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anti-Rat (Rattus) RPS6KB1 antibody for ELISA

Recommended RPS6KB1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
  • 70kDa
  • 2610318I15Rik
  • 4732464A07Rik
  • AA959758
  • AI256796
  • AI314060
  • p70 S6KA
  • p70(S6K)-alpha
  • p70-alpha
  • p70-S6K
  • p70/85s6k
  • p70S6K
  • p70s6k
  • PS6K
  • S6K
  • S6K-beta-1
  • S6K1
  • STK14A
Epitope
pThr389
109
35
31
29
24
24
21
19
18
18
18
17
16
15
14
13
13
13
13
11
8
8
8
7
6
5
5
5
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
649
359
344
48
30
21
16
15
6
1
1
1
1
Host
Rabbit
591
40
25
2
Clonality
Polyclonal
Conjugate
This RPS6KB1 antibody is un-conjugated
24
22
21
13
13
13
8
8
8
8
8
8
8
8
8
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
497
282
250
147
93
88
59
50
19
11
10
9
1
1
1
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Catalog No. ABIN1531360
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012155735 ABIN1533663 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.0012155735 ABIN1533098 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
0.0012155735 ABIN361700 ICC IF IP ELISA WB Rabbit C-Term Log in to see Polyclonal 6
0.0012155735 ABIN2486867 ICC IF IP ELISA WB Atto 390 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486868 ICC IF IP ELISA WB Atto 488 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486869 ICC IF IP ELISA WB Atto 565 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486870 ICC IF IP ELISA WB Atto 594 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486871 ICC IF IP ELISA WB Atto 633 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486872 ICC IF IP ELISA WB Atto 655 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486874 ICC IF IP ELISA WB Atto 700 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486873 ICC IF IP ELISA WB Atto 680 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486875 ICC IF IP ELISA WB Alkaline Phosphatase (AP) Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486876 ICC IF IP ELISA WB APC Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486877 ICC IF IP ELISA WB Biotin Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486878 ICC IF IP ELISA WB FITC Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486880 ICC IF IP ELISA WB PE-Atto 594 Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486879 ICC IF IP ELISA WB HRP Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486881 ICC IF IP ELISA WB PerCP Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486882 ICC IF IP ELISA WB PE Rabbit C-Term Log in to see Polyclonal 3
0.0012155735 ABIN2486883 ICC IF IP ELISA WB Streptavidin Rabbit C-Term Log in to see Polyclonal 3

Top referenced anti-RPS6KB1 antibody for ELISA

Similar anti-RPS6KB1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 95 Antibodies 98 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies 5 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 11 Antibodies 11 Antibodies
Immunocytochemistry (ICC) 34 Antibodies 35 Antibodies
Immunofluorescence (IF) 103 Antibodies 105 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 88 Antibodies 88 Antibodies
Immunohistochemistry (IHC) 153 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 49 Antibodies
Immunoprecipitation (IP) 35 Antibodies
Western Blotting (WB) 241 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
Epitope pThr389
(109), (35), (31), (29), (24), (24), (21), (19), (18), (18), (18), (17), (16), (15), (14), (13), (13), (13), (13), (11), (8), (8), (8), (7), (6), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(649), (359), (344), (48), (30), (21), (16), (15), (6), (1), (1), (1), (1)
Host Rabbit
(591), (40), (25), (2)
Clonality
Polyclonal
Conjugate This RPS6KB1 antibody is un-conjugated
(24), (22), (21), (13), (13), (13), (8), (8), (8), (8), (8), (8), (8), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(497), (282), (250), (147), (93), (88), (59), (50), (19), (11), (10), (9), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-RPS6KB1 Antibody

Target Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 Antibody (ABIN1531360) Images
Specificity P70 S6 Kinase (Phospho-Thr389) Antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at Thr389.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of Thr389.
Isotype IgG

Target Details RPS6KB1

Product Details anti-RPS6KB1 Antibody Application Details Handling References for anti-RPS6KB1 Antibody (ABIN1531360) Images back to top
Antigen
Alternative Name p70 S6 Kinase (RPS6KB1 Antibody Abstract)
Background Molecular weight of target protein: 59 kDa
Synonyms: Ribosomal protein S6 kinase I, S6K, S6K1, 70 kDa ribosomal protein S6 kinase 1, p70 S6 kinase alpha, p70(S6K)-alpha, p70-S6K, P70S6K, p70-alpha
Molecular Weight 59 kDa
Gene ID 6198
OMIM 608938
UniProt P23443
Research Area Signaling, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling

Application Details

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Handling References for anti-RPS6KB1 Antibody (ABIN1531360) Images back to top
Application Notes WB: 1:500-1:1000
IHC: 1:50-1:100
IF: 1:100-1:500
ELISA: 1:1000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.463642 (NCBI Gene Symbol: RPS6KB1)

Restrictions For Research Use only

Handling

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details References for anti-RPS6KB1 Antibody (ABIN1531360) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-RPS6KB1 Antibody (ABIN1531360)

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling Images back to top
Product cited in:

Chan, Wang, Fang, Chen: "Fibrous papule of the face, similar to tuberous sclerosis complex-associated angiofibroma, shows activation of the mammalian target of rapamycin pathway: evidence for a novel therapeutic strategy?" in: PLoS ONE, Vol. 9, Issue 2, pp. e89467, 2014

Background publications

Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006

Images

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Handling References for anti-RPS6KB1 Antibody (ABIN1531360) back to top
Supplier Images
Western Blotting (WB) image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (pThr389) (ABIN1531360) Western blot analysis of extracts from Jurkat cells treated with Insulin 0.01U/ml 15'...
Immunohistochemistry (IHC) image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (pThr389) (ABIN1531360) Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p70 ...
Immunofluorescence (IF) image for anti-RPS6KB1 antibody (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1) (pThr389) (ABIN1531360) Immunofluorescence analysis of HuvEc cells, using p70 S6 Kinase (Phospho-Thr389) Anti...