anti-Rat (Rattus) RPS6KB1 antibody for Functional Studies

Recommended RPS6KB1 Antibody (supplied by: Log in to see )

Antigen
Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
  • PS6K
  • S6K
  • S6K-beta-1
  • S6K1
  • STK14A
  • p70 S6KA
  • p70(S6K)-alpha
  • p70-S6K
  • p70-alpha
  • p70S6K
  • 2610318I15Rik
  • 4732464A07Rik
  • 70kDa
  • AA959758
  • AI256796
  • AI314060
  • p70/85s6k
  • p70s6k
  • ribosomal protein S6 kinase, 70kDa, polypeptide 1
  • ribosomal protein S6 kinase, polypeptide 1
  • RPS6KB1
  • Rps6kb1
Epitope
AA 26-43
101
32
27
26
24
23
20
20
20
19
18
18
17
16
15
15
15
14
14
10
10
8
7
6
6
6
5
5
5
5
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
665
445
422
73
53
41
34
33
21
13
13
8
5
5
3
3
1
Host
Rabbit
593
52
23
2
Clonality
Polyclonal
Conjugate
This RPS6KB1 antibody is un-conjugated
24
22
21
14
13
13
9
9
9
9
9
8
8
8
8
8
8
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB)
508
265
244
140
116
104
63
36
21
20
17
11
6
1
1
1
Supplier
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Catalog No. ABIN211258
$ 837.83
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Similar anti-RPS6KB1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 110 Antibodies 118 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 4 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 11 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 103 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 104 Antibodies
Immunohistochemistry (IHC) 156 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 82 Antibodies
Immunoprecipitation (IP) 44 Antibodies
Radioimmunoassay (RIA) 1 Antibodies
Simple Western (SimWes) 1 Antibodies
Western Blotting (WB) 292 Antibodies

General

Antigen Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1) Antibodies
Epitope AA 26-43
(101), (32), (27), (26), (24), (23), (20), (20), (20), (19), (18), (18), (17), (16), (15), (15), (15), (14), (14), (10), (10), (8), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(665), (445), (422), (73), (53), (41), (34), (33), (21), (13), (13), (8), (5), (5), (3), (3), (1)
Host Rabbit
(593), (52), (23), (2)
Clonality
Polyclonal
Conjugate This RPS6KB1 antibody is un-conjugated
(24), (22), (21), (14), (13), (13), (9), (9), (9), (9), (9), (8), (8), (8), (8), (8), (8), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Immunoprecipitation (IP), Western Blotting (WB)
(508), (265), (244), (140), (116), (104), (63), (36), (21), (20), (17), (11), (6), (1), (1), (1)
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Product Details anti-RPS6KB1 Antibody

Target Details RPS6KB1 Application Details Handling Images
Specificity Recognizes p70 S6 kinase Mr ~64kD. In some cell lysates, an additional protein may be detected at a higher molecular weight. Cross-reacts with rat, human and mouse.
Purification Protein A purified
Immunogen Synthetic peptide corresponding to residues 26-43 of rat S6 Kinase. The immunizing sequence is identical in mouse and human.

Type of Immunogen: Synthetic peptide
Isotype IgG

Target Details RPS6KB1

Product Details anti-RPS6KB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name RPS6KB1 / P70S6K / S6K (RPS6KB1 Antibody Abstract)
Background Name/Gene ID: RPS6KB1
Subfamily: P70 RSK
Family: Protein Kinase

Synonyms: RPS6KB1, 70 kDa S6 kinase, p70 S6KA, p70-alpha, p70-S6K, p70 S6 kinase alpha, p70s6 kinase, S6K-beta-1, STK14A, p70 ribosomal S6 kinase, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, p70 S6K-alpha, p70-S6K 1, p70S6K, p70S6K1, PS6K, Ribosomal protein S6 kinase I, S6K, S6K1, p70 ribosomal S6 kinase alpha, p70(S6K)-alpha
Gene ID 6198
UniProt P23443
Pathways PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Smooth Muscle Cell Migration

Application Details

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Handling Images back to top
Application Notes Approved: Func, IP, WB

Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot Analysis: 0.5-2.0 μg/mL detects p70 S6 kinase in RIPA lysates from HEK293, PC-12, and A431 cells. HEK293 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-p70 S6 kinase (2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 μg immunoprecipitates p70 S6 kinase from 500 μg of HEK293 RIPA lysate. Immunoprecipitation Kinase Assay: 5 μg immunoprecipitates active p70 S6 kinase from IGF-1 stimulated (15nM) human HEK293 cell lysate containing 1 uM microcystin. The assay was performed using a S6 Kinase Assay Kit, which uses specific substrate peptide as a kinase substrate and contains a separate inhibitor cocktail which blocks the activity of other serine/threonine kinases such as protein kinase A, protein kinase C, and calmodulin dependent kinases.
Restrictions For Research Use only

Handling

Product Details anti-RPS6KB1 Antibody Target Details RPS6KB1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeat freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment Long term: -20°C
Short term: +4°C. Avoid repeat freeze-thaw cycles.