anti-Mouse (Murine) PDPK1 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • pdpk1
  • MGC82080
  • PDK1
  • PDPK2
  • PRO0461
  • Pdk1
  • zgc:153787
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • 3-phosphoinositide dependent protein kinase-1
  • 3-phosphoinositide dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase-1a
  • 3-phosphoinositide-dependent protein kinase 1
  • Phosphoinositide-dependent kinase 1
  • Protein PDK-1
  • PDPK1
  • pdpk1
  • Pdpk1
  • pdpk1a
  • Pdk1
  • pdk-1
Epitope
pSer241
58
35
32
27
27
26
18
17
15
15
15
15
7
7
6
6
6
5
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
464
220
220
19
13
12
11
11
6
5
4
3
2
1
1
1
1
1
1
1
Host
Rabbit
383
73
16
Clonality
Polyclonal
Conjugate
This PDPK1 antibody is un-conjugated
19
17
16
11
11
7
7
7
7
7
7
7
7
7
7
6
5
5
4
4
3
3
3
2
2
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
313
158
102
90
66
66
40
31
22
10
7
5
3
2
1
Options
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Catalog No. ABIN744668
$ 329.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN711116 IF (p) IHC (p) WB Rabbit IgG pTyr9 Log in to see Polyclonal
1 ABIN682948 IF (p) IHC (p) WB Rabbit IgG pTyr373, pTyr376 Log in to see Polyclonal
1 ABIN744673 IF (p) Cy5.5 Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN744672 IF (p) Cy5 Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN744674 IF (p) Cy7 Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN875009 IF (p) Alexa Fluor 555 Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN875011 IF (p) Alexa Fluor 488 Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN875013 IF (p) Alexa Fluor 350 Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN875012 IF (p) Alexa Fluor 647 Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN744671 IF (p) Cy3 Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN744675 IF (p) FITC Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN2814978 IF (p) Alexa Fluor 594 Rabbit IgG AA 220-260, pSer241 Log in to see Polyclonal
1 ABIN711134 IF (p) Cy3 Rabbit IgG pSer396 Log in to see Polyclonal
1 ABIN711029 IF (p) Cy3 Rabbit IgG pSer393 Log in to see Polyclonal
1 ABIN711030 IF (p) Cy5 Rabbit IgG pSer393 Log in to see Polyclonal
1 ABIN711033 IF (p) FITC Rabbit IgG pSer393 Log in to see Polyclonal
1 ABIN711120 IF (p) Cy5 Rabbit IgG pTyr9 Log in to see Polyclonal
1 ABIN711122 IF (p) Cy7 Rabbit IgG pTyr9 Log in to see Polyclonal
1 ABIN711149 IF (p) Cy3 Rabbit IgG pSer410 Log in to see Polyclonal
1 ABIN711153 IF (p) FITC Rabbit IgG pSer410 Log in to see Polyclonal

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Epitope pSer241
(58), (35), (32), (27), (27), (26), (18), (17), (15), (15), (15), (15), (7), (7), (6), (6), (6), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(464), (220), (220), (19), (13), (12), (11), (11), (6), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(383), (73), (16)
Clonality
Polyclonal
Conjugate This PDPK1 antibody is un-conjugated
(19), (17), (16), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(313), (158), (102), (90), (66), (66), (40), (31), (22), (10), (7), (5), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling References for anti-PDPK1 antibody (ABIN744668) Images
Specificity This phosphorylation site is homologous to Ser244 in Mouse and Rat.
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human PDK1 around the phosphorylation site of Ser241
Isotype IgG

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling References for anti-PDPK1 antibody (ABIN744668) Images back to top
Antigen
Alternative Name Pdk1 (PDPK1 Antibody Abstract)
Background

Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses.

Subcellular location: Cytoplasm, Membrane bound

Synonyms: PDK1, PDPK2, PDPK2P, PRO0461, 3-phosphoinositide-dependent protein kinase 1, hPDK1, PDPK1

Gene ID 5170
UniProt O15530
Research Area Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling References for anti-PDPK1 antibody (ABIN744668) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details References for anti-PDPK1 antibody (ABIN744668) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-PDPK1 antibody (ABIN744668)

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Product cited in:

Lin, Lin, Kang, Liu, Wang, Zheng, Yu, Lin: "Similar PDK1-AKT-mTOR pathway activation in balloon cells and dysmorphic neurons of type II focal cortical dysplasia with refractory epilepsy." in: Epilepsy research, Vol. 112, pp. 137-49, 2015

Images

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling References for anti-PDPK1 antibody (ABIN744668) back to top
Supplier Images
Western Blotting (WB) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer241) antibody (ABIN744668) Mouse bone lysates probed with Rabbit Anti-PDK1(ser241) Polyclonal Antibody, Unconjug...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer241) antibody (ABIN744668) paraformaldehyde-fixed, paraffin embedded human live carcinoma tissue, Antigen retrie...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer241) antibody (ABIN744668) paraformaldehyde-fixed, paraffin embedded human breast carcinoma tissue, Antigen retr...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer241) antibody (ABIN744668) Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boil...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer241) antibody (ABIN744668) Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boi...
Western Blotting (WB) image for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer241) antibody (ABIN744668) Lane 1: Mouse brain lysates, Lane 2: Mouse heart lysates probed with PDK1(Ser241) Pol...