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anti-Rat (Rattus) RPS6KB1 antibody for Enzyme Immunoassay

Recommended RPS6KB1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
  • PS6K
  • S6K
  • S6K-beta-1
  • S6K1
  • STK14A
  • p70 S6KA
  • p70(S6K)-alpha
  • p70-S6K
  • p70-alpha
  • p70S6K
  • 2610318I15Rik
  • 4732464A07Rik
  • 70kDa
  • AA959758
  • AI256796
  • AI314060
  • p70/85s6k
  • p70s6k
  • ribosomal protein S6 kinase, 70kDa, polypeptide 1
  • ribosomal protein S6 kinase, polypeptide 1
  • RPS6KB1
  • Rps6kb1
Epitope
C-Term
99
32
27
26
24
24
20
20
19
18
18
18
18
16
15
15
15
14
14
10
10
8
7
6
6
5
5
5
5
5
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
656
436
414
72
53
40
34
33
21
13
13
8
5
5
3
3
1
Host
Goat
591
46
22
2
Clonality
Polyclonal
Conjugate
This RPS6KB1 antibody is un-conjugated
24
22
21
14
13
13
9
8
8
8
8
8
8
8
8
7
7
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
507
265
244
140
115
102
64
36
20
17
15
11
7
1
1
1
1
Supplier
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Catalog No. ABIN374267
$ 379.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN264871 EIA IP WB Rabbit IgG C-Term Log in to see Polyclonal 4
1 ABIN401355 EIA IP WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN303144 EIA IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal

Top referenced anti-RPS6KB1 antibody for Enzyme Immunoassay

Similar anti-RPS6KB1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Dog (Canine) Cow (Bovine)
Immunofluorescence (fixed cells) (IF/ICC) 17 Antibodies 15 Antibodies 15 Antibodies 6 Antibodies 9 Antibodies
Simple Western (SimWes) 1 Antibodies 1 Antibodies 1 Antibodies
Western Blotting (WB) 502 Antibodies 312 Antibodies 292 Antibodies 69 Antibodies 39 Antibodies
Radioimmunoassay (RIA) 1 Antibodies 1 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
Epitope C-Term
(99), (32), (27), (26), (24), (24), (20), (20), (19), (18), (18), (18), (18), (16), (15), (15), (15), (14), (14), (10), (10), (8), (7), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(656), (436), (414), (72), (53), (40), (34), (33), (21), (13), (13), (8), (5), (5), (3), (3), (1)
Host Goat
(591), (46), (22), (2)
Clonality
Polyclonal
Conjugate This RPS6KB1 antibody is un-conjugated
(24), (22), (21), (14), (13), (13), (9), (8), (8), (8), (8), (8), (8), (8), (8), (7), (7), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(507), (265), (244), (140), (115), (102), (64), (36), (20), (17), (15), (11), (7), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-RPS6KB1 Antibody

Target Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 Antibody (ABIN374267) Images
Sequence C-MISKRPEHLR MNL
Specificity This antibody detects RPS6KB1 at C-term.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
Immunogen Peptide from the C Terminus of the protein sequence according to NP_003152.1 Genename: RPS6KB1

Target Details RPS6KB1

Product Details anti-RPS6KB1 Antibody Application Details Handling References for anti-RPS6KB1 Antibody (ABIN374267) Images back to top
Antigen
Alternative Name RPS6KB1 / STK14A (RPS6KB1 Antibody Abstract)
Background The protein p70 S6 kinase is critical for cell cycle progression and cell survival. In response to mitogen stimulation, p70 S6 kinase activation up-regulates ribosomal biosynthesis and enhances the translational capacity. p70S6K phosphorylates the S6 protein of the 40S subunit of the ribosome. This kinase was first characterized as an insulin/mitogen-activated protein kinase, whose major known substrate is the 40 S ribosomal subunit protein S6. The p70 S6 kinase is activated by diverse stimuli through a multi-site phosphorylation such as Thr-252 and Ser-434. In Alzheimer's Disease (AD), p70 S6 kinase activation is associated with PHF-tau (hyperphosphorylated tau) accumulation. In non-neuronal cells, p70 S6 kinase have been shown to regulate actin polymerization.Synonyms: 70 kDa ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase I, Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, Serine/threonine-protein kinase 14A, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, p70-S6K 1
Molecular Weight 60kDa according to NP_003152.1
Gene ID 6198
NCBI Accession NP_003152
UniProt P23443
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Smooth Muscle Cell Migration

Application Details

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Handling References for anti-RPS6KB1 Antibody (ABIN374267) Images back to top
Application Notes Peptide ELISA: Detection Limit: 1/128000. Western blot: 0.2-1 μg/mL. Detects a band of Approx 65-70 kDa in Human Skeletal Muscle and Placenta lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details References for anti-RPS6KB1 Antibody (ABIN374267) Images back to top
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-RPS6KB1 Antibody (ABIN374267)

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling Images back to top
Background publications

Grove, Banerjee, Balasubramanyam, Coffer, Price, Avruch, Woodgett: "Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini." in: Molecular and cellular biology, Vol. 11, Issue 11, pp. 5541-50, 1991

Images

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 Antibody (ABIN374267) back to top
Supplier Images
 image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (C-Term) (ABIN374267) anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (C-Term) antibody
 image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (C-Term) (ABIN374267) anti-Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) (C-Term) antibody (Image 2)