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anti-Human PDPK1 antibody for Western Blotting

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • 1210
  • BG02759
  • CD79B
  • CG1201
  • CG1210
  • Dmel\\CG1210
  • dPDK-1
  • dPDK1
  • Dstpk61
  • DSTPK61
  • dSTPK61
  • LOC100304995
  • MGC82080
  • PDK
  • PDK-1
  • PDK1
  • Pdk1
  • PDK1/Pk61C
  • pdpk1
  • PDPK2
  • Pk61C
  • pk61c
  • PK61C
  • Pk61C/PDK1
  • PRO0461
  • zgc:153787
Epitope
Middle Region
49
20
19
13
13
13
13
6
6
5
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio)
248
149
147
6
6
4
4
2
1
1
1
1
Host
Rabbit
220
30
1
Clonality
Polyclonal
Conjugate
This PDPK1 antibody is un-conjugated
9
9
8
8
8
7
7
7
7
7
7
7
2
1
1
Application
Western Blotting (WB)
132
77
65
56
42
24
12
11
5
4
3
1
1
1
Supplier
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Catalog No. ABIN2779320
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12118237 ABIN1533064 ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.12118237 ABIN967612 WB Mouse IgG1, kappa pSer241 Log in to see J66-653-44-22 2
0.12118237 ABIN968351 IF WB Mouse IgG1 AA 439-555 Log in to see 5-PDK1 5
0.12118237 ABIN968352 IF WB Mouse IgG1 AA 439-555 Log in to see 5-PDK1 5
0.12118237 ABIN3043600 IHC (p) WB Rabbit IgG AA 524-556, C-Term Log in to see Polyclonal
0.12118237 ABIN1531949 IHC ELISA WB Rabbit IgG pSer241 Log in to see Polyclonal
0.12118237 ABIN954076 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.12118237 ABIN223167 IP IHC WB Rabbit IgG Log in to see Polyclonal
0.12118237 ABIN1532981 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.12118237 ABIN518765 PLA WB Mouse AA 1-429, full length Log in to see Polyclonal 1
0.12118237 ABIN2706784 WB Rabbit Center Log in to see Polyclonal
0.12118237 ABIN2706787 IP IHC WB Rabbit pSer241 Log in to see Polyclonal
0.12118237 ABIN1870500 IF IHC WB Rabbit IgG pSer241 Log in to see Polyclonal
0.12118237 ABIN2460187 ELISA WB Rabbit Log in to see Polyclonal
0.12118237 ABIN1539808 WB Mouse IgM Log in to see 340CT15-1-1
0.12118237 ABIN2499647 IHC WB Rabbit IgG Ser241 Log in to see Polyclonal
0.12118237 ABIN2499648 IHC WB Rabbit IgG pSer241 Log in to see Polyclonal
0.12118237 ABIN1539809 WB Mouse IgM, kappa Log in to see 340CT15-1-1
0.12118237 ABIN407052 ICC IF WB Rabbit C-Term, AA 350-436 Log in to see Polyclonal
0.12118237 ABIN407058 WB Rabbit AA 300-400, Internal Region Log in to see Polyclonal

Top referenced anti-PDPK1 antibody for Western Blotting

Similar anti-PDPK1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Pig (Porcine) Zebrafish (Danio rerio)
Western Blotting (WB) 140 Antibodies 68 Antibodies 67 Antibodies 6 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies 3 Antibodies
Immunoprecipitation (IP) 11 Antibodies 6 Antibodies 5 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 42 Antibodies 26 Antibodies 25 Antibodies
Immunohistochemistry (IHC) 55 Antibodies 37 Antibodies

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Epitope Middle Region
(49), (20), (19), (13), (13), (13), (13), (6), (6), (5), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio)
(248), (149), (147), (6), (6), (4), (4), (2), (1), (1), (1), (1)
Host Rabbit
(220), (30), (1)
Clonality
Polyclonal
Conjugate This PDPK1 antibody is un-conjugated
(9), (9), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (2), (1), (1)
Application Western Blotting (WB)
(132), (77), (65), (56), (42), (24), (12), (11), (5), (4), (3), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN2779320) Images
Blocking Peptide Blocking peptide for this product available: ABIN981552
Sequence IIHRDLKPEN ILLNEDMHIQ ITDFGTAKVL SPESKQARAN SFVGTAQYVS
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Rat : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against PDPK1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human PDPK1

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling References for anti-PDPK1 Antibody (ABIN2779320) Images back to top
Antigen
Alternative Name PDPK1 (PDPK1 Antibody Abstract)
Background PDPK1 phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. It may play a general role in signaling processes and in development.
Molecular Weight 63 kDa
Gene ID 5170
NCBI Accession NM_002613, NP_002604
UniProt O15530
Research Area Kinases/Phosphatases
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling References for anti-PDPK1 Antibody (ABIN2779320) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 556 AA

Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details References for anti-PDPK1 Antibody (ABIN2779320) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PDPK1 Antibody (ABIN2779320)

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Background publications

Lin, Hsieh, Song, Lin: "Elevated phosphorylation and activation of PDK-1/AKT pathway in human breast cancer." in: British journal of cancer, Vol. 93, Issue 12, pp. 1372-81, 2005

Images

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN2779320) back to top
Supplier Images
Western Blotting (WB) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (Middle Region) (ABIN2779320) WB Suggested Anti-PDPK1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Western Blotting (WB) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (Middle Region) (ABIN2779320) WB Suggested Anti-PDPK1
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500 ...