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|Application / Reactivity||Human|
|BioImaging (BI)||2 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies|
|Flow Cytometry (FACS)||11 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Immunofluorescence (IF)||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||75 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||31 Antibodies|
|Immunoprecipitation (IP)||8 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Western Blotting (WB)||89 Antibodies|
|Antigen||Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies|
|Conjugate||This PIK3CA antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-PIK3CA AntibodyTarget Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN969555) Images
|Immunogen||Purified recombinant fragment of human PIK3CA expressed in E. coli.|
Target Details PIK3CAProduct Details anti-PIK3CA Antibody Application Details Handling References for anti-PIK3CA Antibody (ABIN969555) Images back to top
|Alternative Name||PIK3CA (PIK3CA Antibody Abstract)|
|Background||Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.|
|Molecular Weight||124 kDa|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-PIK3CA Antibody Target Details PIK3CA Handling References for anti-PIK3CA Antibody (ABIN969555) Images back to top
ELISA: 1/10000, WB: 1/500 - 1/2000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PIK3CA Antibody Target Details PIK3CA Application Details References for anti-PIK3CA Antibody (ABIN969555) Images back to top
|Buffer||PBS with 0.05 % sodium azide and 0.5 % protein stabilizer|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 4 °C or at -20 °C for long term.|
References for anti-PIK3CA Antibody (ABIN969555)Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling Images back to top
|Product cited in:||
Pang, Flinn, Patsialou et al.: "Differential enhancement of breast cancer cell motility and metastasis by helical and kinase domain mutations of class IA phosphoinositide 3-kinase." in: Cancer research, Vol. 69, Issue 23, pp. 8868-76, 2009 (PubMed).
Michelucci, Di Cristofano, Lami et al.: "PIK3CA in breast carcinoma: a mutational analysis of sporadic and hereditary cases." in: Diagnostic molecular pathology : the American journal of surgical pathology, part B, Vol. 18, Issue 4, pp. 200-5, 2009 (PubMed).
Edwards, Witherspoon, Wang et al.: "Epigenetic repression of DNA mismatch repair by inflammation and hypoxia in inflammatory bowel disease-associated colorectal cancer." in: Cancer research, Vol. 69, Issue 16, pp. 6423-9, 2009 (PubMed).
ImagesProduct Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN969555) back to top