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anti-Rat (Rattus) PDPK1 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • pdpk1
  • MGC82080
  • zgc:153787
  • CD79B
  • LOC100304995
  • Pdk1
  • PDPK2
  • PRO0461
  • Phosphoinositide-dependent kinase 1
  • 3-phosphoinositide dependent protein kinase-1
  • 3-phosphoinositide dependent protein kinase-1a
  • CD79b molecule, immunoglobulin-associated beta
  • CD79b
  • 3-phosphoinositide-dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase 1
  • Protein PDK-1
  • Pdk1
  • pdpk1
  • pdpk1a
  • CD79B
  • CD79b
  • LOC100304995
  • Pdpk1
  • PDPK1
  • pdk-1
Epitope
pSer241
57
35
32
26
24
21
18
17
15
15
15
15
9
6
6
6
5
5
5
4
4
4
4
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
447
224
224
19
15
11
11
10
6
3
3
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
366
71
17
Clonality
Polyclonal
Conjugate
This PDPK1 antibody is un-conjugated
17
17
14
11
11
7
7
7
7
7
7
7
7
7
6
5
5
5
4
4
3
3
3
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
299
157
99
90
60
58
38
27
20
11
7
5
3
2
1
Options
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Catalog No. ABIN744668
$ 329.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN682948 IF (p) IHC (p) WB Rabbit IgG pTyr373, pTyr376 Log in to see Polyclonal
1 ABIN711116 IF (p) IHC (p) WB Rabbit IgG pTyr9 Log in to see Polyclonal
1 ABIN2814978 IF (p) Alexa Fluor 594 Rabbit IgG AA 220-260, pSer241 Log in to see Polyclonal
1 ABIN744675 IF (p) FITC Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN744671 IF (p) Cy3 Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN744673 IF (p) Cy5.5 Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN744674 IF (p) Cy7 Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN875011 IF (p) Alexa Fluor 488 Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN875009 IF (p) Alexa Fluor 555 Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN875012 IF (p) Alexa Fluor 647 Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN875013 IF (p) Alexa Fluor 350 Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN744672 IF (p) Cy5 Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN880352 IF (p) Alexa Fluor 350 Rabbit IgG pTyr9 Log in to see Polyclonal
1 ABIN880354 IF (p) Alexa Fluor 647 Rabbit IgG pTyr9 Log in to see Polyclonal
1 ABIN2805224 IF (p) Alexa Fluor 594 Rabbit IgG pSer396 Log in to see Polyclonal
1 ABIN2805225 IF (p) Alexa Fluor 594 Rabbit IgG pSer410 Log in to see Polyclonal
1 ABIN2805221 IF (p) Alexa Fluor 594 Rabbit IgG pTyr373, pTyr376 Log in to see Polyclonal
1 ABIN2805222 IF (p) Alexa Fluor 594 Rabbit IgG pSer393 Log in to see Polyclonal
1 ABIN2805223 IF (p) Alexa Fluor 594 Rabbit IgG pTyr9 Log in to see Polyclonal
1 ABIN682951 IF (p) Cy3 Rabbit IgG pTyr373, pTyr376 Log in to see Polyclonal

Top referenced anti-PDPK1 antibody for Immunofluorescence (Paraffin-embedded Sections)

Similar anti-PDPK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 44 Antibodies 41 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 1 Antibodies
Flow Cytometry (FACS) 8 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 3 Antibodies
Immunofluorescence (IF) 21 Antibodies 19 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies 3 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 91 Antibodies 91 Antibodies
Immunohistochemistry (IHC) 60 Antibodies 57 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 40 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 119 Antibodies

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Epitope pSer241
(57), (35), (32), (26), (24), (21), (18), (17), (15), (15), (15), (15), (9), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(447), (224), (224), (19), (15), (11), (11), (10), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(366), (71), (17)
Clonality
Polyclonal
Conjugate This PDPK1 antibody is un-conjugated
(17), (17), (14), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(299), (157), (99), (90), (60), (58), (38), (27), (20), (11), (7), (5), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN744668) Images
Specificity This phosphorylation site is homologous to Ser244 in Mouse and Rat.
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human PDK1 around the phosphorylation site of Ser241
Isotype IgG

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling References for anti-PDPK1 Antibody (ABIN744668) Images back to top
Antigen
Alternative Name Pdk1 (PDPK1 Antibody Abstract)
Background

Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses.

Subcellular location: Cytoplasm, Membrane bound


Synonyms: PDK1, PDPK2, PDPK2P, PRO0461, 3-phosphoinositide-dependent protein kinase 1, hPDK1, PDPK1
Gene ID 5170
UniProt O15530
Research Area Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling References for anti-PDPK1 Antibody (ABIN744668) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details References for anti-PDPK1 Antibody (ABIN744668) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-PDPK1 Antibody (ABIN744668)

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Product cited in:

Lin, Lin, Kang, Liu, Wang, Zheng, Yu, Lin: "Similar PDK1-AKT-mTOR pathway activation in balloon cells and dysmorphic neurons of type II focal cortical dysplasia with refractory epilepsy." in: Epilepsy research, Vol. 112, pp. 137-49, 2015

Images

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN744668) back to top
Supplier Images
Western Blotting (WB) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pSer241) (ABIN744668) Mouse bone lysates probed with Rabbit Anti-PDK1(ser241) Polyclonal Antibody, Unconjug...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pSer241) (ABIN744668) paraformaldehyde-fixed, paraffin embedded human live carcinoma tissue, Antigen retrie...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pSer241) (ABIN744668) paraformaldehyde-fixed, paraffin embedded human breast carcinoma tissue, Antigen retr...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pSer241) (ABIN744668) Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boil...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pSer241) (ABIN744668) Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boi...
Western Blotting (WB) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pSer241) (ABIN744668) Lane 1: Mouse brain lysates, Lane 2: Mouse heart lysates probed with PDK1(Ser241) Pol...