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anti-Human PIK3CG antibody for Enzyme Immunoassay

Recommended PIK3CG Antibody (supplied by: Log in to see )

Antigen
Phosphoinositide-3-Kinase, Catalytic, gamma Polypeptide (PIK3CG) Antibodies
  • 5830428L06Rik
  • p110gamma
  • p120-PI3K
  • PI3CG
  • pi3k
  • Pi3k
  • PI3K
  • PI3Kgamma
  • PIK3
  • wu:fb15d07
  • zgc:77033
Epitope
pSer1100
29
23
12
11
8
7
2
2
2
1
1
1
1
1
1
Reactivity
Human
156
19
12
3
2
1
1
Host
Rabbit
114
30
12
1
Clonality
Polyclonal
Conjugate
This PIK3CG antibody is un-conjugated
10
10
10
10
10
10
Application
Enzyme Immunoassay (EIA)
123
91
42
19
18
16
5
3
2
2
1
Supplier
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Catalog No. ABIN358396
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN360452 EIA IHC (p) WB Rabbit Ig AA 1073-1102, C-Term Log in to see Polyclonal 5
0.0008833851 ABIN360713 EIA IHC (p) WB Rabbit Ig Middle Region Log in to see Polyclonal 5
0.0008833851 ABIN360712 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 5
0.0008833851 ABIN360714 EIA WB Rabbit Ig Middle Region Log in to see Polyclonal 3
0.0008833851 ABIN374260 EIA WB Goat C-Term Log in to see Polyclonal 1

Top referenced anti-PIK3CG antibody for Enzyme Immunoassay

Similar anti-PIK3CG Antibodies

Application / Reactivity Human
Dot Blot (DB) 18 Antibodies
ELISA 92 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 16 Antibodies
Immunohistochemistry (IHC) 42 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 124 Antibodies

General

Antigen Phosphoinositide-3-Kinase, Catalytic, gamma Polypeptide (PIK3CG) Antibodies
Epitope pSer1100
(29), (23), (12), (11), (8), (7), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(156), (19), (12), (3), (2), (1), (1)
Host Rabbit
(114), (30), (12), (1)
Clonality
Polyclonal
Conjugate This PIK3CG antibody is un-conjugated
(10), (10), (10), (10), (10), (10)
Application Enzyme Immunoassay (EIA)
(123), (91), (42), (19), (18), (16), (5), (3), (2), (2), (1)
Pubmed 5 references available
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Product Details anti-PIK3CG Antibody

Target Details PIK3CG Application Details Handling References for anti-PIK3CG Antibody (ABIN358396) Images
Specificity This antibody detects PIK3CG pSer1100.
Purification Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1100 of human PIK3CG.
Isotype Ig

Target Details PIK3CG

Product Details anti-PIK3CG Antibody Application Details Handling References for anti-PIK3CG Antibody (ABIN358396) Images back to top
Antigen
Alternative Name PIK3CG (PIK3CG Antibody Abstract)
Background PIK3CG belongs to the pi3/pi4-kinase family of proteins. It is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene for this protein is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias.Synonyms: 5-bisphosphate 3-kinase catalytic subunit gamma isoform, PI3-kinase subunit gamma, PI3K-gamma, Phosphatidylinositol-4, PtdIns-3-kinase subunit p110-gamma, p120-PI3K
Molecular Weight 126454 Da
Gene ID 5294, 9606
UniProt P48736
Research Area Kinases/Phosphatases, Signaling, Cancer
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling

Application Details

Product Details anti-PIK3CG Antibody Target Details PIK3CG Handling References for anti-PIK3CG Antibody (ABIN358396) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PIK3CG Antibody Target Details PIK3CG Application Details References for anti-PIK3CG Antibody (ABIN358396) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-PIK3CG Antibody (ABIN358396)

Product Details anti-PIK3CG Antibody Target Details PIK3CG Application Details Handling Images back to top
Background publications

Reddy, Lin, Huang, Samanta, Liao: "The IL-1 receptor accessory protein is essential for PI 3-kinase recruitment and activation." in: Biochemical and biophysical research communications, Vol. 316, Issue 4, pp. 1022-8, 2004

Arai, Chikaraishi, Omura, Kuwajima: "First total synthesis of the antitumor compound (-)-kazusamycin A and absolute structure determination." in: Organic letters, Vol. 6, Issue 17, pp. 2845-8, 2004

Osaki, Kase, Adachi, Takeda, Hashimoto, Ito: "Inhibition of the PI3K-Akt signaling pathway enhances the sensitivity of Fas-mediated apoptosis in human gastric carcinoma cell line, MKN-45." in: Journal of cancer research and clinical oncology, Vol. 130, Issue 1, pp. 8-14, 2003

Théberge, Mehdi, Pandey, Srivastava: "Prolongation of insulin-induced activation of mitogen-activated protein kinases ERK 1/2 and phosphatidylinositol 3-kinase by vanadyl sulfate, a protein tyrosine phosphatase inhibitor." in: Archives of biochemistry and biophysics, Vol. 420, Issue 1, pp. 9-17, 2003

Khan, Di Cello, Nath, Kim: "Human immunodeficiency virus type 1 tat-mediated cytotoxicity of human brain microvascular endothelial cells." in: Journal of neurovirology, Vol. 9, Issue 6, pp. 584-93, 2003

Images

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Supplier Images
 image for anti-PIK3CG antibody (Phosphoinositide-3-Kinase, Catalytic, gamma Polypeptide) (pSer1100) (ABIN358396) anti-Phosphoinositide-3-Kinase, Catalytic, gamma Polypeptide (PIK3CG) (pSer1100) antibody