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anti-Cow (Bovine) RPS6KB1 antibody for Immunoprecipitation

Recommended RPS6KB1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
  • 70kDa
  • 2610318I15Rik
  • 4732464A07Rik
  • AA959758
  • AI256796
  • AI314060
  • p70 S6KA
  • p70(S6K)-alpha
  • p70-alpha
  • p70-S6K
  • p70/85s6k
  • p70S6K
  • p70s6k
  • PS6K
  • S6K
  • S6K-beta-1
  • S6K1
  • STK14A
Epitope
C-Term
108
35
31
29
24
24
21
19
18
18
18
17
16
15
14
13
13
13
13
11
9
8
8
7
6
5
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
649
359
344
47
30
21
16
15
6
1
1
1
1
Host
Rabbit
591
40
25
2
Clonality
Polyclonal
Conjugate
This RPS6KB1 antibody is un-conjugated
24
22
21
13
13
13
8
8
8
8
8
8
8
8
8
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
497
283
251
148
93
88
58
50
19
11
10
8
1
1
1
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Catalog No. ABIN264871
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089556724 ABIN361700 ICC IF IP ELISA WB Rabbit C-Term Log in to see Polyclonal 6
0.00089556724 ABIN2486867 ICC IF IP ELISA WB Atto 390 Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2486868 ICC IF IP ELISA WB Atto 488 Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2486869 ICC IF IP ELISA WB Atto 565 Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2486870 ICC IF IP ELISA WB Atto 594 Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2486871 ICC IF IP ELISA WB Atto 633 Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2486872 ICC IF IP ELISA WB Atto 655 Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2486874 ICC IF IP ELISA WB Atto 700 Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2486873 ICC IF IP ELISA WB Atto 680 Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2486875 ICC IF IP ELISA WB Alkaline Phosphatase (AP) Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2486876 ICC IF IP ELISA WB APC Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2486877 ICC IF IP ELISA WB Biotin Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2486878 ICC IF IP ELISA WB FITC Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2486880 ICC IF IP ELISA WB PE-Atto 594 Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2486879 ICC IF IP ELISA WB HRP Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2486881 ICC IF IP ELISA WB PerCP Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2486882 ICC IF IP ELISA WB PE Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2486883 ICC IF IP ELISA WB Streptavidin Rabbit C-Term Log in to see Polyclonal 3
0.00089556724 ABIN2705216 IP IHC WB Rabbit C-Term Log in to see Polyclonal
0.00089556724 ABIN2705219 IC IF IP IHC WB Rabbit C-Term, pSer434 Log in to see Polyclonal

Top referenced anti-RPS6KB1 antibody for Immunoprecipitation

Similar anti-RPS6KB1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine)
Western Blotting (WB) 490 Antibodies 253 Antibodies 241 Antibodies 43 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Immunoprecipitation (IP) 57 Antibodies 32 Antibodies 35 Antibodies 22 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 93 Antibodies 52 Antibodies 49 Antibodies 2 Antibodies
Immunohistochemistry (IHC) 279 Antibodies 160 Antibodies 153 Antibodies 21 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
Epitope C-Term
(108), (35), (31), (29), (24), (24), (21), (19), (18), (18), (18), (17), (16), (15), (14), (13), (13), (13), (13), (11), (9), (8), (8), (7), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(649), (359), (344), (47), (30), (21), (16), (15), (6), (1), (1), (1), (1)
Host Rabbit
(591), (40), (25), (2)
Clonality
Polyclonal
Conjugate This RPS6KB1 antibody is un-conjugated
(24), (22), (21), (13), (13), (13), (8), (8), (8), (8), (8), (8), (8), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
(497), (283), (251), (148), (93), (88), (58), (50), (19), (11), (10), (8), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-RPS6KB1 Antibody

Target Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 Antibody (ABIN264871) Images
Specificity This antibody detects a ~70 kDa protein, corresponding to the molecular mass of p70 S6K on SDS-PAGE immunoblots.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse, Rat, Bovine
Purification Affinity Chromatography
Immunogen Human p70 S6K C-terminal peptide - KLH conjugates
Isotype IgG

Target Details RPS6KB1

Product Details anti-RPS6KB1 Antibody Application Details Handling References for anti-RPS6KB1 Antibody (ABIN264871) Images back to top
Antigen
Alternative Name RPS6KB1 / STK14A (RPS6KB1 Antibody Abstract)
Background Ribosomal S6 (S6K) belongs to the AGC family of Serine/Threonine protein kinases. There are two forms of S6K, alpha and beta, which have cytoplasmic and nuclear variants derived from alternative splicing (1). The activity of S6K is regulated by phosphorylation/dephosphorylation events in cellular responses to various extracellular stimuli. Its activity has also been shown to be dependent on nutrient availability (3-6). The mechanism of activation of S6K is a multi-step process which is achieved by phosphorylating the 40S ribosomal protein S6, which then upregulates the translation of mRNA transcripts containing an oligopryimidine tract at the 5' transcriptional start site (3-6). Recently, a highly homologous ribosomal S6 kinase, termed S6K2, was identified. It has 70 % amino acid identity in the overall sequence with S6K1, and highly conserved potential phosphorylation sites of S6K1. However, the N- and Cterminal domains of S6K2 are quite different (2). As a result, S6K is a key player in the regulation of cell size, growth and glucose homeostasis.Synonyms: 70 kDa ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase I, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, Serine/threonine-protein kinase 14A, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, p70-S6K 1
Gene ID 6198
NCBI Accession NP_003152
UniProt P23443
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Handling References for anti-RPS6KB1 Antibody (ABIN264871) Images back to top
Application Notes Western blot (1): 0.25 μg/mL. Immunoprecipitation (1). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details References for anti-RPS6KB1 Antibody (ABIN264871) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer TBS, 0.09 % Sodium Azide, 50 % Glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-RPS6KB1 Antibody (ABIN264871)

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling Images back to top
Background publications

Valovka, Filonenko, Velykyï, Drobot, Voterfill, Matsuka, Hut: "[Features of fibronectin-dependent activation of ribosomal protein S6 kinase (S6K1 and S6K2)]." in: Ukrai?ns?kyi? biokhimichnyi? z?h?urnal (1999 ), Vol. 72, Issue 3, pp. 31-7, 2001

Shima, Pende, Chen, Fumagalli, Thomas, Kozma: "Disruption of the p70(s6k)/p85(s6k) gene reveals a small mouse phenotype and a new functional S6 kinase." in: The EMBO journal, Vol. 17, Issue 22, pp. 6649-59, 1999

Lee-Fruman, Kuo, Lippincott, Terada, Blenis: "Characterization of S6K2, a novel kinase homologous to S6K1." in: Oncogene, Vol. 18, Issue 36, pp. 5108-14, 1999

Gout, Minami, Hara, Tsujishita, Filonenko, Waterfield, Yonezawa: "Molecular cloning and characterization of a novel p70 S6 kinase, p70 S6 kinase beta containing a proline-rich region." in: The Journal of biological chemistry, Vol. 273, Issue 46, pp. 30061-4, 1998

Images

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 Antibody (ABIN264871) back to top
Supplier Images
 image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (C-Term) (ABIN264871) anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (C-Term) antibody