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anti-Human Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme antibody for Immunocytochemistry

Recommended Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme Antibody (supplied by: Log in to see )

Antigen
Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) Antibodies
  • PP2A
  • Pp2a1
  • pp2ac
  • PP2Ac
  • PP2CA
  • PP2Calpha
  • Ppp2ca
  • PPP2CA
  • R75353
  • RP-C
  • zgc:110641
Epitope
C-Term
23
17
13
11
9
8
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
107
73
54
11
6
6
5
3
2
1
1
1
Host
Rabbit
101
15
4
3
3
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
103
44
26
25
14
11
11
5
2
2
Supplier
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Catalog No. ABIN2856333
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009965416 ABIN258417 ICC IF IHC (p) IP IHC WB Rabbit IgG Tyr307 Log in to see E155
0.0009965416 ABIN600145 ICC IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
0.0009965416 ABIN1495037 ICC IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.0009965416 ABIN2884262 ICC IHC ELISA WB Rabbit IgG pTyr307 Log in to see Polyclonal
0.0009965416 ABIN408077 ICC IF IHC (p) IHC WB Rabbit IgG Thr301 Log in to see Polyclonal
0.0009965416 ABIN441297 ICC IF IHC IHC (p) IP WB Rabbit IgG Log in to see Polyclonal
0.0009965416 ABIN4346918 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
0.0009965416 ABIN2969825 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal

Similar anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 13 Antibodies 27 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 6 Antibodies
Immunofluorescence (IF) 13 Antibodies 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 17 Antibodies 18 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 14 Antibodies 16 Antibodies
Immunoprecipitation (IP) 6 Antibodies 8 Antibodies
Western Blotting (WB) 44 Antibodies

General

Antigen Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) Antibodies
Epitope C-Term
(23), (17), (13), (11), (9), (8), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(107), (73), (54), (11), (6), (6), (5), (3), (2), (1), (1), (1)
Host Rabbit
(101), (15), (4), (3), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(103), (44), (26), (25), (14), (11), (11), (5), (2), (2)
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Product details

Target details Application Details Handling Images
Cross-Reactivity Rat (Rattus), Zebrafish (Danio rerio), Xenopus laevis, Xenopus tropicalis, Dog (Canine), Pig (Porcine), Rabbit, Chicken, Cow (Bovine)
Cross-Reactivity (Details) Rat (100 %), Zebrafish (100 %), Xenopus laevis (100 %), Xenopus Tropicalis (100 %), Dog (100 %), Pig (100 %), Rabbit (100 %), Chicken (100 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to PP2A alpha (protein phosphatase 2, catalytic subunit, alpha isozyme)
PP2A alpha antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PP2A alpha. The exact sequence is proprietary.
Isotype IgG

Target details

Product details Application Details Handling Images back to top
Antigen
Alternative Name PP2A alpha (PPP2CA Antibody Abstract)
Background This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.

Cellular Localization: Cytoplasm , Nucleus , Centromere , Spindle
Molecular Weight 36 kDa
Gene ID 5515
Research Area Signaling, Kinases/Phosphatases
Pathways PI3K-Akt Signaling

Application Details

Product details Target details Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*
Comment

Positive Control: 293T , A431 , H1299 , HeLaS3 , Molt-4 , Raji , mouse brain

Restrictions For Research Use only

Handling

Product details Target details Application Details Images back to top
Format Liquid
Concentration 0.479 mg/mL
Buffer 1XPBS, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product details Target details Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (C-Term) antibody (ABIN2856333) ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde...
Immunoprecipitation (IP) image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (C-Term) antibody (ABIN2856333) IP Image Immunoprecipitation of PP2A alpha protein from 293T whole cell extracts usin...
Immunohistochemistry (IHC) image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (C-Term) antibody (ABIN2856333) IHC-P Image Immunohistochemical analysis of paraffin-embedded A549 xenograft, using P...
Western Blotting (WB) image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (C-Term) antibody (ABIN2856333) WB Image Sample (30 ug of whole cell lysate) A: 293T B: Molt-4 , 10% SDS PAGE antibod...
Western Blotting (WB) image for anti-Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme (PPP2CA) (C-Term) antibody (ABIN2856333) WB Image Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE antibody dil...