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anti-Mouse (Murine) PDPK1 antibody for Western Blotting

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • pdpk1
  • MGC82080
  • zgc:153787
  • CD79B
  • LOC100304995
  • Pdk1
  • PDPK2
  • PRO0461
  • Phosphoinositide-dependent kinase 1
  • 3-phosphoinositide dependent protein kinase-1
  • 3-phosphoinositide dependent protein kinase-1a
  • CD79b molecule, immunoglobulin-associated beta
  • CD79b
  • 3-phosphoinositide-dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase 1
  • Protein PDK-1
  • Pdk1
  • pdpk1
  • pdpk1a
  • CD79B
  • CD79b
  • LOC100304995
  • Pdpk1
  • PDPK1
  • pdk-1
Epitope
N-Term
57
35
32
27
23
21
18
17
15
15
15
15
7
6
6
6
5
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
436
217
216
18
13
12
11
11
6
5
4
3
2
1
1
1
1
1
1
1
Host
Rabbit
359
68
16
Clonality
Polyclonal
Conjugate
This PDPK1 antibody is un-conjugated
17
17
14
11
11
7
7
7
7
7
7
7
7
7
6
5
5
5
4
4
3
3
3
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
288
152
93
91
60
59
38
27
20
10
6
5
3
2
1
Supplier
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Catalog No. ABIN359095
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0997953 ABIN1533064 ELISA WB Rabbit IgG Log in to see Polyclonal 1
3.0997953 ABIN967612 WB Mouse IgG1, kappa pSer241 Log in to see J66-653-44-22 2
3.0997953 ABIN744668 IF (p) IHC (p) WB Rabbit IgG pSer241 Log in to see Polyclonal 1
3.0997953 ABIN3043600 IHC (p) WB Rabbit IgG AA 524-556, C-Term Log in to see Polyclonal
3.0997953 ABIN968352 IF WB Mouse IgG1 AA 439-555 Log in to see 5-PDK1 5
3.0997953 ABIN968351 IF WB Mouse IgG1 AA 439-555 Log in to see 5-PDK1 5
3.0997953 ABIN1531949 IHC ELISA WB Rabbit IgG pSer241 Log in to see Polyclonal
3.0997953 ABIN3186375 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
3.0997953 ABIN3186376 ELISA IHC WB Rabbit IgG Thr222 Log in to see Polyclonal
3.0997953 ABIN223167 IP IHC WB Rabbit IgG Log in to see Polyclonal
3.0997953 ABIN1532981 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0997953 ABIN1870500 IF IHC WB Rabbit IgG pSer241 Log in to see Polyclonal
3.0997953 ABIN2706787 IP IHC WB Rabbit pSer241 Log in to see Polyclonal
3.0997953 ABIN1874088 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.0997953 ABIN2650941 IHC WB Rabbit IgG Log in to see Polyclonal
3.0997953 ABIN4344570 ICC IF IHC IHC (p) WB Rabbit pSer241 Log in to see Polyclonal
3.0997953 ABIN4344573 ICC IF IP WB Rabbit IgG Center Log in to see Polyclonal
3.0997953 ABIN2564451 ELISA WB Goat IgG Internal Region Log in to see Polyclonal
3.0997953 ABIN2404484 IF IHC WB Rabbit IgG Log in to see Polyclonal
3.0997953 ABIN1844665 IHC WB Rabbit pSer241 Log in to see Polyclonal

Top referenced anti-PDPK1 antibody for Western Blotting

Similar anti-PDPK1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 37 Antibodies 148 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 7 Antibodies
Flow Cytometry (FACS) 1 Antibodies 27 Antibodies
Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 19 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 91 Antibodies
Immunohistochemistry (IHC) 52 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 37 Antibodies
Immunoprecipitation (IP) 13 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 114 Antibodies
Dot Blot (DB) 10 Antibodies
ELISA (Capture) 2 Antibodies

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Epitope N-Term
(57), (35), (32), (27), (23), (21), (18), (17), (15), (15), (15), (15), (7), (6), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(436), (217), (216), (18), (13), (12), (11), (11), (6), (5), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(359), (68), (16)
Clonality
Polyclonal
Conjugate This PDPK1 antibody is un-conjugated
(17), (17), (14), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(288), (152), (93), (91), (60), (59), (38), (27), (20), (10), (6), (5), (3), (2), (1)
Pubmed 3 references available
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Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN359095) Images
Specificity This antibody reacts to PDK1.
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PDK1.
Isotype Ig

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling References for anti-PDPK1 Antibody (ABIN359095) Images back to top
Antigen
Alternative Name PDK1 (PDPK1 Antibody Abstract)
Background Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.Synonyms: Pyruvate dehydrogenase [lipoamide] kinase isozyme 1 mitochondrial, Pyruvate dehydrogenase kinase isoform 1
Gene ID 5163, 9606
UniProt Q15118
Research Area Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling References for anti-PDPK1 Antibody (ABIN359095) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details References for anti-PDPK1 Antibody (ABIN359095) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PDPK1 Antibody (ABIN359095)

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Background publications

Frödin, Antal, Dümmler, Jensen, Deak, Gammeltoft, Biondi: "A phosphoserine/threonine-binding pocket in AGC kinases and PDK1 mediates activation by hydrophobic motif phosphorylation." in: The EMBO journal, Vol. 21, Issue 20, pp. 5396-407, 2002

Sato, Fujita, Tsuruo: "Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39360-7, 2002

King, Zenke, Dawson, Dutil, Newton, Hemmings, Bokoch: "Sphingosine is a novel activator of 3-phosphoinositide-dependent kinase 1." in: The Journal of biological chemistry, Vol. 275, Issue 24, pp. 18108-13, 2000

Images

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN359095) back to top
Supplier Images
Western Blotting (WB) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (N-Term) (ABIN359095) The anti-PDK1 Pab is used in Western blot to detect PDK1 in mouse kidney tissue lysate.