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|Application / Reactivity||Human|
|Dot Blot (DB)||18 Antibodies|
|ELISA (Capture)||1 Antibodies|
|Enzyme Immunoassay (EIA)||6 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies|
|Immunofluorescence (IF)||16 Antibodies|
|Immunohistochemistry (IHC)||42 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||19 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Western Blotting (WB)||124 Antibodies|
|Antigen||Phosphoinositide-3-Kinase, Catalytic, gamma Polypeptide (PIK3CG) Antibodies|
|Conjugate||This PIK3CG antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA)
|5 references available|
|Supplier||Log in to see|
Product Details anti-PIK3CG AntibodyTarget Details PIK3CG Application Details Handling References for anti-PIK3CG Antibody (ABIN358396) Images
|Specificity||This antibody detects PIK3CG pSer1100.|
|Purification||Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1100 of human PIK3CG.|
Target Details PIK3CGProduct Details anti-PIK3CG Antibody Application Details Handling References for anti-PIK3CG Antibody (ABIN358396) Images back to top
|Alternative Name||PIK3CG (PIK3CG Antibody Abstract)|
|Background||PIK3CG belongs to the pi3/pi4-kinase family of proteins. It is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene for this protein is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias.Synonyms: 5-bisphosphate 3-kinase catalytic subunit gamma isoform, PI3-kinase subunit gamma, PI3K-gamma, Phosphatidylinositol-4, PtdIns-3-kinase subunit p110-gamma, p120-PI3K|
|Molecular Weight||126454 Da|
|Gene ID||5294, 9606|
|Research Area||Kinases/Phosphatases, Signaling, Cancer|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling|
Application DetailsProduct Details anti-PIK3CG Antibody Target Details PIK3CG Handling References for anti-PIK3CG Antibody (ABIN358396) Images back to top
ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PIK3CG Antibody Target Details PIK3CG Application Details References for anti-PIK3CG Antibody (ABIN358396) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-PIK3CG Antibody (ABIN358396)Product Details anti-PIK3CG Antibody Target Details PIK3CG Application Details Handling Images back to top
Arai, Chikaraishi, Omura et al.: "First total synthesis of the antitumor compound (-)-kazusamycin A and absolute structure determination." in: Organic letters, Vol. 6, Issue 17, pp. 2845-8, 2004 (PubMed).
Reddy, Lin, Huang et al.: "The IL-1 receptor accessory protein is essential for PI 3-kinase recruitment and activation." in: Biochemical and biophysical research communications, Vol. 316, Issue 4, pp. 1022-8, 2004 (PubMed).
Théberge, Mehdi, Pandey et al.: "Prolongation of insulin-induced activation of mitogen-activated protein kinases ERK 1/2 and phosphatidylinositol 3-kinase by vanadyl sulfate, a protein tyrosine phosphatase inhibitor." in: Archives of biochemistry and biophysics, Vol. 420, Issue 1, pp. 9-17, 2003 (PubMed).
Osaki, Kase, Adachi et al.: "Inhibition of the PI3K-Akt signaling pathway enhances the sensitivity of Fas-mediated apoptosis in human gastric carcinoma cell line, MKN-45." in: Journal of cancer research and clinical oncology, Vol. 130, Issue 1, pp. 8-14, 2003 (PubMed).
Khan, Di Cello, Nath et al.: "Human immunodeficiency virus type 1 tat-mediated cytotoxicity of human brain microvascular endothelial cells." in: Journal of neurovirology, Vol. 9, Issue 6, pp. 584-93, 2003 (PubMed).
ImagesProduct Details anti-PIK3CG Antibody Target Details PIK3CG Application Details Handling References for anti-PIK3CG Antibody (ABIN358396) back to top