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anti-Human Protein Phosphatase 2, Regulatory Subunit B', gamma antibody for Immunoprecipitation

Recommended Protein Phosphatase 2, Regulatory Subunit B', gamma Antibody (supplied by: Log in to see )

Antigen
Protein Phosphatase 2, Regulatory Subunit B', gamma (PPP2CA) Antibodies
  • b56g
  • ppp2r5c
  • si:dkey-241l7.9
  • 2610043M05Rik
  • 2700063L20Rik
  • AI060890
  • AW545884
  • C85228
  • D12Bwg0916e
  • mKIAA0044
  • B56G
  • PR61G
  • protein phosphatase 2, regulatory subunit B', gamma
  • protein phosphatase 2, regulatory subunit B', gamma b
  • protein phosphatase 2, regulatory subunit B (B56), gamma isoform
  • ppp2r5c
  • PPP2R5C
  • ppp2r5cb
  • Ppp2r5c
Reactivity
Human, Mouse (Murine)
66
37
26
5
3
2
2
2
2
2
2
2
2
1
1
Host
Mouse
45
20
1
Clonality
Monoclonal
Conjugate
Un-conjugated
5
4
4
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Western Blotting (WB)
52
16
13
8
4
4
1
1
1
Supplier
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Available images

Catalog No. ABIN151944
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4347115 IP WB FITC Mouse IgG1 kappa Log in to see TQ11-1G6
1 ABIN1926019 IP Rabbit IgG AA 440-490 Log in to see Polyclonal
1 ABIN1926020 IP WB Rabbit IgG AA 490-524 Log in to see Polyclonal
1 ABIN1721953 IP WB Biotin Mouse IgG1 kappa Log in to see TQ11-1G6
1 ABIN1721955 IP WB DyLight 488 Mouse IgG1 kappa Log in to see TQ11-1G6
1 ABIN1721957 IP WB DyLight 550 Mouse IgG1 kappa Log in to see TQ11-1G6
1 ABIN1721954 IP WB DyLight 650 Mouse IgG1 kappa Log in to see TQ11-1G6
1 ABIN1721956 IP WB HRP Mouse IgG1 kappa Log in to see TQ11-1G6

Top referenced anti-Protein Phosphatase 2, Regulatory Subunit B', gamma antibody for Immunoprecipitation

Similar anti-Protein Phosphatase 2, Regulatory Subunit B', gamma Antibodies

Application / Reactivity Mouse (Murine) Human
Immunocytochemistry (ICC) 1 Antibodies 1 Antibodies
Immunofluorescence (IF) 1 Antibodies 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 4 Antibodies 4 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 4 Antibodies 4 Antibodies
Immunoprecipitation (IP) 2 Antibodies 9 Antibodies
Western Blotting (WB) 26 Antibodies 53 Antibodies
ELISA 16 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen Protein Phosphatase 2, Regulatory Subunit B', gamma (PPP2CA) Antibodies
Reactivity Human, Mouse (Murine)
(66), (37), (26), (5), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(45), (20), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Western Blotting (WB)
(52), (16), (13), (8), (4), (4), (1), (1), (1)
Pubmed 3 references available
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Product details

Target details Application Details Handling References Images
Specificity This is specific for PP2A (B56 gamma 3 subunit) protein. This does not recognize the B56 gamma 1 isoform of PP2A.
Purification Protein G purified
Immunogen Human PPP2R5C [UniProt# Q13362]
Clone TQ11-1G6
Isotype IgG1 kappa

Target details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name PPP2R5C (PPP2CA Antibody Abstract)
Background Gene Symbol: PPP2R5C
Gene ID 5527
UniProt Q13362
Research Area Signaling
Pathways PI3K-Akt Signaling

Application Details

Product details Target details Handling References Images back to top
Application Notes Western Blot 1:1000, Immunoprecipitation 1:10-1:500This PPP2R5C (TQ11-1G6) antibody is useful for Immunoprecipitation and Western blot. By Western blot, this antibody recognizes overexpressed PPP2R5C protein in transfected cells. Bands at ~50 and 35 kDa may also be seen. Western Blot analysis of endogenous samples is not recommended due to high background. Has been shown to work effectively for Immunoprecipitation, see Lin et al.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product details Target details Application Details References Images back to top
Format Liquid
Concentration 2 mg/mL
Buffer PBS
Buffer contains: 0.1 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

References

Product details Target details Application Details Handling Images back to top
Product cited in:

Kuo, Huang, Yang, Yang, Lee, Chiang: "Regulation of phosphorylation of Thr-308 of Akt, cell proliferation, and survival by the B55alpha regulatory subunit targeting of the protein phosphatase 2A holoenzyme to Akt." in: The Journal of biological chemistry, Vol. 283, Issue 4, pp. 1882-92, 2008 (Sample species: Mouse (Murine)). Further details: Western Blotting

Lin, Bassik, Suh, Nishino, Arroyo, Hahn, Korsmeyer, Roberts: "PP2A regulates BCL-2 phosphorylation and proteasome-mediated degradation at the endoplasmic reticulum." in: The Journal of biological chemistry, Vol. 281, Issue 32, pp. 23003-12, 2006 (Sample species: Mouse (Murine)). Further details: Immunoprecipitation

Kumamoto: "A contact-activated kinase signals Candida albicans invasive growth and biofilm development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 15, pp. 5576-81, 2005

Images

Product details Target details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-Protein Phosphatase 2, Regulatory Subunit B', gamma (PPP2CA) antibody (ABIN151944) Detection of PP2A using ABIN151944.
Western Blotting (WB) image for anti-Protein Phosphatase 2, Regulatory Subunit B', gamma (PPP2CA) antibody (ABIN151944) Western Blot: PPP2R5C Antibody (TQ11-1G6) [ABIN151944] - Analysis of PPP2R5C B56 gamm...