anti-Human PIK3CA antibody for Immunohistochemistry

Recommended PIK3CA Antibody (supplied by: Log in to see )

Antigen
Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
  • PI3-kinase
  • PI3K-alpha
  • pi3-k
  • pi3k
  • 6330412C24Rik
  • caPI3K
  • p110
  • p110alpha
  • CLOVE
  • CWS5
  • MCAP
  • MCM
  • MCMTC
  • PI3K
  • p110-alpha
  • C-P3K
  • phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
  • Phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
  • PIK3CA
  • GL50803_14855
  • pik3ca
  • LOC100337822
  • Pik3ca
Epitope
Internal Region
29
16
16
14
12
9
5
5
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
158
61
55
7
6
2
2
1
1
1
1
1
Host
Goat
111
30
20
1
Clonality
Polyclonal
Conjugate
This PIK3CA antibody is un-conjugated
7
6
6
6
5
5
1
1
1
1
1
1
1
1
1
1
1
Method Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
100
79
67
36
15
13
10
9
6
4
2
2
2
1
1
1
Options
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Catalog No. ABIN334459
$ 326.89
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.603206 ABIN188684 ELISA IHC Goat Log in to see Polyclonal 2
9.603206 ABIN2706806 IHC WB Rabbit Center Log in to see Polyclonal
9.603206 ABIN2466113 IHC ELISA Goat Internal Region Log in to see Polyclonal
9.603206 ABIN1874129 IHC WB Rabbit IgG Log in to see Polyclonal
9.603206 ABIN4345592 IHC Mouse IgG2a Log in to see 6D9
9.603206 ABIN3028924 IHC ELISA WB Rabbit Ig Fraction AA 504-533 Log in to see Polyclonal
1 ABIN967708 BI IP IHC WB Mouse IgG2a AA 562-724 Log in to see 4-PI3 5
1 ABIN967707 BI IP IHC WB Mouse IgG2a AA 562-724 Log in to see 4-PI3 5
1 ABIN4345590 ICC IF IHC IHC (p) Rabbit IgG Log in to see Polyclonal 2
1 ABIN3186429 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN593539 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN4345591 ICC IF IHC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1874130 IP IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN2564537 FACS IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN1453773 IHC ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
1 ABIN1953193 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG Tyr317 Log in to see Polyclonal
1 ABIN1958838 IHC ELISA WB PE Rabbit IgG Tyr317 Log in to see Polyclonal
1 ABIN1954065 IHC ELISA WB APC Rabbit IgG Tyr317 Log in to see Polyclonal
1 ABIN1955315 IHC ELISA WB Biotin Rabbit IgG Tyr317 Log in to see Polyclonal
1 ABIN2564536 IHC ELISA Goat IgG Internal Region Log in to see Polyclonal

Top referenced anti-PIK3CA antibody for Immunohistochemistry

Similar anti-PIK3CA Antibodies

Application / Reactivity Human
BioImaging (BI) 2 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
ELISA 68 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 15 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 9 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 79 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 35 Antibodies
Immunoprecipitation (IP) 10 Antibodies
In Vivo Imaging (IVI) 1 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 98 Antibodies

General

Antigen Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
Epitope Internal Region
(29), (16), (16), (14), (12), (9), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(158), (61), (55), (7), (6), (2), (2), (1), (1), (1), (1), (1)
Host Goat
(111), (30), (20), (1)
Clonality
Polyclonal
Conjugate This PIK3CA antibody is un-conjugated
(7), (6), (6), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Method Type Peptide ELISA
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(100), (79), (67), (36), (15), (13), (10), (9), (6), (4), (2), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-PIK3CA Antibody

Target Details PIK3CA Application Details Handling ProductDetails: References for anti-PIK3CA antibody (ABIN334459) Images
Blocking Peptide Blocking peptide for this product available: ABIN370308
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen Peptide with sequence C-HQWLKDKNKGEIYD, from the internal region of the protein sequence according to NP_006209.2.

Target Details PIK3CA

Product Details anti-PIK3CA Antibody Application Details Handling ProductDetails: References for anti-PIK3CA antibody (ABIN334459) Images back to top
Antigen
Alternative Name PIK3CA / P110alpha (PIK3CA Antibody Abstract)
Background Synonyms: PIK3CA, p110-alpha, phosphoinositide-3-kinase, catalytic, alpha polypeptide, MGC142161, MGC142163, PI3K, PI3-kinase p110 subunit alpha, PtdIns-3-kinase p110, phosphatidylinositol 3-kinase, catalytic, 110-KD, alpha, phosphatidylinositol 3-kinase, catalytic, alpha polypeptide, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit, alpha isoform
Gene ID 18706
NCBI Accession NP_006209
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals

Application Details

Product Details anti-PIK3CA Antibody Target Details PIK3CA Handling ProductDetails: References for anti-PIK3CA antibody (ABIN334459) Images back to top
Application Notes ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments in lysates of cell lines A431, HeLa and Jurkat gave no specific signal but low background (at antibody concentration up to 1 µg/mL).
IHC: In paraffin embedded Human Brain (Cerebral Cortex) shows cytoplasm stained in the cell bodies. Recommended concentration: 2-3 µg/mL. This antibody was successfully used on Human epithelial ovarian carcinoma: Abubaker et al. Mol Cancer. 2009 Jul 28,8:51. (PMID: 19638206).
Restrictions For Research Use only

Handling

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details ProductDetails: References for anti-PIK3CA antibody (ABIN334459) Images back to top
Format Liquid
Concentration 100µg specific antibody in 200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.

ProductDetails: References for anti-PIK3CA antibody (ABIN334459)

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling Images back to top
Product cited in:

Abubaker, Bavi, Al-Haqawi, Jehan, Munkarah, Uddin, Al-Kuraya: "PIK3CA alterations in Middle Eastern ovarian cancers." in: Molecular cancer, Vol. 8, pp. 51, 2009

Background publications

López-Knowles, Hernández, Malats, Kogevinas, Lloreta, Carrato, Tardón, Serra, Real: "PIK3CA mutations are an early genetic alteration associated with FGFR3 mutations in superficial papillary bladder tumors." in: Cancer research, Vol. 66, Issue 15, pp. 7401-4, 2006

Images

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling ProductDetails: References for anti-PIK3CA antibody (ABIN334459) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (Internal Region) (ABIN334459) Antibody (2.5µg/ml) staining of paraffin embedded Human Brain (Cerebellar Cortex). St...