anti-Human PIK3CA antibody for Immunocytochemistry

Recommended PIK3CA Antibody (supplied by: Log in to see )

Antigen
Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
  • PI3-kinase
  • PI3K-alpha
  • pi3-k
  • pi3k
  • 6330412C24Rik
  • caPI3K
  • p110
  • p110alpha
  • CLOVE
  • CWS5
  • MCAP
  • MCM
  • MCMTC
  • PI3K
  • p110-alpha
  • C-P3K
  • phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
  • Phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
  • PIK3CA
  • GL50803_14855
  • pik3ca
  • LOC100337822
  • Pik3ca
Reactivity
Human
161
79
55
7
6
2
2
1
1
1
1
1
Host
Mouse
132
29
21
1
Clonality
Monoclonal
Conjugate
This PIK3CA antibody is un-conjugated
8
8
7
7
6
6
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
119
80
67
37
16
13
10
9
5
5
2
2
2
1
1
1
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Catalog No. ABIN969555
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4345590 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal 2
1 ABIN4345591 ICC IF IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1500200 ELISA FACS ICC IF WB Mouse IgG1 Log in to see 4F3
1 ABIN4345589 CyTOF ELISA FACS ICC IF WB Mouse IgG1 Log in to see 4F3
1 ABIN4880939 ELISA FACS ICC WB Mouse IgG1 Log in to see 4F3

General

Antigen Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
Reactivity Human
(161), (79), (55), (7), (6), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(132), (29), (21), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PIK3CA antibody is un-conjugated
(8), (8), (7), (7), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
(119), (80), (67), (37), (16), (13), (10), (9), (5), (5), (2), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-PIK3CA Antibody

Target Details PIK3CA Application Details Handling References for anti-PIK3CA antibody (ABIN969555) Images
Purification Purified
Immunogen Purified recombinant fragment of human PIK3CA expressed in E. Coli.
Clone 4F3
Isotype IgG1

Target Details PIK3CA

Product Details anti-PIK3CA Antibody Application Details Handling References for anti-PIK3CA antibody (ABIN969555) Images back to top
Antigen
Alternative Name PIK3CA (PIK3CA Antibody Abstract)
Background Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.
Molecular Weight 124 kDa
Gene ID 5290
HGNC 5290
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-PIK3CA Antibody Target Details PIK3CA Handling References for anti-PIK3CA antibody (ABIN969555) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details References for anti-PIK3CA antibody (ABIN969555) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-PIK3CA antibody (ABIN969555)

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling Images back to top
Product cited in:

Edwards, Witherspoon, Wang, Afrasiabi, Pham, Birnbaumer, Lipkin: "Epigenetic repression of DNA mismatch repair by inflammation and hypoxia in inflammatory bowel disease-associated colorectal cancer." in: Cancer research, Vol. 69, Issue 16, pp. 6423-9, 2009

Pang, Flinn, Patsialou, Wyckoff, Roussos, Wu, Pozzuto, Goswami, Condeelis, Bresnick, Segall, Backer: "Differential enhancement of breast cancer cell motility and metastasis by helical and kinase domain mutations of class IA phosphoinositide 3-kinase." in: Cancer research, Vol. 69, Issue 23, pp. 8868-76, 2009

Michelucci, Di Cristofano, Lami, Collecchi, Caligo, Decarli, Leopizzi, Aretini, Bertacca, Porta, Ricci, Della Rocca, Stanta, Bevilacqua, Cavazzana: "PIK3CA in breast carcinoma: a mutational analysis of sporadic and hereditary cases." in: Diagnostic molecular pathology : the American journal of surgical pathology, part B, Vol. 18, Issue 4, pp. 200-5, 2009

Images

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling References for anti-PIK3CA antibody (ABIN969555) back to top
Supplier Images
ELISA image for anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) antibody (ABIN969555) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) antibody (ABIN969555) Figure 2: Flow cytometric analysis of Jurkat cells using PIK3CA mouse mAb (green) and...
Immunocytochemistry (ICC) image for anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) antibody (ABIN969555) Figure 3: Immunofluorescence analysis of HeLa cells using PIK3CA mouse mAb (green) . ...
Western Blotting (WB) image for anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) antibody (ABIN969555) Figure 4: Western blot analysis using PIK3CA mAb against human PIK3CA (AA: 881-1068) ...
Western Blotting (WB) image for anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) antibody (ABIN969555) Figure 5: Western blot analysis using PIK3CA mAb against HEK293 (1) and PIK3CA (AA: 8...