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anti-Rat (Rattus) PIK3CA antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended PIK3CA Antibody (supplied by: Log in to see )

Antigen
Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
  • PI3-kinase
  • PI3K-alpha
  • pi3-k
  • pi3k
  • 6330412C24Rik
  • caPI3K
  • p110
  • p110alpha
  • CLOVE
  • CWS5
  • MCAP
  • MCM
  • MCMTC
  • PI3K
  • p110-alpha
  • C-P3K
  • phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha
  • Phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • phosphoinositide-3-kinase, catalytic, alpha polypeptide
  • phosphatidylinositol 3-kinase, catalytic, alpha polypeptide
  • PIK3CA
  • GL50803_14855
  • pik3ca
  • LOC100337822
  • Pik3ca
Epitope
AA 1010-1050
34
16
15
14
13
9
4
3
3
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
152
52
45
7
4
2
1
1
1
Host
Rabbit
106
26
23
Clonality
Polyclonal
Conjugate
This PIK3CA antibody is un-conjugated
6
6
6
6
5
5
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
92
80
70
30
26
12
8
8
7
5
2
2
1
1
1
Options
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Catalog No. ABIN677198
$ 307.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2808910 FACS IF (p) Alexa Fluor 594 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN677205 FACS IF (p) FITC Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN677204 FACS IF (p) Cy7 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN677202 FACS IF (p) Cy5 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN677203 FACS IF (p) Cy5.5 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN677201 FACS IF (p) Cy3 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN911093 FACS IF (p) Alexa Fluor 647 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN911090 FACS IF (p) Alexa Fluor 350 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN911091 FACS IF (p) Alexa Fluor 488 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN911092 FACS IF (p) Alexa Fluor 555 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN5008803 FACS IF (p) Alexa Fluor 680 Rabbit IgG AA 1010-1050 Log in to see Polyclonal
1 ABIN5008804 FACS IF (p) Alexa Fluor 750 Rabbit IgG AA 1010-1050 Log in to see Polyclonal

Top referenced anti-PIK3CA antibody for Immunofluorescence (Paraffin-embedded Sections)

  • Human Polyclonal PIK3CA Primary Antibody for FACS, IF (p) - ABIN677198 (1 Publications): Paul-Samojedny, Suchanek, Borkowska, Pude?ko, Owczarek, Kowalczyk, Machnik, Fila-Dani?ow, Kowalski: Knockdown of AKT3 (PKB?) and PI3KCA suppresses cell viability and proliferation and induces the apoptosis of glioblastoma multiforme T98G cells. in BioMed research international 2014 (PubMed)

Similar anti-PIK3CA Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
BioImaging (BI) 2 Antibodies 2 Antibodies
ELISA 6 Antibodies 8 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 16 Antibodies 16 Antibodies
Immunofluorescence (IF) 2 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 16 Antibodies 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies 13 Antibodies
Immunoprecipitation (IP) 4 Antibodies 4 Antibodies
Western Blotting (WB) 26 Antibodies
Immunocytochemistry (ICC) 1 Antibodies

General

Antigen Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide (PIK3CA) Antibodies
Epitope AA 1010-1050
(34), (16), (15), (14), (13), (9), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(152), (52), (45), (7), (4), (2), (1), (1), (1)
Host Rabbit
(106), (26), (23)
Clonality
Polyclonal
Conjugate This PIK3CA antibody is un-conjugated
(6), (6), (6), (6), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(92), (80), (70), (30), (26), (12), (8), (8), (7), (5), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-PIK3CA Antibody

Target Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN677198) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human PI3KCA
Isotype IgG

Target Details PIK3CA

Product Details anti-PIK3CA Antibody Application Details Handling References for anti-PIK3CA Antibody (ABIN677198) Images back to top
Antigen
Alternative Name Pi3kca (PIK3CA Antibody Abstract)
Background

Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS.

Subcellular location: Cytoplasm, Membrane bound


Synonyms: MCM, CWS5, MCAP, PI3K, CLOVE, MCMTC, p11-alpha, Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform, PI3-kinase subunit alpha, PI3K-alpha, PI3Kalpha, PtdIns-3-kinase subunit alpha, Phosphatidylinositol 4,5-bisphosphate 3-kinase 11 kDa catalytic subunit alpha, PtdIns-3-kinase subunit p11-alpha, p11alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, Serine/threonine protein kinase PIK3CA, PIK3CA
Gene ID 5290
UniProt P42336
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-PIK3CA Antibody Target Details PIK3CA Handling References for anti-PIK3CA Antibody (ABIN677198) Images back to top
Application Notes WB(1:100-1000), FCM(1:20-100), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details References for anti-PIK3CA Antibody (ABIN677198) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-PIK3CA Antibody (ABIN677198)

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling Images back to top
Product cited in:

Paul-Samojedny, Suchanek, Borkowska, Pude?ko, Owczarek, Kowalczyk, Machnik, Fila-Dani?ow, Kowalski: "Knockdown of AKT3 (PKB?) and PI3KCA suppresses cell viability and proliferation and induces the apoptosis of glioblastoma multiforme T98G cells." in: BioMed research international, Vol. 2014, pp. 768181, 2014

Images

Product Details anti-PIK3CA Antibody Target Details PIK3CA Application Details Handling References for anti-PIK3CA Antibody (ABIN677198) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (AA 1010-1050) (ABIN677198) Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boi...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (AA 1010-1050) (ABIN677198) Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boil...
Western Blotting (WB) image for anti-PIK3CA antibody (Phosphoinositide-3-Kinase, Catalytic, alpha Polypeptide) (AA 1010-1050) (ABIN677198) HeLa Cell lysates probed with PI3KCA Polyclonal Antibody, unconjugated at 1:300 over...