anti-Human PDPK1 antibody for Western Blotting

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • pdpk1
  • MGC82080
  • PDK1
  • PDPK2
  • PRO0461
  • Pdk1
  • zgc:153787
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • 3-phosphoinositide dependent protein kinase-1
  • 3-phosphoinositide dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase-1a
  • 3-phosphoinositide-dependent protein kinase 1
  • Phosphoinositide-dependent kinase 1
  • Protein PDK-1
  • PDPK1
  • pdpk1
  • Pdpk1
  • pdpk1a
  • Pdk1
  • pdk-1
Epitope
Middle Region
60
35
32
27
27
26
18
17
15
15
15
15
8
7
6
6
6
5
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Yeast
465
220
220
19
13
11
10
10
6
4
3
3
2
1
1
1
1
1
1
Host
Rabbit
383
73
16
Clonality
Polyclonal
Conjugate
This PDPK1 antibody is un-conjugated
19
17
16
11
11
7
7
7
7
7
7
7
7
7
7
6
5
5
4
4
3
3
3
2
2
1
1
1
1
1
1
Application
Western Blotting (WB)
313
158
102
91
67
66
40
31
22
10
7
5
3
2
1
Options
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Catalog No. ABIN2777432
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9060245 ABIN2779320 WB Rabbit Middle Region Log in to see Polyclonal 1
2.9060245 ABIN1533064 ELISA WB Rabbit IgG Log in to see Polyclonal 1
2.9060245 ABIN250776 ICC IF WB Rabbit C-Term, AA 350-436 Log in to see Polyclonal 3
2.9060245 ABIN3043600 IHC (p) WB Rabbit IgG AA 524-556, C-Term Log in to see Polyclonal
2.9060245 ABIN3044043 IHC (p) WB Rabbit IgG AA 408-421, C-Term Log in to see Polyclonal
2.9060245 ABIN250778 WB Rabbit AA 300-400, Internal Region Log in to see Polyclonal 3
2.9060245 ABIN744668 IF (p) IHC (p) WB Rabbit IgG pSer241 Log in to see Polyclonal 1
2.9060245 ABIN223167 IP IHC WB Rabbit IgG Log in to see Polyclonal
2.9060245 ABIN2706787 IP IHC WB Rabbit pSer241 Log in to see Polyclonal
2.9060245 ABIN2706784 WB Rabbit Center Log in to see Polyclonal
2.9060245 ABIN359095 EIA WB Rabbit Ig N-Term Log in to see Polyclonal 3
2.9060245 ABIN967612 WB Mouse IgG1, kappa pSer241 Log in to see J66-653-44-22 2
2.9060245 ABIN968351 IF WB Mouse IgG1 AA 439-555 Log in to see 5-PDK1 5
2.9060245 ABIN968352 IF WB Mouse IgG1 AA 439-555 Log in to see 5-PDK1 5
2.9060245 ABIN4344572 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
2.9060245 ABIN1531949 IHC ELISA WB Rabbit IgG pSer241 Log in to see Polyclonal
2.9060245 ABIN269845 ELISA WB Goat Log in to see Polyclonal 1
2.9060245 ABIN969347 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 4A11 3
2.9060245 ABIN1532981 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.9060245 ABIN2856238 WB Rabbit IgG Center Log in to see Polyclonal

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Epitope Middle Region
(60), (35), (32), (27), (27), (26), (18), (17), (15), (15), (15), (15), (8), (7), (6), (6), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Yeast
(465), (220), (220), (19), (13), (11), (10), (10), (6), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(383), (73), (16)
Clonality
Polyclonal
Conjugate This PDPK1 antibody is un-conjugated
(19), (17), (16), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(313), (158), (102), (91), (67), (66), (40), (31), (22), (10), (7), (5), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling ProductDetails: References for anti-PDPK1 antibody (ABIN2777432) Images
Blocking Peptide Blocking peptide for this product available: ABIN981538
Sequence ATMEHHANRG VYPPIQVHVT LGNEDLTVKM SDRGGGVPLR KIDRLFNYMY
Predicted Reactivity Cow: 86%, Dog: 79%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 86%, Rabbit: 86%, Rat: 86%, Yeast: 90%
Characteristics This is a rabbit polyclonal antibody against PDK1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human PDK1

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling ProductDetails: References for anti-PDPK1 antibody (ABIN2777432) Images back to top
Antigen
Alternative Name PDK1 (PDPK1 Antibody Abstract)
Background Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: -
Protein Interaction Partner: EGFR, PDK1, KAT2B, USP4, UBC, MYC, XRCC6, SGK1, DLAT, RPS6KB1, PDK2, PDHA1, YWHAQ, RPTOR, RALGDS, PKN2, PKN1, MTOR, AKT1,
Protein Size: 436
Molecular Weight 47 kDa
Gene ID 5163
NCBI Accession NM_002610, NP_002601
UniProt Q15118
Research Area Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling ProductDetails: References for anti-PDPK1 antibody (ABIN2777432) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 436 AA

Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details ProductDetails: References for anti-PDPK1 antibody (ABIN2777432) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-PDPK1 antibody (ABIN2777432)

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Background publications

Ohba, Watanabe, Murakami, Radovanovic, Iino, Ishida, Tosa, Ono, Ito: "Amlodipine inhibits cell proliferation via PKD1-related pathway." in: Biochemical and biophysical research communications, Vol. 369, Issue 2, pp. 376-81, 2008

Images

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling ProductDetails: References for anti-PDPK1 antibody (ABIN2777432) back to top
Supplier Images
Western Blotting (WB) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (Middle Region) antibody (ABIN2777432) Host: Rabbit Target Name: CHAD Sample Type: Human Adult Placenta Antibody Dilution: 1...
Western Blotting (WB) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (Middle Region) antibody (ABIN2777432) Host: Rabbit Target Name: PDK1 Sample Tissue: Mouse Testis Antibody Dilution: 1ug/ml
Western Blotting (WB) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (Middle Region) antibody (ABIN2777432) Host: Rabbit Target Name: PDK2 Sample Type: Human Jurkat Antibody Dilution: 1.0ug/ml ...
Western Blotting (WB) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (Middle Region) antibody (ABIN2777432) WB Suggested Anti-PDK1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Positiv...