anti-Human PIK3CA antibody for Immunofluorescence

Recommended PIK3CA Antibody (supplied by: Log in to see )

Antigen
Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
  • PI3-kinase
  • PI3K-alpha
  • pi3-k
  • pi3k
  • 6330412C24Rik
  • caPI3K
  • p110
  • p110alpha
  • CLOVE
  • CWS5
  • MCAP
  • MCM
  • MCMTC
  • PI3K
  • p110-alpha
  • C-P3K
  • phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
  • Phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
  • PIK3CA
  • GL50803_14855
  • pik3ca
  • LOC100337822
  • Pik3ca
Epitope
AA 101-300
29
18
16
14
12
9
8
8
5
5
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
161
79
54
7
6
2
2
1
1
1
1
1
Host
Mouse
132
29
21
1
Clonality
Monoclonal
Conjugate
This PIK3CA antibody is un-conjugated
8
8
7
7
6
6
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
119
80
68
37
17
13
10
8
6
5
2
2
2
1
1
1
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Catalog No. ABIN968519
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.149872 ABIN968518 IF WB Mouse IgG1 AA 101-300 Log in to see 19-PI3 5
12.149872 ABIN1108651 EIA FACS IF WB Mouse IgG1 Log in to see 4F3 2
12.149872 ABIN4345590 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal 2
12.149872 ABIN4345589 CyTOF ELISA FACS ICC IF WB Mouse IgG1 Log in to see 4F3
12.149872 ABIN2682476 IF IF/ICC WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN4345591 ICC IF IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1500200 ELISA FACS ICC IF WB Mouse IgG1 Log in to see 4F3
1 ABIN5585687 IF IHC (p) Rabbit IgG Log in to see Polyclonal

General

Antigen Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
Epitope AA 101-300
(29), (18), (16), (14), (12), (9), (8), (8), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(161), (79), (54), (7), (6), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(132), (29), (21), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PIK3CA antibody is un-conjugated
(8), (8), (7), (7), (6), (6), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(119), (80), (68), (37), (17), (13), (10), (8), (6), (5), (2), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-PIK3CA Antibody

Target Details PIK3CA Application Details Handling References for anti-PIK3CA antibody (ABIN968519) Images
Cross-Reactivity Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human PI3-Kinase p110alpha aa. 101-300
Clone 19-PI3
Isotype IgG1

Target Details PIK3CA

Product Details anti-PIK3CA Antibody Application Details Handling References for anti-PIK3CA antibody (ABIN968519) Images back to top
Antigen
Alternative Name PI3-Kinase p110 alpha (PIK3CA Antibody Abstract)
Background Phosphatidylinositol 3 (PI3) -kinase participates in insulin-stimulated glucose uptake, PDGF-induced membrane ruffling, and G-protein receptor signaling. It exists as a heterodimer of 85 kDa (p85) and 110 kDa (p110) subunits. The p85 subunit associates with and serves as a substrate for activated growth factor receptor tyrosine kinases. p85 regulates the p110 catalytic subunit by acting as the link between PI3-kinase and the ligand-activated receptor. Four isoforms of p110 have been identified (alpha, beta, gamma, and delta). The p110alpha isoform contains an N-terminal region involved in p85 binding and a C-terminal kinase domain. p85/p110alpha-type PI kinase phosphorylates the D-3 and D-4 position of the inositol ring of PI, thereby producing PtdIns(3)P, PtdIns(3,4)P[2], PtdIns(3,4,5)P[3], PtdIns(4)P, and PtdIns(4,5) P[2]. During induction of chemotaxis by the chemokine SDF-1alpha, PI3-kinase regulates adhesion and ERM protein redistribution in the lymphocyte plasma membrane. In addition, PI3-kinases activate other signaling molecules, such as p70 S6 kinase and Akt/protein kinase B. Thus, p85/p110alpha-type PI kinase is a ubiquitously expressed kinase that is involved in a variety of cell signaling cascades.
Molecular Weight 110 kDa
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-PIK3CA Antibody Target Details PIK3CA Handling References for anti-PIK3CA antibody (ABIN968519) Images back to top
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details References for anti-PIK3CA antibody (ABIN968519) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-PIK3CA antibody (ABIN968519)

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling Images back to top
Product cited in:

Funaki, Katagiri, Kanda, Anai, Nawano, Ogihara, Inukai, Fukushima, Ono, Yazaki, Kikuchi, Oka, Asano: "p85/p110-type phosphatidylinositol kinase phosphorylates not only the D-3, but also the D-4 position of the inositol ring." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 22019-24, 1999

Vicente-Manzanares, Rey, Jones, Sancho, Mellado, Rodriguez-Frade, del Pozo, Yáñez-Mó, de Ana, Martínez-A, Mérida, Sánchez-Madrid: "Involvement of phosphatidylinositol 3-kinase in stromal cell-derived factor-1 alpha-induced lymphocyte polarization and chemotaxis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 7, pp. 4001-12, 1999

Katagiri, Asano, Ishihara, Inukai, Shibasaki, Kikuchi, Yazaki, Oka: "Overexpression of catalytic subunit p110alpha of phosphatidylinositol 3-kinase increases glucose transport activity with translocation of glucose transporters in 3T3-L1 adipocytes." in: The Journal of biological chemistry, Vol. 271, Issue 29, pp. 16987-90, 1996

Hu, Klippel, Muslin, Fantl, Williams: "Ras-dependent induction of cellular responses by constitutively active phosphatidylinositol-3 kinase." in: Science (New York, N.Y.), Vol. 268, Issue 5207, pp. 100-2, 1995

Volinia, Hiles, Ormondroyd, Nizetic, Antonacci, Rocchi, Waterfield: "Molecular cloning, cDNA sequence, and chromosomal localization of the human phosphatidylinositol 3-kinase p110 alpha (PIK3CA) gene." in: Genomics, Vol. 24, Issue 3, pp. 472-7, 1995

Images

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling References for anti-PIK3CA antibody (ABIN968519) back to top
Supplier Images
Western Blotting (WB) image for anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (AA 101-300) antibody (ABIN968519) Western blot analysis of PI3-Kinase p110alpha on Jurkat lysate. Lane 1: 1:250, lane 2...
Immunofluorescence (IF) image for anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (AA 101-300) antibody (ABIN968519) Immunofluorescent staining of NIH-3T3 cells.
 image for anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (AA 101-300) antibody (ABIN968519) anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (AA 101-300) antibody (Image 3)
Western Blotting (WB) image for anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (AA 101-300) antibody (ABIN968519) anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (AA 101-300) antibody (Image 4)