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anti-Rat (Rattus) PDPK1 antibody for Western Blotting

Recommended PDPK1 Antibody (supplied by: Log in to see )

Antigen
3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
  • 1210
  • BG02759
  • CG1201
  • CG1210
  • DSTPK61
  • Dmel\\CG1210
  • Dstpk61
  • PDK
  • PDK-1
  • PDK1
  • PDK1/Pk61C
  • PK61C
  • Pk61C
  • Pk61C/PDK1
  • dPDK-1
  • dPDK1
  • dSTPK61
  • pk61c
  • pdpk1
  • MGC82080
  • zgc:153787
  • CD79B
  • LOC100304995
  • Pdk1
  • PDPK2
  • PRO0461
  • Phosphoinositide-dependent kinase 1
  • 3-phosphoinositide dependent protein kinase-1
  • 3-phosphoinositide dependent protein kinase-1a
  • CD79b molecule, immunoglobulin-associated beta
  • CD79b
  • 3-phosphoinositide-dependent protein kinase 1
  • 3-phosphoinositide dependent protein kinase 1
  • Protein PDK-1
  • Pdk1
  • pdpk1
  • pdpk1a
  • CD79B
  • CD79b
  • LOC100304995
  • Pdpk1
  • PDPK1
  • pdk-1
Epitope
Middle Region
58
35
32
26
24
21
18
17
15
15
15
15
9
6
6
6
5
5
5
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio)
447
224
224
19
14
11
11
10
6
3
3
2
2
1
1
1
1
1
1
1
Host
Rabbit
366
71
17
Clonality
Polyclonal
Conjugate
This PDPK1 antibody is un-conjugated
17
17
14
11
11
7
7
7
7
7
7
7
7
7
6
5
5
5
4
4
3
3
3
1
1
1
1
1
1
Application
Western Blotting (WB)
299
157
99
91
60
59
38
27
20
11
7
5
3
2
1
Options
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Catalog No. ABIN2779320
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9590726 ABIN2777432 WB Rabbit Middle Region Log in to see Polyclonal 1
2.9590726 ABIN1533064 ELISA WB Rabbit IgG Log in to see Polyclonal 1
2.9590726 ABIN967612 WB Mouse IgG1, kappa pSer241 Log in to see J66-653-44-22 2
2.9590726 ABIN744668 IF (p) IHC (p) WB Rabbit IgG pSer241 Log in to see Polyclonal 1
2.9590726 ABIN968352 IF WB Mouse IgG1 AA 439-555 Log in to see 5-PDK1 5
2.9590726 ABIN968351 IF WB Mouse IgG1 AA 439-555 Log in to see 5-PDK1 5
2.9590726 ABIN969347 ICC FACS IHC ELISA WB Mouse IgG1 Log in to see 4A11 3
2.9590726 ABIN3043600 IHC (p) WB Rabbit IgG AA 524-556, C-Term Log in to see Polyclonal
2.9590726 ABIN269845 ELISA WB Goat Log in to see Polyclonal 1
2.9590726 ABIN1531949 IHC ELISA WB Rabbit IgG pSer241 Log in to see Polyclonal
2.9590726 ABIN400866 EIA WB Mouse IgG1 AA 29-436 Log in to see AT2D6
2.9590726 ABIN3186375 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
2.9590726 ABIN3186376 ELISA IHC WB Rabbit IgG Thr222 Log in to see Polyclonal
2.9590726 ABIN3044043 IHC (p) WB Rabbit IgG AA 408-421, C-Term Log in to see Polyclonal
2.9590726 ABIN223167 IP IHC WB Rabbit IgG Log in to see Polyclonal
2.9590726 ABIN1532981 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
2.9590726 ABIN1870500 IF IHC WB Rabbit IgG pSer241 Log in to see Polyclonal
2.9590726 ABIN2706787 IP IHC WB Rabbit pSer241 Log in to see Polyclonal
2.9590726 ABIN1108608 EIA FACS IF IHC (p) WB Mouse IgG1 Log in to see 4A11 3
2.9590726 ABIN2499647 IHC WB Rabbit IgG Ser241 Log in to see Polyclonal

Top referenced anti-PDPK1 antibody for Western Blotting

Similar anti-PDPK1 Antibodies

Application / Reactivity Human Rat (Rattus) Mouse (Murine) Pig (Porcine) Zebrafish (Danio rerio)
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies 4 Antibodies 3 Antibodies
Western Blotting (WB) 293 Antibodies 121 Antibodies 119 Antibodies 14 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Intracellular Staining (ICS) 3 Antibodies 3 Antibodies 3 Antibodies
Immunoprecipitation (IP) 19 Antibodies 13 Antibodies 10 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 59 Antibodies 37 Antibodies 40 Antibodies

General

Antigen 3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) Antibodies
Epitope Middle Region
(58), (35), (32), (26), (24), (21), (18), (17), (15), (15), (15), (15), (9), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Zebrafish (Danio rerio)
(447), (224), (224), (19), (14), (11), (11), (10), (6), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(366), (71), (17)
Clonality
Polyclonal
Conjugate This PDPK1 antibody is un-conjugated
(17), (17), (14), (11), (11), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(299), (157), (99), (91), (60), (59), (38), (27), (20), (11), (7), (5), (3), (2), (1)
Pubmed 1 reference available
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Product Details anti-PDPK1 Antibody

Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN2779320) Images
Blocking Peptide Blocking peptide for this product available: ABIN981552
Sequence IIHRDLKPEN ILLNEDMHIQ ITDFGTAKVL SPESKQARAN SFVGTAQYVS
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Guinea pig : 100 %, Human : 100 %, Mouse : 100 %, Rat : 100 %, Zebrafish : 100 %
Characteristics This is a rabbit polyclonal antibody against PDPK1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human PDPK1

Target Details PDPK1

Product Details anti-PDPK1 Antibody Application Details Handling References for anti-PDPK1 Antibody (ABIN2779320) Images back to top
Antigen
Alternative Name PDPK1 (PDPK1 Antibody Abstract)
Background PDPK1 phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. It may play a general role in signaling processes and in development.
Molecular Weight 63 kDa
Gene ID 5170
NCBI Accession NM_002613, NP_002604
UniProt O15530
Research Area Kinases/Phosphatases
Pathways PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity

Application Details

Product Details anti-PDPK1 Antibody Target Details PDPK1 Handling References for anti-PDPK1 Antibody (ABIN2779320) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 556 AA

Restrictions For Research Use only

Handling

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details References for anti-PDPK1 Antibody (ABIN2779320) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-PDPK1 Antibody (ABIN2779320)

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling Images back to top
Background publications

Lin, Hsieh, Song, Lin: "Elevated phosphorylation and activation of PDK-1/AKT pathway in human breast cancer." in: British journal of cancer, Vol. 93, Issue 12, pp. 1372-81, 2005

Images

Product Details anti-PDPK1 Antibody Target Details PDPK1 Application Details Handling References for anti-PDPK1 Antibody (ABIN2779320) back to top
Supplier Images
Western Blotting (WB) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (Middle Region) (ABIN2779320) WB Suggested Anti-PDPK1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Western Blotting (WB) image for anti-PDPK1 antibody (3-phosphoinositide Dependent Protein Kinase-1) (Middle Region) (ABIN2779320) WB Suggested Anti-PDPK1
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:.12500 ...