anti-Human PHLPP1 antibody for Western Blotting

Recommended PHLPP1 Antibody (supplied by: Log in to see )

Antigen
PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1) Antibodies
  • PHLPP
  • PLEKHE1
  • SCOP
  • AI836256
  • Phlpp
  • Plekhe1
  • mKIAA0606
  • Scop
  • PH domain and leucine rich repeat protein phosphatase 1
  • PHLPP1
  • phlpp1
  • Phlpp1
Epitope
C-Term
11
6
3
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
33
15
11
Host
Rabbit
33
Clonality
Polyclonal
Application
Immunoprecipitation (IP), Western Blotting (WB)
26
9
6
5
4
4
2
2
1
Supplier
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Catalog No. ABIN151320
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1455724 ABIN392310 WB Rabbit Ig AA 531-560, N-Term Log in to see Polyclonal
3.1455724 ABIN2705120 WB Rabbit C-Term Log in to see Polyclonal
3.1455724 ABIN4904751 WB Rabbit IgG Log in to see Polyclonal
3.1455724 ABIN4345253 IP WB Rabbit Log in to see Polyclonal
3.1455724 ABIN1031510 ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN360194 EIA WB Rabbit Ig N-Term Log in to see Polyclonal 3
1 ABIN5556440 EIA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1804212 IHC (p) WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1453893 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN2334564 ELISA WB Rabbit IgG N-Term, PH domain Log in to see Polyclonal
1 ABIN2884713 ELISA WB Rabbit IgG AA 1194-1243 Log in to see Polyclonal
1 ABIN481040 IP WB Rabbit C-Term Log in to see Polyclonal
1 ABIN603650 ICC WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2334563 IC WB Rabbit C-Term, PH domain Log in to see Polyclonal
1 ABIN2580264 WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2451738 ICC IHC (p) WB Rabbit IgG AA 1192-1205, AA 1704-1717 Log in to see Polyclonal
1 ABIN2878332 WB Rabbit Log in to see Polyclonal
1 ABIN2861218 ICC IF ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1888364 ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN1840456 WB Rabbit AA 531-560 Log in to see Polyclonal

Top referenced anti-PHLPP1 antibody for Western Blotting

  • Human Polyclonal PHLPP1 Primary Antibody for IP, WB - ABIN151320 (5 Publications): Hirano, Nakamura, Yokota, Ono, Shigeno, Fujisawa, Shinjo, Ohnishi et al.: Depletion of Pleckstrin homology domain leucine-rich repeat protein phosphatases 1 and 2 by Bcr-Abl promotes chronic myelogenous leukemia cell proliferation through continuous phosphorylation of Akt ... in The Journal of biological chemistry 2009 (PubMed)

Similar anti-PHLPP1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 4 Antibodies 9 Antibodies
Immunocytochemistry (ICC) 3 Antibodies 4 Antibodies
Immunofluorescence (IF) 1 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 3 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies 5 Antibodies
Immunoprecipitation (IP) 4 Antibodies 7 Antibodies
Western Blotting (WB) 13 Antibodies 27 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunochromatography (IC) 1 Antibodies

General

Antigen PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1) Antibodies
Epitope C-Term
(11), (6), (3), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(33), (15), (11)
Host Rabbit
(33)
Clonality
Polyclonal
Application Immunoprecipitation (IP), Western Blotting (WB)
(26), (9), (6), (5), (4), (4), (2), (2), (1)
Pubmed 5 references available
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Product Details anti-PHLPP1 Antibody

Target Details PHLPP1 Application Details Handling ProductDetails: References for anti-PHLPP1 antibody (ABIN151320) Images
Specificity PHLPP antibody has been reported to cross reacts with beta-catenin, which may display 90 kDa band. We would not recommend using this specific antibody for assays where signals may not be notable between PHLPP1 and Beta-Catenin.
Purification Immunogen affinity purified
Immunogen The immunogen recognized by this antibody maps to a region between residue 1150 and the C-terminus (residue 1205) of human PH Domain and Leucine Rich Repeat Protein Phosphatase using the numbering given in entry NP_919431.1 (GeneID 23239).

Target Details PHLPP1

Product Details anti-PHLPP1 Antibody Application Details Handling ProductDetails: References for anti-PHLPP1 antibody (ABIN151320) Images back to top
Antigen
Alternative Name PHLPP (PHLPP1 Antibody Abstract)
Background Gene Symbol: PHLPP1
Molecular Weight Theoretical MW: 185 kDa
Gene ID 23239
NCBI Accession NP_919431
Research Area Apoptosis/Necrosis, Cancer
Pathways PI3K-Akt Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PHLPP1 Antibody Target Details PHLPP1 Handling ProductDetails: References for anti-PHLPP1 antibody (ABIN151320) Images back to top
Application Notes Western Blot 1:2000-1:10000, Immunoprecipitation 1-4 μg/mg of lysateWestern Blot: Beta catenin band in Western blot is relatively less intense than PHLPP band. We do not recommend this antibody in Immunohistochemistry, Immunocytochemistry and Immunofluorescence due to its cross reactivity to Beta Catenin. Use in Immunocytochemistry/immunofluorescence and Immunohistochemistry-Paraffin reported in scientific literature (PMID 25820252) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-PHLPP1 Antibody Target Details PHLPP1 Application Details ProductDetails: References for anti-PHLPP1 antibody (ABIN151320) Images back to top
Format Liquid
Concentration 0.2 mg/mL
Buffer TBS and 0.1 % BSA
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 4°C. Do not freeze.

ProductDetails: References for anti-PHLPP1 antibody (ABIN151320)

Product Details anti-PHLPP1 Antibody Target Details PHLPP1 Application Details Handling Images back to top
Product cited in:

Fatima, Wagstaff, Loveland, Jans: "Interactome of the negative regulator of nuclear import BRCA1-binding protein 2." in: Scientific reports, Vol. 5, pp. 9459, 2015 (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunohistochemistry (Paraffin-embedded Sections),Immunoprecipitation,Immunofluorescence

Huang, Chuang, Chou, Wang, Lee, Yang, Chiu, Kapuriya, Wang, Kulp, Chen: "Vitamin E facilitates the inactivation of the kinase Akt by the phosphatase PHLPP1." in: Science signaling, Vol. 6, Issue 267, pp. ra19, 2013

Liu, Yang, Bu, Liu, Ding, Li, Jia: "Cell-type-specific regulation of raft-associated Akt signaling." in: Cell death & disease, Vol. 2, pp. e145, 2011

Hirano, Nakamura, Yokota, Ono, Shigeno, Fujisawa, Shinjo, Ohnishi et al.: "Depletion of Pleckstrin homology domain leucine-rich repeat protein phosphatases 1 and 2 by Bcr-Abl promotes chronic myelogenous leukemia cell proliferation through continuous phosphorylation of Akt ..." in: The Journal of biological chemistry, Vol. 284, Issue 33, pp. 22155-65, 2009

Johnson, Gulhati, Arrieta, Wang, Uchida, Gao, Evers: "Curcumin inhibits proliferation of colorectal carcinoma by modulating Akt/mTOR signaling." in: Anticancer research, Vol. 29, Issue 8, pp. 3185-90, 2009

Images

Product Details anti-PHLPP1 Antibody Target Details PHLPP1 Application Details Handling ProductDetails: References for anti-PHLPP1 antibody (ABIN151320) back to top
Supplier Images
Western Blotting (WB) image for anti-PHLPP1 antibody (PH Domain and Leucine Rich Repeat Protein Phosphatase 1) (C-Term) (ABIN151320) Detection of Human and Mouse PHLPP by Western Blot (h & m) and Immunoprecipitation (h...