You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human RPS6KB1 antibody for Flow Cytometry

Recommended RPS6KB1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
  • 70kDa
  • 2610318I15Rik
  • 4732464A07Rik
  • AA959758
  • AI256796
  • AI314060
  • p70 S6KA
  • p70(S6K)-alpha
  • p70-alpha
  • p70-S6K
  • p70/85s6k
  • p70S6K
  • p70s6k
  • PS6K
  • S6K
  • S6K-beta-1
  • S6K1
  • STK14A
Reactivity
Human
653
365
350
48
30
21
16
15
6
1
1
1
1
Host
Mouse
592
44
25
2
Clonality
Monoclonal
Conjugate
This RPS6KB1 antibody is un-conjugated
24
22
21
14
13
13
9
8
8
8
8
8
8
8
8
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
499
286
251
148
93
88
59
50
19
14
11
9
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN969565
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.29105452 ABIN1108904 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 5G9 2
0.29105452 ABIN2454595 FACS IF WB Mouse IgG1 Log in to see 4G4
0.29105452 ABIN1846066 FACS IHC ELISA WB Mouse IgG1 Log in to see
0.0010392143 ABIN2454579 FACS IF IHC WB Mouse IgG1 Log in to see 1G4
0.0010392143 ABIN1500000 FACS IHC ELISA WB Mouse IgG1 Log in to see 5G9
0.0010392143 ABIN4881031 ELISA FACS IHC WB Mouse IgG1 Log in to see 5G9
0.0010392143 ABIN2627749 FACS Mouse IgG1 Log in to see 1G4
0.0010392143 ABIN2627750 FACS Mouse IgG1 Log in to see 4G4
0.0010392143 ABIN2354192 FACS IHC ELISA WB Mouse IgG1 Log in to see 13B683
0.0010392143 ABIN4898816 FACS Alexa Fluor 488 Mouse IgG1 Log in to see 215247
0.0010392143 ABIN4898817 FACS PE Mouse IgG1 Log in to see 215247
0.0010392143 ABIN4900839 FACS IHC WB Mouse IgG1 Log in to see 215247
0.0010392143 ABIN4900838 FACS IHC SimWes WB Rabbit IgG Log in to see Polyclonal 1
0.0010392143 ABIN3069449 ICC FACS IF IP IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-RPS6KB1 antibody for Flow Cytometry

Similar anti-RPS6KB1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 19 Antibodies
ELISA 250 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 15 Antibodies
Immunochromatography (IC) 11 Antibodies
Immunocytochemistry (ICC) 50 Antibodies
Immunofluorescence (IF) 148 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 88 Antibodies
Immunohistochemistry (IHC) 282 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 93 Antibodies
Immunoprecipitation (IP) 56 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 490 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
Reactivity Human
(653), (365), (350), (48), (30), (21), (16), (15), (6), (1), (1), (1), (1)
Host Mouse
(592), (44), (25), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This RPS6KB1 antibody is un-conjugated
(24), (22), (21), (14), (13), (13), (9), (8), (8), (8), (8), (8), (8), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(499), (286), (251), (148), (93), (88), (59), (50), (19), (14), (11), (9), (1), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-RPS6KB1 Antibody

Target Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 Antibody (ABIN969565) Images
Purification Purified
Immunogen Purified recombinant fragment of human RPS6KB1 expressed in E. coli.
Clone 5G9
Isotype IgG1

Target Details RPS6KB1

Product Details anti-RPS6KB1 Antibody Application Details Handling References for anti-RPS6KB1 Antibody (ABIN969565) Images back to top
Antigen
Alternative Name RPS6KB1 (RPS6KB1 Antibody Abstract)
Background This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates several residues of the S6 ribosomal protein. The kinase activity of this protein leads to an increase in protein synthesis and cell proliferation. Amplification of the region of DNA encoding this gene and overexpression of this kinase are seen in some breast cancer cell lines. Alternate translational start sites have been described and alternate transcriptional splice variants have been observed but have not been thoroughly characterized.
Molecular Weight 59 kDa
Gene ID 6198
HGNC 6198
Research Area Signaling, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Handling References for anti-RPS6KB1 Antibody (ABIN969565) Images back to top
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only

Handling

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details References for anti-RPS6KB1 Antibody (ABIN969565) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide and 0.5 % protein stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.

References for anti-RPS6KB1 Antibody (ABIN969565)

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling Images back to top
Product cited in:

Jan, Adolfsson, Allaman, Buccarello, Magistretti, Pfeifer, Muhs, Lashuel: "Abeta42 neurotoxicity is mediated by ongoing nucleated polymerization process rather than by discrete Abeta42 species." in: The Journal of biological chemistry, Vol. 286, Issue 10, pp. 8585-96, 2011

Deshmukh, Salehzadeh, Metayer-Coustard, Fahlman, Nair, Al-Khalili: "Post-transcriptional gene silencing of ribosomal protein S6 kinase 1 restores insulin action in leucine-treated skeletal muscle." in: Cellular and molecular life sciences : CMLS, Vol. 66, Issue 8, pp. 1457-66, 2009

Images

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 Antibody (ABIN969565) back to top
Supplier Images
ELISA image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (ABIN969565) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (ABIN969565) Figure 2: Flow cytometric analysis of Jurkat cells using RPS6KB1 mouse mAb (green) an...
Immunohistochemistry (IHC) image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (ABIN969565) Figure 3: Immunohistochemical analysis of paraffin-embedded breast cancer tissues usi...
Immunohistochemistry (IHC) image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (ABIN969565) Figure 4: Immunohistochemical analysis of paraffin-embedded muscle tissues using RPS6...
Western Blotting (WB) image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (ABIN969565) Figure 5: Western blot analysis using RPS6KB1 mAb against human RPS6KB1 (AA: 295-524)...