rabbit IgG pSer241 Log in to see Polyclonal
0.0010991256 ABIN450008 ELISA FACS ICC IF WB Mouse IgG1 kappa AA 29-436 Log in to see 2D6
0.0010991256 ABIN2879850 IF Rabbit IgG Log in to see Polyclonal
0.0010991256 ABIN2332580 IF IHC WB Rabbit IgG pSer241 Log in to see Polyclonal
0.0010991256 ABIN2699627 IF IHC WB Rabbit IgG pSer241 Log in to see Polyclonal
0.0010991256 ABIN2887016 IF Rabbit Log in to see Polyclonal

Top referenced anti-PDPK1 antibody for Immunofluorescence

Similar anti-PDPK1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 11 Antibodies
ELISA 88 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Immunocytochemistry (ICC) 37 Antibodies
Immunofluorescence (IF) 53 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 77 Antibodies
Immunohistochemistry (IHC) 62 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 46 Antibodies
Immunoprecipitation (IP) 11 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 183 Antibodies

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Epitope C-Term, AA 350-436
(50), (20), (19), (19), (13), (13), (13), (13), (13), (11), (8), (8), (6), (6), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(293), (153), (152), (7), (6), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(243), (52), (1)
Clonality
Polyclonal
Conjugate This PDPK1 antibody is un-conjugated
(12), (11), (11), (10), (9), (7), (7), (7), (7), (7), (7), (7), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(176), (88), (77), (63), (51), (46), (35), (22), (12), (11), (4), (3), (1), (1), (1)
Pubmed 3 references available
Supplier Log in to see

Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN250776) Images
Cross-Reactivity (Details) Predicted to react with mouse,rat,based on 100% sequence homology.
Purification Immunogen affinity purified
Immunogen A synthetic peptide made to a C-terminal portion of the human PDK1 protein sequence (between residues 350-436). [Swiss-Prot Q15118]

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling References for anti-PDPK1 Antibody (ABIN250776) Images back to top
Antigen
Alternative Name PDK-1 (PDPK1 Antibody Abstract)
Background Gene Symbol: PDK1
Molecular Weight Theoretical MW: 49 kDa
UniProt Q15118
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling References for anti-PDPK1 Antibody (ABIN250776) Images back to top
Application Notes Western Blot 2 μg/mL, Immunocytochemistry/Immunofluorescence 1:1000-1:2000
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for PDK1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 1 hour at room temperature (RT).
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-PDK1 primary antibody in blocking buffer and incubate 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (we used BioFX Super Plus ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details References for anti-PDPK1 Antibody (ABIN250776) Images back to top
Format Liquid
Concentration 1.42 mg/mL
Buffer Tris-glycine, 150 mM NaCl
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C,-80 °C
Storage Comment Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

References for anti-PDPK1 Antibody (ABIN250776)

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Product cited in:

Tsoi, Li, Chen, Lau, Tsui, Chan: "The SARS-coronavirus membrane protein induces apoptosis via interfering with PDK1-PKB/Akt signalling." in: The Biochemical journal, Vol. 464, Issue 3, pp. 439-47, 2014 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Luo, Chang, Zhong, Pandey, Semenza: "Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 49, pp. E3367-76, 2012

Luo, Hu, Chang, Zhong, Knabel, OMeally, Cole, Pandey, Semenza: "Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1." in: Cell, Vol. 145, Issue 5, pp. 732-44, 2011

Images

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN250776) back to top
Supplier Images
Western Blotting (WB) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (C-Term) (ABIN250776) Detection of PDK1 in human heart lysate using ABIN250776 at 2 ug/ml.
Western Blotting (WB) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (C-Term) (ABIN250776) Western Blot: PDK-1 Antibody [ABIN250776] - Analysis of HeLa lysates using ABIN250776...
Immunofluorescence (IF) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (C-Term) (ABIN250776) Immunocytochemistry/Immunofluorescence: PDK-1 Antibody [ABIN250776] - Antibody was te...