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anti-Chicken Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme antibody for Immunoprecipitation

Recommended Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme Antibody (supplied by: Log in to see )

Antigen
Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) Antibodies
  • PP2A
  • Pp2a1
  • pp2ac
  • PP2Ac
  • PP2CA
  • PP2Calpha
  • Ppp2ca
  • PPP2CA
  • R75353
  • RP-C
  • zgc:110641
Epitope
Catalytic Subunit
22
17
13
11
9
8
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
102
69
52
11
6
6
4
3
2
1
Host
Rabbit
99
11
4
3
3
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
98
42
24
23
14
11
10
5
2
2
Supplier
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Catalog No. ABIN493370
$ 456.50
Plus shipping costs $45.00

Top referenced anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme antibody for Immunoprecipitation

Similar anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken
Western Blotting (WB) 87 Antibodies 59 Antibodies 42 Antibodies 3 Antibodies
Immunoprecipitation (IP) 14 Antibodies 7 Antibodies 6 Antibodies 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 27 Antibodies 16 Antibodies 16 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies 11 Antibodies
Immunofluorescence (IF) 17 Antibodies 14 Antibodies

General

Antigen Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) Antibodies
Epitope Catalytic Subunit
(22), (17), (13), (11), (9), (8), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Human, Mouse (Murine), Rat (Rattus)
(102), (69), (52), (11), (6), (6), (4), (3), (2), (1)
Host Rabbit
(99), (11), (4), (3), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(98), (42), (24), (23), (14), (11), (10), (5), (2), (2)
Pubmed 4 references available
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Product details

Target details Application Details Handling References Images
Specificity This antibody detects a single band of approximately 36 kDa of PP2A in Western Blot positive control. It does not cross reacts with other proteins from the protein kinase family or with other proteins.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse, Rat, Chicken and Monkey.
Purification Affinity Chromatography over immobilized antigen
Immunogen The PP2Ac-selective antibodies were generated against purified human kidney protein Phosphatase 2A catalytic subunit.
Isotype IgG

Target details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name PPP2CA (PPP2CA Antibody Abstract)
Background PP1 and PP2A are members of the protein serine/threonine phosphatases (PPs) family and their activities have been implicated in many cellular signal transduction processes including the steroid synthesis regulation via hormonally regulated cAMP, regulation of insulin dependent glucose uptake and glycogen synthesis, apoptotic activity in chronic lymphocytic leukemia (CLL) via de-phosphorylation of pro-apoptotic BH3-onlyBcl-2 family members etc. (1, 2). The protein phosphatase 2A (PP2A) holoenzyme is composed of one catalytic C subunit, one regulatory/scaffolding A subunit, and one regulatory B subunit. The native core enzyme consists of A and C subunits. The A and C subunits are found in two closely related isoforms, alpha and beta. The regulatory B subunits belongs to four distinct/weakly related families designated as B, B', B", and B"'. The B family has multiple variants. The existence of two A and two C subunit isoforms allows the PP2A complex to be expressed in four core enzymes (Aa/Ca, Aa/Cb, Ab/Ca, Ab/Cb). Enzymatically, various haloenzymes are identical but their expression and subcellular localization during early embryonic development have been distinct suggesting they might have different functions (3). Understanding the mechanisms that regulate cell migration is important for developing novel therapeutic drugs for the treatment of metastasis and for enhanced wound healing. Inhibitors of PP2A slowed down the de-phosphorylation of extracellular-signal related kinase (ERK) in keratinocytes in response to beta adrenergic receptor activation, which alters the migration rate of these cells. The PP2A is also an excellent therapeutic target for development of novel anticancer agents (Cantharimides) such as, norcantharidin, which exhibited a broad spectrum of anti-cancer activity (4) and for enhanced wound healing by altering cellular migration.Synonyms: PP2A-alpha, Protein Phospatase 2A, Replication protein C, Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
Gene ID 5515
NCBI Accession NP_002706
UniProt P67775
Research Area Kinases/Phosphatases, Signaling, Protein Modifications
Pathways PI3K-Akt Signaling

Application Details

Product details Target details Handling References Images back to top
Application Notes This antibody is ideal for Immunoprecipitation and Western Blot. Recommended Dilution: Western Blot: > 1/250
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product details Target details Application Details References Images back to top
Concentration 1-1.25 mg/mL
Buffer Stabilization buffer with 0.02 % Thimerosal (merthiolate) as preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at-20 °C.

References

Product details Target details Application Details Handling Images back to top
Background publications

Zhou, Pham, Walter: "The formation and activity of PP2A holoenzymes do not depend on the isoform of the catalytic subunit." in: The Journal of biological chemistry, Vol. 278, Issue 10, pp. 8617-22, 2003

Moon, Lerner: "PDE4 inhibitors activate a mitochondrial apoptotic pathway in chronic lymphocytic leukemia cells that is regulated by protein phosphatase 2A." in: Blood, Vol. 101, Issue 10, pp. 4122-30, 2003

McCluskey, Ackland, Gardiner, Walkom, Sakoff: "The inhibition of protein phosphatases 1 and 2A: a new target for rational anti-cancer drug design?" in: Anti-cancer drug design, Vol. 16, Issue 6, pp. 291-303, 2002

ABEGG: "[Not Available]." in: Ciba-Zeitschrift, Vol. 10, Issue 112, pp. 4122-8, 1948

Images

Product details Target details Application Details Handling References back to top
Supplier Images
 image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (Catalytic Subunit) antibody (ABIN493370) anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (Catalytic Subunit) antibody