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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Cow (Bovine)|
|Immunofluorescence (fixed cells) (IF/ICC)||17 Antibodies||15 Antibodies||15 Antibodies||9 Antibodies|
|Simple Western (SimWes)||1 Antibodies||1 Antibodies||1 Antibodies|
|Western Blotting (WB)||313 Antibodies||292 Antibodies||69 Antibodies||522 Antibodies|
|Radioimmunoassay (RIA)||1 Antibodies||1 Antibodies||1 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Immunoprecipitation (IP)||64 Antibodies||43 Antibodies|
|Antigen||Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies|
|Reactivity||Cow (Bovine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This RPS6KB1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
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Product Details anti-RPS6KB1 AntibodyTarget Details RPS6KB1 Application Details Handling Images
|Specificity||This antibody detects a ~70 kDa protein, corresponding to the molecular mass of p70 S6K on SDS-PAGE immunoblots.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Mouse, Rat, Bovine|
|Immunogen||Human p70 S6K C-terminal peptide - KLH conjugates|
Target Details RPS6KB1Product Details anti-RPS6KB1 Antibody Application Details Handling Images back to top
|Alternative Name||RPS6KB1 / STK14A (RPS6KB1 Antibody Abstract)|
|Background||Ribosomal S6 (S6K) belongs to the AGC family of Serine/Threonine protein kinases. There are two forms of S6K, alpha and beta, which have cytoplasmic and nuclear variants derived from alternative splicing (1). The activity of S6K is regulated by phosphorylation/dephosphorylation events in cellular responses to various extracellular stimuli. Its activity has also been shown to be dependent on nutrient availability (3-6). The mechanism of activation of S6K is a multi-step process which is achieved by phosphorylating the 40S ribosomal protein S6, which then upregulates the translation of mRNA transcripts containing an oligopryimidine tract at the 5' transcriptional start site (3-6). Recently, a highly homologous ribosomal S6 kinase, termed S6K2, was identified. It has 70 % amino acid identity in the overall sequence with S6K1, and highly conserved potential phosphorylation sites of S6K1. However, the N- and Cterminal domains of S6K2 are quite different (2). As a result, S6K is a key player in the regulation of cell size, growth and glucose homeostasis.Synonyms: 70 kDa ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase I, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, Serine/threonine-protein kinase 14A, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, p70-S6K 1|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma|
Application DetailsProduct Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Handling Images back to top
Western blot (1): 0.25 μg/mL. Immunoprecipitation (1). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Images back to top
|Buffer||TBS, 0.09 % Sodium Azide, 50 % Glycerol|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|