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anti-Human PHLPP1 antibody for Western Blotting

Recommended PHLPP1 Antibody (supplied by: Log in to see )

Antigen
PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1) Antibodies
  • AI836256
  • mKIAA0606
  • PHLPP
  • Phlpp
  • PLEKHE1
  • Plekhe1
  • Scop
  • SCOP
Epitope
N-Term
12
7
3
2
1
1
1
1
1
1
Reactivity
Human
32
7
5
Host
Rabbit
32
Clonality
Polyclonal
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
26
10
6
5
4
3
2
1
1
Supplier
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Catalog No. ABIN1449987
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN392310 WB Rabbit Ig AA 531-560, N-Term Log in to see Polyclonal
0.12303223 ABIN2705120 WB Rabbit C-Term Log in to see Polyclonal
0.12303223 ABIN151320 ICC IP WB Rabbit C-Term Log in to see Polyclonal
0.12303223 ABIN1031510 ELISA WB Rabbit N-Term Log in to see Polyclonal
0.12303223 ABIN2492557 ELISA WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.0011806369 ABIN360194 EIA WB Rabbit Ig N-Term Log in to see Polyclonal 3
0.0011806369 ABIN1804212 IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.0011806369 ABIN481040 IP WB Rabbit C-Term Log in to see Polyclonal
0.0011806369 ABIN603650 ICC WB Rabbit C-Term Log in to see Polyclonal
0.0011806369 ABIN1453893 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
0.0011806369 ABIN2884713 ELISA WB Rabbit IgG AA 1194-1243 Log in to see Polyclonal
0.0011806369 ABIN2334564 ELISA WB Rabbit IgG N-Term, PH domain Log in to see Polyclonal
0.0011806369 ABIN2580264 WB Rabbit C-Term Log in to see Polyclonal
0.0011806369 ABIN2451738 ICC IHC (p) WB Rabbit IgG AA 1192-1205, AA 1704-1717 Log in to see Polyclonal
0.0011806369 ABIN1933140 IP WB Rabbit IgG C-Term Log in to see Polyclonal
0.0011806369 ABIN2334563 IC WB Rabbit C-Term, PH domain Log in to see Polyclonal
0.0011806369 ABIN1840456 WB Rabbit N-Term Log in to see Polyclonal
0.0011806369 ABIN2878332 WB Rabbit Log in to see Polyclonal
0.0011806369 ABIN1888364 ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0011806369 ABIN1933139 IP WB Rabbit AA 1175-1225 Log in to see Polyclonal

Top referenced anti-PHLPP1 antibody for Western Blotting

Similar anti-PHLPP1 Antibodies

Application / Reactivity Human
ELISA 10 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunohistochemistry (IHC) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 5 Antibodies
Immunoprecipitation (IP) 8 Antibodies
Western Blotting (WB) 28 Antibodies

General

Antigen PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1) Antibodies
Epitope N-Term
(12), (7), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(32), (7), (5)
Host Rabbit
(32)
Clonality
Polyclonal
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(26), (10), (6), (5), (4), (3), (2), (1), (1)
Pubmed 4 references available
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Product Details anti-PHLPP1 Antibody

Target Details PHLPP1 Application Details Handling References for anti-PHLPP1 Antibody (ABIN1449987) Images
Purification Affinity chromatography purified via peptide column
Immunogen PHLPP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of Human PHLPP1
Isotype IgG

Target Details PHLPP1

Product Details anti-PHLPP1 Antibody Application Details Handling References for anti-PHLPP1 Antibody (ABIN1449987) Images back to top
Antigen
Alternative Name PHLPP1 (PHLPP1 Antibody Abstract)
Background PHLPP1 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. Increased expression of PHLPP1 may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.Synonyms: KIAA0606, PH domain leucine-rich repeat-containing protein phosphatase 1, PHLPP, PLEKHE1, Pleckstrin homology domain-containing family E member 1, SCOP, Suprachiasmatic nucleus circadian oscillatory protein
Molecular Weight 189 kDa
Gene ID 23239
NCBI Accession NP_919431
Pathways PI3K-Akt Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PHLPP1 Antibody Target Details PHLPP1 Handling References for anti-PHLPP1 Antibody (ABIN1449987) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-PHLPP1 Antibody Target Details PHLPP1 Application Details References for anti-PHLPP1 Antibody (ABIN1449987) Images back to top
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-PHLPP1 Antibody (ABIN1449987)

Product Details anti-PHLPP1 Antibody Target Details PHLPP1 Application Details Handling Images back to top
Background publications

Andreozzi, Procopio, Greco, Mannino, Miele, Raciti, Iadicicco, Beguinot, Pontiroli, Hribal, Folli, Sesti: "Increased levels of the Akt-specific phosphatase PH domain leucine-rich repeat protein phosphatase (PHLPP)-1 in obese participants are associated with insulin resistance." in: Diabetologia, Vol. 54, Issue 7, pp. 1879-87, 2011

Brognard, Newton: "PHLiPPing the switch on Akt and protein kinase C signaling." in: Trends in endocrinology and metabolism: TEM, Vol. 19, Issue 6, pp. 223-30, 2008

Brognard, Sierecki, Gao, Newton: "PHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms." in: Molecular cell, Vol. 25, Issue 6, pp. 917-31, 2007

Gao, Furnari, Newton: "PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth." in: Molecular cell, Vol. 18, Issue 1, pp. 13-24, 2005

Images

Product Details anti-PHLPP1 Antibody Target Details PHLPP1 Application Details Handling References for anti-PHLPP1 Antibody (ABIN1449987) back to top
Supplier Images
Western Blotting (WB) image for anti-PHLPP1 antibody (PH Domain and Leucine Rich Repeat Protein Phosphatase 1) (N-Term) (ABIN1449987) Western blot analysis of PHLPP1 in SW480 cell lysate with PHLPP1 Antibody  at (A) 1 a...