anti-Human PIK3CG antibody for Enzyme Immunoassay

Recommended PIK3CG Antibody (supplied by: Log in to see )

Antigen
Phosphoinositide-3-Kinase, Catalytic, gamma Polypeptide (PIK3CG) Antibodies
  • wu:fb15d07
  • zgc:77033
  • pi3k
  • PI3CG
  • PI3K
  • PI3Kgamma
  • PIK3
  • Pi3k
  • p120-PI3K
  • 5830428L06Rik
  • p110gamma
  • phosphoinositide-3-kinase, catalytic, gamma polypeptide
  • phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit gamma
  • pik3cg
  • PIK3CG
  • LOC100344144
  • Pik3cg
Epitope
pSer1100
26
10
9
9
8
7
5
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
149
22
14
4
1
Host
Rabbit
114
32
3
1
Clonality
Polyclonal
Conjugate
This PIK3CG antibody is un-conjugated
9
9
9
9
9
9
Application
Enzyme Immunoassay (EIA)
124
80
43
23
17
16
4
3
2
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN358396
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN360452 EIA IHC (p) WB Rabbit Ig AA 1073-1102, C-Term Log in to see Polyclonal 5
1 ABIN360714 EIA WB Rabbit Ig Middle Region Log in to see Polyclonal 3
1 ABIN5611055 EIA IHC (p) WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
1 ABIN5611056 EIA IHC (p) WB Rabbit Ig Fraction Middle Region Log in to see Polyclonal

General

Antigen Phosphoinositide-3-Kinase, Catalytic, gamma Polypeptide (PIK3CG) Antibodies
Epitope pSer1100
(26), (10), (9), (9), (8), (7), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(149), (22), (14), (4), (1)
Host Rabbit
(114), (32), (3), (1)
Clonality
Polyclonal
Conjugate This PIK3CG antibody is un-conjugated
(9), (9), (9), (9), (9), (9)
Application Enzyme Immunoassay (EIA)
(124), (80), (43), (23), (17), (16), (4), (3), (2), (2), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-PIK3CG Antibody

Target Details PIK3CG Application Details Handling ProductDetails: References for anti-PIK3CG antibody (ABIN358396) Images
Specificity This antibody detects PIK3CG pSer1100.
Purification Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1100 of human PIK3CG.
Isotype Ig

Target Details PIK3CG

Product Details anti-PIK3CG Antibody Application Details Handling ProductDetails: References for anti-PIK3CG antibody (ABIN358396) Images back to top
Antigen
Alternative Name PIK3CG (PIK3CG Antibody Abstract)
Background PIK3CG belongs to the pi3/pi4-kinase family of proteins. It is an enzyme that phosphorylates phosphoinositides on the 3-hydroxyl group of the inositol ring. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. The gene for this protein is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias.Synonyms: 5-bisphosphate 3-kinase catalytic subunit gamma isoform, PI3-kinase subunit gamma, PI3K-gamma, Phosphatidylinositol-4, PtdIns-3-kinase subunit p110-gamma, p120-PI3K
Molecular Weight 126454 Da
Gene ID 5294, 9606
UniProt P48736
Research Area Kinases/Phosphatases, Signaling, Cancer
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, TLR Signaling, Inositol Metabolic Process, Hepatitis C, VEGF Signaling

Application Details

Product Details anti-PIK3CG Antibody Target Details PIK3CG Handling ProductDetails: References for anti-PIK3CG antibody (ABIN358396) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-PIK3CG Antibody Target Details PIK3CG Application Details ProductDetails: References for anti-PIK3CG antibody (ABIN358396) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

ProductDetails: References for anti-PIK3CG antibody (ABIN358396)

Product Details anti-PIK3CG Antibody Target Details PIK3CG Application Details Handling Images back to top
Background publications

Reddy, Lin, Huang, Samanta, Liao: "The IL-1 receptor accessory protein is essential for PI 3-kinase recruitment and activation." in: Biochemical and biophysical research communications, Vol. 316, Issue 4, pp. 1022-8, 2004

Arai, Chikaraishi, Omura, Kuwajima: "First total synthesis of the antitumor compound (-)-kazusamycin A and absolute structure determination." in: Organic letters, Vol. 6, Issue 17, pp. 2845-8, 2004

Osaki, Kase, Adachi, Takeda, Hashimoto, Ito: "Inhibition of the PI3K-Akt signaling pathway enhances the sensitivity of Fas-mediated apoptosis in human gastric carcinoma cell line, MKN-45." in: Journal of cancer research and clinical oncology, Vol. 130, Issue 1, pp. 8-14, 2003

Théberge, Mehdi, Pandey, Srivastava: "Prolongation of insulin-induced activation of mitogen-activated protein kinases ERK 1/2 and phosphatidylinositol 3-kinase by vanadyl sulfate, a protein tyrosine phosphatase inhibitor." in: Archives of biochemistry and biophysics, Vol. 420, Issue 1, pp. 9-17, 2003

Khan, Di Cello, Nath, Kim: "Human immunodeficiency virus type 1 tat-mediated cytotoxicity of human brain microvascular endothelial cells." in: Journal of neurovirology, Vol. 9, Issue 6, pp. 584-93, 2003

Images

Product Details anti-PIK3CG Antibody Target Details PIK3CG Application Details Handling ProductDetails: References for anti-PIK3CG antibody (ABIN358396) back to top
Supplier Images
 image for anti-Phosphoinositide-3-Kinase, Catalytic, gamma Polypeptide (PIK3CG) (pSer1100) antibody (ABIN358396) anti-Phosphoinositide-3-Kinase, Catalytic, gamma Polypeptide (PIK3CG) (pSer1100) antibody