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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Cow (Bovine)|
|Western Blotting (WB)||487 Antibodies||253 Antibodies||242 Antibodies||43 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Immunoprecipitation (IP)||57 Antibodies||32 Antibodies||35 Antibodies||22 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||93 Antibodies||52 Antibodies||49 Antibodies||2 Antibodies|
|Immunohistochemistry (IHC)||274 Antibodies||160 Antibodies||153 Antibodies||21 Antibodies|
|Antigen||Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies|
|Reactivity||Cow (Bovine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This RPS6KB1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-RPS6KB1 AntibodyTarget Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 Antibody (ABIN264871) Images
|Specificity||This antibody detects a ~70 kDa protein, corresponding to the molecular mass of p70 S6K on SDS-PAGE immunoblots.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Mouse, Rat, Bovine|
|Immunogen||Human p70 S6K C-terminal peptide - KLH conjugates|
Target Details RPS6KB1Product Details anti-RPS6KB1 Antibody Application Details Handling References for anti-RPS6KB1 Antibody (ABIN264871) Images back to top
|Alternative Name||RPS6KB1 / STK14A (RPS6KB1 Antibody Abstract)|
|Background||Ribosomal S6 (S6K) belongs to the AGC family of Serine/Threonine protein kinases. There are two forms of S6K, alpha and beta, which have cytoplasmic and nuclear variants derived from alternative splicing (1). The activity of S6K is regulated by phosphorylation/dephosphorylation events in cellular responses to various extracellular stimuli. Its activity has also been shown to be dependent on nutrient availability (3-6). The mechanism of activation of S6K is a multi-step process which is achieved by phosphorylating the 40S ribosomal protein S6, which then upregulates the translation of mRNA transcripts containing an oligopryimidine tract at the 5' transcriptional start site (3-6). Recently, a highly homologous ribosomal S6 kinase, termed S6K2, was identified. It has 70 % amino acid identity in the overall sequence with S6K1, and highly conserved potential phosphorylation sites of S6K1. However, the N- and Cterminal domains of S6K2 are quite different (2). As a result, S6K is a key player in the regulation of cell size, growth and glucose homeostasis.Synonyms: 70 kDa ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase I, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, Serine/threonine-protein kinase 14A, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, p70-S6K 1|
|Pathways||PI3K-Akt Signaling, RTK Signaling, AMPK Signaling|
Application DetailsProduct Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Handling References for anti-RPS6KB1 Antibody (ABIN264871) Images back to top
Western blot (1): 0.25 μg/mL. Immunoprecipitation (1). ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details References for anti-RPS6KB1 Antibody (ABIN264871) Images back to top
|Buffer||TBS, 0.09 % Sodium Azide, 50 % Glycerol|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-RPS6KB1 Antibody (ABIN264871)Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling Images back to top
Valovka, Filonenko, Velykyï et al.: "[Features of fibronectin-dependent activation of ribosomal protein S6 kinase (S6K1 and S6K2)]." in: Ukrai?ns?kyi? biokhimichnyi? z?h?urnal (1999 ), Vol. 72, Issue 3, pp. 31-7, 2001 (PubMed).
Lee-Fruman, Kuo, Lippincott et al.: "Characterization of S6K2, a novel kinase homologous to S6K1." in: Oncogene, Vol. 18, Issue 36, pp. 5108-14, 1999 (PubMed).
Shima, Pende, Chen et al.: "Disruption of the p70(s6k)/p85(s6k) gene reveals a small mouse phenotype and a new functional S6 kinase." in: The EMBO journal, Vol. 17, Issue 22, pp. 6649-59, 1999 (PubMed).
Gout, Minami, Hara et al.: "Molecular cloning and characterization of a novel p70 S6 kinase, p70 S6 kinase beta containing a proline-rich region." in: The Journal of biological chemistry, Vol. 273, Issue 46, pp. 30061-4, 1998 (PubMed).
ImagesProduct Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 Antibody (ABIN264871) back to top