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anti-Rat (Rattus) PDPK1 antibody for Intracellular Staining

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • 1210
  • BG02759
  • CD79B
  • CG1201
  • CG1210
  • Dmel\\CG1210
  • dPDK-1
  • dPDK1
  • Dstpk61
  • DSTPK61
  • dSTPK61
  • LOC100304995
  • MGC82080
  • PDK
  • PDK-1
  • PDK1
  • Pdk1
  • PDK1/Pk61C
  • pdpk1
  • PDPK2
  • Pk61C
  • pk61c
  • PK61C
  • Pk61C/PDK1
  • PRO0461
  • zgc:153787
Epitope
pSer241
49
20
19
19
13
13
13
13
13
11
8
8
6
6
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
289
151
149
7
6
4
4
2
1
1
1
1
1
1
1
Host
Mouse
243
48
1
Clonality
Monoclonal
Conjugate
This PDPK1 antibody is conjugated to PE
12
11
11
10
9
7
7
7
7
7
7
7
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
Application
Intracellular Staining (ICS)
173
85
77
61
51
46
32
19
12
11
4
2
1
1
1
Supplier
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Catalog No. ABIN1177136
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008696448 ABIN1177137 ICS Alexa Fluor 647 Mouse IgG1, kappa pSer241 Log in to see J66-653-44-17 1
0.0008696448 ABIN1177138 ICS Alexa Fluor 488 Mouse IgG1, kappa pSer241 Log in to see J66-653-44-17 1

Top referenced anti-PDPK1 antibody for Intracellular Staining

Similar anti-PDPK1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 180 Antibodies 70 Antibodies 69 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies 3 Antibodies
Immunoprecipitation (IP) 11 Antibodies 6 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 46 Antibodies 27 Antibodies 27 Antibodies
Immunohistochemistry (IHC) 60 Antibodies 38 Antibodies 38 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 77 Antibodies

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Epitope pSer241
(49), (20), (19), (19), (13), (13), (13), (13), (13), (11), (8), (8), (6), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(289), (151), (149), (7), (6), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(243), (48), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PDPK1 antibody is conjugated to PE
(12), (11), (11), (10), (9), (7), (7), (7), (7), (7), (7), (7), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS)
(173), (85), (77), (61), (51), (46), (32), (19), (12), (11), (4), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN1177136) Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human PDPK1 Peptide
Clone J66-653-44-17
Isotype IgG1, kappa

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling References for anti-PDPK1 Antibody (ABIN1177136) Images back to top
Antigen
Alternative Name PDPK1 (PDPK1 Antibody Abstract)
Background The serine/threonine kinase 3-Phosphoinositide-Dependent Protein Kinase-1 (PDPK1, also known as PDK1) contributes to the activation of many important kinases in the insulin and IGF-1 signaling pathways. It acts downstream of phosphatidylinositol 3-kinase (PI3-kinase) to phosphorylate residues in the activation loops of many cellular kinases, including protein kinase B (PKB/Akt), PKC isoforms, p70 S6 kinase, and PDPK1 itself. The autophosphorylation of PDPK1 at serine 241 (S241) has recently been suggested to play a role in the regulation of PDPK1. It has been proposed that PDPK1 activity plays a key role in the regulation of various cellular events such as cell proliferation, differentiation, and apoptosis. The J666-653.44.17 monoclonal antibody recognizes the phosphorylated S241 in the activation loop of human PDPK1. The orthologous phosphorylation site in mouse and rat PDPK1 is S244.
Synonyms: PDK1 (pS241), PKB Kinase (pS241)
Research Area Kinases/Phosphatases
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling References for anti-PDPK1 Antibody (ABIN1177136) Images back to top
Sample Volume 20 μL
Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details References for anti-PDPK1 Antibody (ABIN1177136) Images back to top
Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References for anti-PDPK1 Antibody (ABIN1177136)

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Product cited in:

Komander, Kular, Deak, Alessi, van Aalten: "Role of T-loop phosphorylation in PDK1 activation, stability, and substrate binding." in: The Journal of biological chemistry, Vol. 280, Issue 19, pp. 18797-802, 2005