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anti-Rat (Rattus) PDPK1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • pdpk1
  • MGC82080
  • zgc:153787
  • CD79B
  • LOC100304995
  • Pdk1
  • PDPK2
  • PRO0461
  • Phosphoinositide-dependent kinase 1
  • 3-phosphoinositide dependent protein kinase-1
  • 3-phosphoinositide dependent protein kinase-1a
  • CD79b molecule, immunoglobulin-associated beta
  • CD79b
  • 3-phosphoinositide-dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase 1
  • Protein PDK-1
  • Pdk1
  • pdpk1
  • pdpk1a
  • CD79B
  • CD79b
  • LOC100304995
  • Pdpk1
  • PDPK1
  • pdk-1
Epitope
pSer241
57
38
32
25
24
21
18
17
15
15
15
15
10
6
6
6
5
5
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
444
198
192
10
10
8
7
6
4
1
1
1
1
1
1
1
1
Host
Rabbit
364
71
17
Clonality
Polyclonal
Conjugate
This PDPK1 antibody is un-conjugated
17
17
14
11
11
7
7
7
7
7
7
7
7
7
6
5
5
5
4
4
3
3
3
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
290
153
101
90
62
53
40
27
19
11
7
3
2
1
Options
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Catalog No. ABIN744668
$ 329.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.082888 ABIN4344570 ICC IF IHC IHC (p) WB Rabbit pSer241 Log in to see Polyclonal
12.082888 ABIN4344574 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4A11
1 ABIN196616 IF IHC (p) WB Rabbit pSer241 Log in to see Polyclonal 4
1 ABIN3043600 IHC (p) WB Rabbit IgG AA 524-556, C-Term Log in to see Polyclonal
1 ABIN127128 IHC (p) IP WB Rabbit Log in to see Polyclonal
1 ABIN3044043 IHC (p) WB Rabbit IgG AA 408-421, C-Term Log in to see Polyclonal
1 ABIN1108608 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 4A11 3
1 ABIN1737955 IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN682948 IF (p) IHC (p) WB Rabbit IgG pTyr373, pTyr376 Log in to see Polyclonal
1 ABIN711116 IF (p) IHC (p) WB Rabbit IgG pTyr9 Log in to see Polyclonal
1 ABIN744677 IHC (p) WB HRP Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN744670 IHC (p) WB Biotin Rabbit IgG pSer241 Log in to see Polyclonal
1 ABIN682957 IHC (p) WB HRP Rabbit IgG pTyr373, pTyr376 Log in to see Polyclonal
1 ABIN711125 IHC (p) WB HRP Rabbit IgG pTyr9 Log in to see Polyclonal
1 ABIN711035 IHC (p) WB HRP Rabbit IgG pSer393 Log in to see Polyclonal
1 ABIN711155 IHC (p) WB HRP Rabbit IgG pSer410 Log in to see Polyclonal
1 ABIN711140 IHC (p) WB HRP Rabbit IgG pSer396 Log in to see Polyclonal
1 ABIN682950 IHC (p) WB Biotin Rabbit IgG pTyr373, pTyr376 Log in to see Polyclonal
1 ABIN711133 IHC (p) WB Biotin Rabbit IgG pSer396 Log in to see Polyclonal
1 ABIN711028 IHC (p) WB Biotin Rabbit IgG pSer393 Log in to see Polyclonal

Top referenced anti-PDPK1 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-PDPK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 36 Antibodies 29 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 1 Antibodies
Flow Cytometry (FACS) 8 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 9 Antibodies 6 Antibodies
Immunofluorescence (IF) 21 Antibodies 18 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 91 Antibodies 91 Antibodies
Immunohistochemistry (IHC) 54 Antibodies 51 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 30 Antibodies 27 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 95 Antibodies

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Epitope pSer241
(57), (38), (32), (25), (24), (21), (18), (17), (15), (15), (15), (15), (10), (6), (6), (6), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(444), (198), (192), (10), (10), (8), (7), (6), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(364), (71), (17)
Clonality
Polyclonal
Conjugate This PDPK1 antibody is un-conjugated
(17), (17), (14), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(290), (153), (101), (90), (62), (53), (40), (27), (19), (11), (7), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN744668) Images
Specificity This phosphorylation site is homologous to Ser244 in Mouse and Rat.
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human PDK1 around the phosphorylation site of Ser241
Isotype IgG

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling References for anti-PDPK1 Antibody (ABIN744668) Images back to top
Antigen
Alternative Name Pdk1 (PDPK1 Antibody Abstract)
Background

Serine/threonine kinase which acts as a master kinase, phosphorylating and activating a subgroup of the AGC family of protein kinases. Its targets include: protein kinase B (PKB/AKT1, PKB/AKT2, PKB/AKT3), p70 ribosomal protein S6 kinase (RPS6KB1), p90 ribosomal protein S6 kinase (RPS6KA1, RPS6KA2 and RPS6KA3), cyclic AMP-dependent protein kinase (PRKACA), protein kinase C (PRKCD and PRKCZ), serum and glucocorticoid-inducible kinase (SGK1, SGK2 and SGK3), p21-activated kinase-1 (PAK1), protein kinase PKN (PKN1 and PKN2). Plays a central role in the transduction of signals from insulin by providing the activating phosphorylation to PKB/AKT1, thus propagating the signal to downstream targets controlling cell proliferation and survival, as well as glucose and amino acid uptake and storage. Negatively regulates the TGF-beta-induced signaling by: modulating the association of SMAD3 and SMAD7 with TGF-beta receptor, phosphorylating SMAD2, SMAD3, SMAD4 and SMAD7, preventing the nuclear translocation of SMAD3 and SMAD4 and the translocation of SMAD7 from the nucleus to the cytoplasm in response to TGF-beta. Activates PPARG transcriptional activity and promotes adipocyte differentiation. Activates the NF-kappa-B pathway via phosphorylation of IKKB. The tyrosine phosphorylated form is crucial for the regulation of focal adhesions by angiotensin II. Controls proliferation, survival, and growth of developing pancreatic cells. Participates in the regulation of Ca(2+) entry and Ca(2+)-activated K(+) channels of mast cells. Essential for the motility of vascular endothelial cells (ECs) and is involved in the regulation of their chemotaxis. Plays a critical role in cardiac homeostasis by serving as a dual effector for cell survival and beta-adrenergic response. Plays an important role during thymocyte development by regulating the expression of key nutrient receptors on the surface of pre-T cells and mediating Notch-induced cell growth and proliferative responses.

Subcellular location: Cytoplasm, Membrane bound


Synonyms: PDK1, PDPK2, PDPK2P, PRO0461, 3-phosphoinositide-dependent protein kinase 1, hPDK1, PDPK1
Gene ID 5170
UniProt O15530
Research Area Signaling, Cytoskeleton, Microfilaments, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling References for anti-PDPK1 Antibody (ABIN744668) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details References for anti-PDPK1 Antibody (ABIN744668) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-PDPK1 Antibody (ABIN744668)

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Product cited in:

Lin, Lin, Kang, Liu, Wang, Zheng, Yu, Lin: "Similar PDK1-AKT-mTOR pathway activation in balloon cells and dysmorphic neurons of type II focal cortical dysplasia with refractory epilepsy." in: Epilepsy research, Vol. 112, pp. 137-49, 2015

Images

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN744668) back to top
Supplier Images
Western Blotting (WB) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pSer241) (ABIN744668) Mouse bone lysates probed with Rabbit Anti-PDK1(ser241) Polyclonal Antibody, Unconjug...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pSer241) (ABIN744668) paraformaldehyde-fixed, paraffin embedded human live carcinoma tissue, Antigen retrie...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pSer241) (ABIN744668) paraformaldehyde-fixed, paraffin embedded human breast carcinoma tissue, Antigen retr...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pSer241) (ABIN744668) Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boil...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pSer241) (ABIN744668) Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boi...
Western Blotting (WB) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (pSer241) (ABIN744668) Lane 1: Mouse brain lysates, Lane 2: Mouse heart lysates probed with PDK1(Ser241) Pol...