anti-Human PDPK1 antibody for Immunohistochemistry

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • pdpk1
  • MGC82080
  • PDK1
  • PDPK2
  • PRO0461
  • Pdk1
  • zgc:153787
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • 3-phosphoinositide dependent protein kinase-1
  • 3-phosphoinositide dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase-1a
  • 3-phosphoinositide-dependent protein kinase 1
  • Phosphoinositide-dependent kinase 1
  • Protein PDK-1
  • PDPK1
  • pdpk1
  • Pdpk1
  • pdpk1a
  • Pdk1
  • pdk-1
Epitope
pTyr9
59
35
32
27
25
21
18
17
15
15
15
15
8
6
6
6
6
5
5
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
459
220
220
19
13
12
11
11
6
5
4
3
2
1
1
1
1
1
1
1
Host
Rabbit
378
72
16
Clonality
Polyclonal
Conjugate
This PDPK1 antibody is un-conjugated
19
17
16
11
11
7
7
7
7
7
7
7
7
7
7
6
5
5
4
4
3
3
3
2
2
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA
308
156
96
91
67
63
40
30
20
10
7
5
3
2
1
Options
Supplier
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Catalog No. ABIN1532025
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.637035 ABIN969347 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 4A11 3
9.637035 ABIN1531949 IHC ELISA WB Rabbit IgG pSer241 Log in to see Polyclonal
9.637035 ABIN1532981 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
9.637035 ABIN2706787 IP IHC WB Rabbit pSer241 Log in to see Polyclonal
9.637035 ABIN1870500 IF IHC WB Rabbit IgG pSer241 Log in to see Polyclonal
9.637035 ABIN1874088 IF IHC WB Rabbit IgG Log in to see Polyclonal
9.637035 ABIN2470411 IHC ELISA Rabbit AA 236-248, pSer241 Log in to see Polyclonal
9.637035 ABIN2404484 IF IHC WB Rabbit IgG Log in to see Polyclonal
9.637035 ABIN1844865 ELISA FACS IF/ICC IHC WB Mouse IgG1 Log in to see
9.637035 ABIN3020427 IF IHC WB Rabbit pSer241 Log in to see Polyclonal
9.637035 ABIN5542259 ELISA FACS ICC IHC WB Mouse IgG1 AA 457-556 Log in to see 7E4G11
9.637035 ABIN3022352 IHC WB Rabbit Log in to see Polyclonal
9.637035 ABIN1847564 IHC ELISA Rabbit N-Term, pTyr9 Log in to see Polyclonal
1 ABIN4344572 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN361882 IF IHC WB Rabbit IgG pSer241 Log in to see Polyclonal 6
1 ABIN223167 IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN362492 IF IHC WB Rabbit IgG AA 239-243 Log in to see Polyclonal 6
1 ABIN3186376 ELISA IHC WB Rabbit IgG Thr222 Log in to see Polyclonal
1 ABIN105431 IP IHC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN4880931 ELISA FACS ICC IHC WB Mouse IgG1 AA 457-556 Log in to see 7E4G11

Top referenced anti-PDPK1 antibody for Immunohistochemistry

Similar anti-PDPK1 Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine)
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies 4 Antibodies 3 Antibodies
Western Blotting (WB) 301 Antibodies 116 Antibodies 114 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies 3 Antibodies
Immunoprecipitation (IP) 19 Antibodies 13 Antibodies 10 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 67 Antibodies 39 Antibodies 42 Antibodies
Immunohistochemistry (IHC) 96 Antibodies 54 Antibodies 57 Antibodies

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Epitope pTyr9
(59), (35), (32), (27), (25), (21), (18), (17), (15), (15), (15), (15), (8), (6), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(459), (220), (220), (19), (13), (12), (11), (11), (6), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(378), (72), (16)
Clonality
Polyclonal
Conjugate This PDPK1 antibody is un-conjugated
(19), (17), (16), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA
(308), (156), (96), (91), (67), (63), (40), (30), (20), (10), (7), (5), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling ProductDetails: References for anti-PDPK1 antibody (ABIN1532025) Images
Specificity PDK1 (Phospho-Tyr9) Antibody detects endogenous levels of PDK1 only when phosphorylated at Tyr9.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human PDK1 around the phosphorylation site of Tyr9.
Isotype IgG

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling ProductDetails: References for anti-PDPK1 antibody (ABIN1532025) Images back to top
Antigen
Alternative Name PDK1 (PDPK1 Antibody Abstract)
Background Molecular weight of target protein: 63 kDa
Synonyms: 3-phosphoinositide dependent protein kinase-1, hPDK1, kinase PDK1, mPDK1, PDPK1, PkB kinase, Protein kinase B kinase
Molecular Weight 63 kDa
Gene ID 5170
OMIM 605213
UniProt O15530
Research Area Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling ProductDetails: References for anti-PDPK1 antibody (ABIN1532025) Images back to top
Application Notes IHC: 1:50-1:100
ELISA: 1:40000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.459691 (NCBI Gene Symbol: PDPK1)

Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details ProductDetails: References for anti-PDPK1 antibody (ABIN1532025) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

ProductDetails: References for anti-PDPK1 antibody (ABIN1532025)

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Background publications

Stephens, Anderson, Stokoe, Erdjument-Bromage, Painter, Holmes, Gaffney, Reese, McCormick, Tempst, Coadwell, Hawkins: "Protein kinase B kinases that mediate phosphatidylinositol 3,4,5-trisphosphate-dependent activation of protein kinase B." in: Science (New York, N.Y.), Vol. 279, Issue 5351, pp. 710-4, 1998

Images

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling ProductDetails: References for anti-PDPK1 antibody (ABIN1532025) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pTyr9) (ABIN1532025) Immunohistochemistry analysis of paraffin-embedded human brain, using PDK1 (Phospho-T...