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anti-Human PPP2R4 antibody for Immunohistochemistry

Recommended PPP2R4 Antibody (supplied by: Log in to see )

Antigen
Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) Antibodies
  • 2610042B21Rik
  • 6330556D22Rik
  • C77440
  • N28142
  • PP2A
  • PR53
  • PR65
  • PTPA
  • PTPRA
Reactivity
Human
59
29
26
4
4
2
1
1
1
1
1
Host
Mouse
41
13
10
Clonality
Monoclonal
Conjugate
This PPP2R4 antibody is un-conjugated
2
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
58
21
20
15
10
9
5
3
2
2
1
1
Supplier
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Catalog No. ABIN969557
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22919032 ABIN1874229 IHC WB Rabbit IgG Log in to see Polyclonal
0.22919032 ABIN2404794 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.22919032 ABIN1844821 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see
0.22919032 ABIN3015701 IHC WB Rabbit Log in to see Polyclonal
0.0011344256 ABIN1500384 ICC FACS IF IHC ELISA WB Mouse IgG1 Log in to see 4D9
0.0011344256 ABIN1585083 ICC IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
0.0011344256 ABIN3198271 IC IF IHC WB Rabbit Log in to see Polyclonal
0.0011344256 ABIN1585080 IHC ELISA WB Mouse C-Term Log in to see Polyclonal
0.0011344256 ABIN1585081 IHC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0011344256 ABIN2339929 FACS IHC ELISA WB Mouse IgG1 Log in to see 13B638
0.0011344256 ABIN2339218 IHC WB Rabbit IgG N-Term Log in to see Polyclonal
0.0011344256 ABIN2700009 IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0011344256 ABIN4347104 ELISA FACS ICC IF IHC WB Mouse IgG1 Log in to see 4D9
0.0011344256 ABIN4347105 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0011344256 ABIN2972994 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0011344256 ABIN2149480 IHC ELISA WB HRP Rabbit IgG Catalytic Subunit beta Log in to see Polyclonal
0.0011344256 ABIN2149481 IHC ELISA WB HRP Rabbit IgG Catalytic Subunit beta Log in to see Polyclonal
0.0011344256 ABIN2149483 IHC ELISA WB Rabbit IgG Catalytic Subunit beta Log in to see Polyclonal
0.0011344256 ABIN1855187 IHC ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-PPP2R4 antibody for Immunohistochemistry

Similar anti-PPP2R4 Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 2 Antibodies
ELISA 21 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 6 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunohistochemistry (IHC) 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 8 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 55 Antibodies

General

Antigen Protein Phosphatase 2A Activator, Regulatory Subunit 4 (PPP2R4) Antibodies
Reactivity Human
(59), (29), (26), (4), (4), (2), (1), (1), (1), (1), (1)
Host Mouse
(41), (13), (10)
Clonality (Clone)
Monoclonal   ( )
Conjugate This PPP2R4 antibody is un-conjugated
(2), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(58), (21), (20), (15), (10), (9), (5), (3), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-PPP2R4 Antibody

Target Details PPP2R4 Application Details Handling References for anti-PPP2R4 Antibody (ABIN969557) Images
Purification Purified
Immunogen Purified recombinant fragment of human PPP2R4 expressed in E. coli.
Clone 4D9
Isotype IgG1

Target Details PPP2R4

Product Details anti-PPP2R4 Antibody Application Details Handling References for anti-PPP2R4 Antibody (ABIN969557) Images back to top
Antigen
Alternative Name PPP2R4 (PPP2R4 Antibody Abstract)
Background Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.
Molecular Weight 41 kDa
Gene ID 5524
HGNC 5524
Research Area Signaling
Pathways PI3K-Akt Signaling

Application Details

Product Details anti-PPP2R4 Antibody Target Details PPP2R4 Handling References for anti-PPP2R4 Antibody (ABIN969557) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-PPP2R4 Antibody Target Details PPP2R4 Application Details References for anti-PPP2R4 Antibody (ABIN969557) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-PPP2R4 Antibody (ABIN969557)

Product Details anti-PPP2R4 Antibody Target Details PPP2R4 Application Details Handling Images back to top
Product cited in:

Yoon, Jun, Park, Jun Yoo, Moon, Soon Baik, Kim, Kim, Kim, Koh, Taek Lee, You: "Optimal suppression of protein phosphatase 2A activity is critical for maintenance of human embryonic stem cell self-renewal." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 5, pp. 874-84, 2010

Singh, Ye, Bucur, Zhu, Tanya Santos, Rabinovitz, Wei, Gao, Hahn, Khosravi-Far: "Protein phosphatase 2A reactivates FOXO3a through a dynamic interplay with 14-3-3 and AKT." in: Molecular biology of the cell, Vol. 21, Issue 6, pp. 1140-52, 2010

Images

Product Details anti-PPP2R4 Antibody Target Details PPP2R4 Application Details Handling References for anti-PPP2R4 Antibody (ABIN969557) back to top
Supplier Images
ELISA image for anti-PPP2R4 antibody (Protein Phosphatase 2A Activator, Regulatory Subunit 4) (ABIN969557) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-PPP2R4 antibody (Protein Phosphatase 2A Activator, Regulatory Subunit 4) (ABIN969557) Figure 2: Flow cytometric analysis of MCF-7 cells using PPP2R4 mouse mAb (green) and ...
Immunocytochemistry (ICC) image for anti-PPP2R4 antibody (Protein Phosphatase 2A Activator, Regulatory Subunit 4) (ABIN969557) Figure 3: Immunofluorescence analysis of Hela cells using PPP2R4 mouse mAb (green) . ...
Immunohistochemistry (IHC) image for anti-PPP2R4 antibody (Protein Phosphatase 2A Activator, Regulatory Subunit 4) (ABIN969557) Figure 4: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues u...
Immunohistochemistry (IHC) image for anti-PPP2R4 antibody (Protein Phosphatase 2A Activator, Regulatory Subunit 4) (ABIN969557) Figure 5: Immunohistochemical analysis of paraffin-embedded lung cancer tissues using...
Western Blotting (WB) image for anti-PPP2R4 antibody (Protein Phosphatase 2A Activator, Regulatory Subunit 4) (ABIN969557) Figure 6: Western blot analysis using PPP2R4 mAb against human PPP2R4 (AA: 1-154) rec...