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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||2 Antibodies|
|Immunocytochemistry (ICC)||3 Antibodies|
|Immunofluorescence (IF)||3 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||24 Antibodies|
|Immunohistochemistry (IHC)||13 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||17 Antibodies|
|Western Blotting (WB)||26 Antibodies|
|Antigen||Protein Phosphatase 2, Regulatory Subunit B'', alpha (PPP2R3A) Antibodies|
|Epitope||Internal Region Alternatives|
|Conjugate||This PPP2R3A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-PPP2R3A AntibodyTarget Details PPP2R3A Application Details Handling Images
|Specificity||This antibody reacts to Protein Phosphatase Pp2A, 130 Kd Regulatory Subunit (PPP2R3A).|
|Immunogen||Synthetic peptide (C-QKDVENDGPEPSD) from an internal region of Human PPP2R3A|
Target Details PPP2R3AProduct Details anti-PPP2R3A Antibody Application Details Handling Images back to top
|Alternative Name||PPP2R3A / PPP2R3 (PPP2R3A Antibody Abstract)|
|Background||Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.Synonyms: B72/B130 isoforms, PP2A subunit B B''-PR72/PR130, PP2A subunit B PR72/PR130 isoforms, Serine/threonine-protein phosphatase 2A 72/130 kDa regulatory subunit B, Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha, phosphatase 2A regulatory subunit B'' alpha|
Application DetailsProduct Details anti-PPP2R3A Antibody Target Details PPP2R3A Handling Images back to top
ELISA: 1/16000. Western blot: 1 μg/mL. Immunohistochemistry on Paraffin Sections: 5 μg/mL. This PPP2R3A antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PPP2R3A Antibody Target Details PPP2R3A Application Details Images back to top
|Buffer||Tris Saline buffer, pH 7.3 containing 0.5 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage Comment||Store the antibody (in aliquots) at-20 °C.|
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