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anti-Rat (Rattus) PDPK1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • 1210
  • BG02759
  • CD79B
  • CG1201
  • CG1210
  • Dmel\\CG1210
  • dPDK-1
  • dPDK1
  • Dstpk61
  • DSTPK61
  • dSTPK61
  • LOC100304995
  • MGC82080
  • PDK
  • PDK-1
  • PDK1
  • Pdk1
  • PDK1/Pk61C
  • pdpk1
  • PDPK2
  • Pk61C
  • pk61c
  • PK61C
  • Pk61C/PDK1
  • PRO0461
  • zgc:153787
Epitope
pSer241
49
20
19
19
13
13
13
13
13
11
8
8
6
6
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
289
151
149
7
6
4
4
2
1
1
1
1
1
1
1
Host
Rabbit
242
49
1
Clonality
Polyclonal
Conjugate
This PDPK1 antibody is un-conjugated
12
11
11
10
9
7
7
7
7
7
7
7
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
172
85
77
61
50
45
32
19
12
11
4
3
1
1
1
Supplier
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Catalog No. ABIN196616
$ 357.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008982679 ABIN127128 IHC (p) IP WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN3043600 IHC (p) WB Rabbit IgG AA 524-556, C-Term Log in to see Polyclonal
0.0008982679 ABIN744668 IF (p) IHC (p) WB Rabbit IgG pSer241 Log in to see Polyclonal 1
0.0008982679 ABIN711116 IF (p) IHC (p) WB Rabbit IgG pTyr9 Log in to see Polyclonal
0.0008982679 ABIN682948 IF (p) IHC (p) WB Rabbit IgG pTyr373, pTyr376 Log in to see Polyclonal
0.0008982679 ABIN1737955 IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0008982679 ABIN711028 IHC (p) WB Biotin Rabbit IgG pSer393 Log in to see Polyclonal
0.0008982679 ABIN711035 IHC (p) WB HRP Rabbit IgG pSer393 Log in to see Polyclonal
0.0008982679 ABIN711118 IHC (p) WB Biotin Rabbit IgG pTyr9 Log in to see Polyclonal
0.0008982679 ABIN711133 IHC (p) WB Biotin Rabbit IgG pSer396 Log in to see Polyclonal
0.0008982679 ABIN711125 IHC (p) WB HRP Rabbit IgG pTyr9 Log in to see Polyclonal
0.0008982679 ABIN711140 IHC (p) WB HRP Rabbit IgG pSer396 Log in to see Polyclonal
0.0008982679 ABIN711148 IHC (p) WB Biotin Rabbit IgG pSer410 Log in to see Polyclonal
0.0008982679 ABIN711155 IHC (p) WB HRP Rabbit IgG pSer410 Log in to see Polyclonal
0.0008982679 ABIN682950 IHC (p) WB Biotin Rabbit IgG pTyr373, pTyr376 Log in to see Polyclonal
0.0008982679 ABIN682957 IHC (p) WB HRP Rabbit IgG pTyr373, pTyr376 Log in to see Polyclonal
0.0008982679 ABIN744677 IHC (p) WB HRP Rabbit IgG pSer241 Log in to see Polyclonal
0.0008982679 ABIN744670 IHC (p) WB Biotin Rabbit IgG pSer241 Log in to see Polyclonal
0.0008982679 ABIN711026 IF (p) IHC (p) WB Rabbit IgG pSer393 Log in to see Polyclonal
0.0008982679 ABIN711131 IF (p) IHC (p) WB Rabbit IgG pSer396 Log in to see Polyclonal

Top referenced anti-PDPK1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-PDPK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 18 Antibodies 16 Antibodies
Immunocytochemistry (ICC) 6 Antibodies 6 Antibodies
Immunofluorescence (IF) 16 Antibodies 17 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 77 Antibodies 77 Antibodies
Immunohistochemistry (IHC) 38 Antibodies 38 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies 27 Antibodies
Immunoprecipitation (IP) 5 Antibodies 6 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies
Western Blotting (WB) 69 Antibodies 70 Antibodies

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Epitope pSer241
(49), (20), (19), (19), (13), (13), (13), (13), (13), (11), (8), (8), (6), (6), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(289), (151), (149), (7), (6), (4), (4), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(242), (49), (1)
Clonality
Polyclonal
Conjugate This PDPK1 antibody is un-conjugated
(12), (11), (11), (10), (9), (7), (7), (7), (7), (7), (7), (7), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(172), (85), (77), (61), (50), (45), (32), (19), (12), (11), (4), (3), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN196616) Images
Specificity The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. PDK1 (phospho-Ser241) antibody detects endogenous levels of PDK1 only when phosphorylated at serine 241.
Purification Immunoaffinity chromatography.
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human PDK1 around the phosphorylation site of serine 241 (A-N-SP-F-V).

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling References for anti-PDPK1 Antibody (ABIN196616) Images back to top
Antigen
Alternative Name PDPK1 (PDPK1 Antibody Abstract)
Background Synonyms: 3-phosphoinositide-dependent proteinkinase 1, PDK-1, PDK1, PDPK1, hPDK1
Gene ID 5170
NCBI Accession NP_002604
UniProt O15530
Research Area Kinases/Phosphatases
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling References for anti-PDPK1 Antibody (ABIN196616) Images back to top
Application Notes Suitable for use in Western blot (1: 500approx. 1: 1000) and Immunohistochemistry (1: 50approx. 1: 100).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details References for anti-PDPK1 Antibody (ABIN196616) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.

References for anti-PDPK1 Antibody (ABIN196616)

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Background publications

Scheid, Parsons, Woodgett: "Phosphoinositide-dependent phosphorylation of PDK1 regulates nuclear translocation." in: Molecular and cellular biology, Vol. 25, Issue 6, pp. 2347-63, 2005

Lim, Kikani, Wick, Dong: "Nuclear translocation of 3'-phosphoinositide-dependent protein kinase 1 (PDK-1): a potential regulatory mechanism for PDK-1 function." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 24, pp. 14006-11, 2003

Sato, Fujita, Tsuruo: "Regulation of kinase activity of 3-phosphoinositide-dependent protein kinase-1 by binding to 14-3-3." in: The Journal of biological chemistry, Vol. 277, Issue 42, pp. 39360-7, 2002

Chen, Nystrom, Dong, Li, Song, Liu, Quon: "Insulin stimulates increased catalytic activity of phosphoinositide-dependent kinase-1 by a phosphorylation-dependent mechanism." in: Biochemistry, Vol. 40, Issue 39, pp. 11851-9, 2001

Images

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN196616) back to top
Supplier Images
 image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pSer241) (ABIN196616) anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer241) antibody
 image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pSer241) (ABIN196616) anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer241) antibody (Image 2)
 image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pSer241) (ABIN196616) anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (pSer241) antibody (Image 3)