anti-Mouse (Murine) PIK3CA antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended PIK3CA Antibody (supplied by: Log in to see )

Antigen
Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
  • PI3-kinase
  • PI3K-alpha
  • pi3-k
  • pi3k
  • 6330412C24Rik
  • caPI3K
  • p110
  • p110alpha
  • CLOVE
  • CWS5
  • MCAP
  • MCM
  • MCMTC
  • PI3K
  • p110-alpha
  • C-P3K
  • phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
  • Phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
  • PIK3CA
  • GL50803_14855
  • pik3ca
  • LOC100337822
  • Pik3ca
Epitope
AA 1010-1050
30
15
14
12
9
8
8
5
5
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
161
60
54
7
6
2
2
1
1
1
1
1
Host
Rabbit
113
30
21
1
Clonality
Polyclonal
Conjugate
This PIK3CA antibody is un-conjugated
7
7
6
6
5
5
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
101
80
68
36
17
12
10
9
6
5
2
2
2
1
1
1
Options
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Catalog No. ABIN677198
$ 307.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN677205 IF (p) FITC Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN911090 IF (p) Alexa Fluor 350 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN911091 IF (p) Alexa Fluor 488 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN911092 IF (p) Alexa Fluor 555 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN911093 IF (p) Alexa Fluor 647 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN2808910 IF (p) Alexa Fluor 594 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN677201 IF (p) Cy3 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN677202 IF (p) Cy5 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN677203 IF (p) Cy5.5 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN677204 IF (p) Cy7 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN5008804 IF (p) Alexa Fluor 750 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN5008803 IF (p) Alexa Fluor 680 Rabbit IgG AA 1010-1050 Log in to see Polyclonal

General

Antigen Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
Epitope AA 1010-1050
(30), (15), (14), (12), (9), (8), (8), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(161), (60), (54), (7), (6), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(113), (30), (21), (1)
Clonality
Polyclonal
Conjugate This PIK3CA antibody is un-conjugated
(7), (7), (6), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(101), (80), (68), (36), (17), (12), (10), (9), (6), (5), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PIK3CA Antibody

Target Details PIK3CA Application Details Handling References for anti-PIK3CA antibody (ABIN677198) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human PI3KCA
Isotype IgG

Target Details PIK3CA

Product Details anti-PIK3CA Antibody Application Details Handling References for anti-PIK3CA antibody (ABIN677198) Images back to top
Antigen
Alternative Name Pi3kca (PIK3CA Antibody Abstract)
Background

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS.

Subcellular location: Cytoplasm, Membrane bound

Synonyms: MCM, CWS5, MCAP, PI3K, CLOVE, MCMTC, p11-alpha, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PtdIns-3-kinase subunit alpha, Phosphatidylinositol 4,5-bisphosphate 3-kinase 11 kDa catalytic subunit alpha, PtdIns-3-kinase subunit p11-alpha, p11alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA, PIK3CA

Gene ID 5290
UniProt P42336
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Inositol Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-PIK3CA Antibody Target Details PIK3CA Handling References for anti-PIK3CA antibody (ABIN677198) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details References for anti-PIK3CA antibody (ABIN677198) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-PIK3CA antibody (ABIN677198)

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling Images back to top
Product cited in:

Paul-Samojedny, Suchanek, Borkowska, Pude?ko, Owczarek, Kowalczyk, Machnik, Fila-Dani?ow, Kowalski: "Knockdown of AKT3 (PKB?) and PI3KCA suppresses cell viability and proliferation and induces the apoptosis of glioblastoma multiforme T98G cells." in: BioMed research international, Vol. 2014, pp. 768181, 2014

Images

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling References for anti-PIK3CA antibody (ABIN677198) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (AA 1010-1050) antibody (ABIN677198) Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boi...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (AA 1010-1050) antibody (ABIN677198) Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boil...
Western Blotting (WB) image for anti-Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) (AA 1010-1050) antibody (ABIN677198) HeLa Cell lysates probed with PI3KCA Polyclonal Antibody, unconjugated at 1:300 over...